Immature Embryo Rescue and in Vitro Development Evaluation of Intraspecific Hybrids from Brazilian Seedless Grapevine “Superior × Thompson” Clones


The fruit production for export is an economically significant activity in the Valley of S?o Francisco River, especially in the irrigated lands of Petrolina-PE/Juazeiro-BA, Brazil. The development of new genetic material most suitable to the tropical climate and the demands of the consumer market have led to the selection of new seedless grapes cultivars. In this case, the use of the embryo rescue technique has produced satisfactory results for obtaining such materials, especially in the semiarid region. This study aimed to evaluate the in vitro development of intraspecific hybrids of grapevine (Vitis vinifera L.), derived from the rescue of immature embryos resultant from the crossing of “Superior Seedless” and “Thompson Seedless” Brazilian clones. To establish and develop the cultivation, the culture media was supplemented with 30 g/L sucrose, 0.1 g/L myo-inositol, 0.002 g/L glycine, 0.1 mg/L indoleacetic acid (IAA), 6.5 g/L of agar, adjusted pH to 5.7. The experiment was evaluated after 90 days. The variables measured were: number of nodes, number of leaves, plant height (cm), number of roots and length (cm) of the root system and internodes. The period of 60 days of in vitro culture of ovules resulted in the highest values of embryos (about 50%), as well as better characterized developmental stages with higher germination (47.3%). The three types of hybrid grapes evaluated in micropropagation showed very similar values of the measured parameters, even having originated from embryos of different developmental stages.

Share and Cite:

Menezes, E. , Silva, E. , Yano-Melo, A. , Leão, P. and Melo, N. (2014) Immature Embryo Rescue and in Vitro Development Evaluation of Intraspecific Hybrids from Brazilian Seedless Grapevine “Superior × Thompson” Clones. American Journal of Plant Sciences, 5, 1956-1960. doi: 10.4236/ajps.2014.513209.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Baletti, B. (2013) Workers, State, and Development in Brazil: Powers of Labour, Chains of Value. The Journal of Peasant Studies, 40, 783-786.
[2] Araújo, E.P. and Araújo, J.L.P. (2006) Análise do custo de produção e rentabilidade do cultivo da uva fina de mesa produzida na região do Submédio São Francisco. Anais do 13 Simpósio de Engenharia de Produção: Empreendedorismo e Sustentabilidade nos Sistemas Produtivos, Bauru.
[3] Camargo, U.A., Maia, J.D.G., Quecini, V. and Ritschel, P. (2010) Brazilian Grape Breeding Program. In: Proceedings of the 10th International Conference on Grapevine Breeding and Genetics, Genova, 1-5 August 2010, 32-33.
[4] MELO, N.F. (2004) Contribuição da biotecnologia no desenvolvimento da viticultura no Vale do São Francisco. In: Anais do Seminário Novas Perspectivas para o Cultivo da Uva sem Sementes no Vale do São Francisco, 15-16 June 2004, Petrolina, 91-95.
[5] Li, R.G.R., Ji, W., Wang, G., Zhang, J.X. and Wang, Y.J. (2013) An Improved Embryo-Rescue Protocol for Hybrid Progeny from Seedless Vitis vinifera Grapes × Wild Chinese Vitis Species. In Vitro Cellular & Developmental Biology—Plant, 50, 110-120.
[6] Gonçalves, N.P.S., Borges, R.M.E., Gomes, A.P.O., Alves, E.O.S. and Leão, P.C.S. (2007) Evaluation of Grape Hybrids Obtained by Controlled Pollination. In: Anais da 2a Jornada de Iniciação Científica da Embrapa Semiárido, Petrolina, 135-140.
[7] Pommer, C.V., Ramming, D.W. and Emershad, R.L. (1995) Influence of Grape Genotype, Ripening Season, Seed Trace Size, and Culture Date on in Ovule Embryo Development and Plant Formation. Bragantia, 54, 237-249.
[8] Galzy, R. (1964) Technique de thermothérapiedesviruses de lavigne. Annalesdes Epiphyties, 15, 245-256.
[9] Ferreira, D.F. (2000) Sistemas de análise estatística para dados balanceados. UFLA/DEX/SISVAR.
[10] Valdez, J.G. (2005) Immature Embryo Rescue of Grapevine (Vitis vinifera L.) after an Extended Period of Seed Trace Culture. Vitis, 44, 17-23.
[11] Tian, L.L., Wang, Y.J., Niu, L. and Tang, D.M. (2008) Breeding of Disease-Resistant Seedless Grapes Using Chinese Wild Vitis spp. I. In Vitro Embryo Rescue and Plant Development. Scientia Horticulturae, 117, 136-141.
[12] Yang, D.L., Li, W., Li, S., Yang, X.L., Wu, J.L. and Cao, Z.Y. (2007) In Vitro Embryo Rescue Culture of F1 Progenies from Crosses between Diploid and Tetraploid Grape Varieties. Plant Growth Regulation, 51, 63-71.
[13] Bharathy, P.V., Karibasappa, G.S., Patil, S.G. and Agrawal, D.C. (2005) In Ovulo Rescue of Hybrid Embryos in Flame Seedless Grapes—Influence of Pre-Bloom Sprays of Benzyladenine. Scientia Horticulturae, 106, 353-359.
[14] Ramming, D.W., Emershad, R.L. and Tarailo, R. (2000) A Stenospermocarpic, Seedless Vitis viniferax Vitisrotundifolia Hybrid Developed by Embryo Rescue. HortScience, 35, 732-734.
[15] Singh, N.V., Singh, S.K. and Singh, A.K. (2011) Standardization of Embryo Rescue Technique and Bio-Hardening of Grape Hybrids (Vitis vinifera L.) using Arbuscular Mycorrhizal Fungi (AMF) under Sub-Tropical Conditions. Vitis, 50, 115-118.
[16] Tang, D.M., Wang, Y.J., Cai, J.S. and Zhao, R.H. (2009) Effects of Exogenous Application of Plant Growth Regulators on the Development of Ovule and Subsequent Embryo Rescue of Stenospermic Grape (Vitis vinifera L.). Scientia Horticulturae, 120, 51-57.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.