Micro-Computed Tomography Provides Accurate Measurement for Cardiac Function in Infarcted Rat Heart


Objective: High resolution image is required for accurate measurement of cardiac function for the analysis of detailed regional function especially in a small animal. Methods: Left ventricular function of rat hearts was measured using micro-computed tomography (micro-CT) with administration of contrast agent in a rat with normal heart as well as rats with mild and severe myocardial infarction (MI). Following the CT acquisition, the hearts were sectioned for pathological evaluation. Results: The volume plot per each disk of the normal heart revealed that contraction force peaked at the middle of the heart. In the heart with mild infarction, the volume plot curve clearly demonstrated that infarction was located only at the apex of the heart, whereas severe infarction was disturbed in larger area. The left ventricular ejection fraction of the normal, mild MI, and severe MI hearts were 68.6%, 40.0%, and 16.4%, respectively. In addition, volume analysis in severe MI demonstrated ventricular dilatation, although that in mild MI did not show any change in the ventricular volume. Histological results were consistent with the CT measurement. Conclusions: Micro-CT provided accurate measurement of cardiac function in rats, which is especially useful for the analysis of small animals with heterogeneous dysfunction of the heart.

Share and Cite:

Matsushita, S. , Naito, M. and Amano, A. (2014) Micro-Computed Tomography Provides Accurate Measurement for Cardiac Function in Infarcted Rat Heart. Open Journal of Medical Imaging, 4, 72-79. doi: 10.4236/ojmi.2014.42010.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Dewey, M., Müller, M., Eddicks, S., et al. (2006) Evaluation of Global and Regional Left Ventricular Function with 16-Slice Computed Tomography, Biplane Cineventriculography, and Two-Dimensional Transthoracic Echocardiography: Comparison with Magnetic Resonance Imaging. Journal of the American College of Cardiology, 48, 2034-2044.
[2] Bellenger, N.G., Burgessm, M.I., Ray, S.G., et al. (2000) Comparison of Left Ventricular Ejection Fraction and Volumes in Heart Failure by Echocardiography, Radionuclide Ventriculography and Cardiovascular Magnetic Resonance; Are They Interchangeable? European Heart Journal, 21, 1387-1396.
[3] Buck, T., Hunold, P., Wentz, K.U., et al. (1997) Tomographic Three-Dimensional Echocardiographic Determination of Chamber Size and Systolic Function in Patients with Left Ventricular Aneurysm: Comparison to Magnetic Resonance Imaging, Cineventriculography, and Two-Dimensional Echocardiography. Circulation, 96, 4286-4297.
[4] Greupner, J., Zimmermann, E., Grohmann, A., et al. (2012) Head-to-Head Comparison of Left Ventricular Function Assessment with 64-Row Computed Tomography, Biplane Left Cineventriculography, and Both 2- and 3-Dimensional Transthoracic Echocardiography: Comparison with Magnetic Resonance Imaging as the Reference Standard. Journal of the American College of Cardiology, 59, 1897-1907.
[5] Zimmer, H.G. and Millar, H.D. (1998) Technology and Application of Ultraminiature Catheter Pressure Transducers. The Canadian Journal of Cardiology, 14, 1259-1266.
[6] Nielsen, J.M., Kristiansen, S.B., Ringgaard, S., et al. (2000) Left Ventricular Volume Measurement in Mice by Conductance Catheter: Evaluation and Optimization of Calibration. The American Journal of Physiology—Heart and Circulatory Physiology, 293, H534-H540.
[7] Ishizu, K., Mukai, T., Yonekura, Y., et al. (1995) Ultra-High Resolution SPECT System Using Four Pinhole Collimators for Small Animal Studies. Journal of Nuclear Medicine, 36, 282-287.
[8] Visser, E.P., Disselhorst, J.A., Brom, M., et al. (2009) Spatial Resolution and Sensitivity of the Inveon Small-Animal PET Scanner. The Journal of Nuclear Medicine, 50, 139-147.
[9] Gullberg, G.T., Reutter, B.W., Sitek, A., et al. (2010) Dynamic Single Photon Emission Computed Tomography—Basic Principles and Cardiac Applications. Physics in Medicine and Biology, 55, R111-R191.
[10] Golestani, R., Wu, C., Tio, R.A., et al. (2010) Small-Animal SPECT and SPECT/CT: Application in Cardiovascular Research. European Journal of Nuclear Medicine and Molecular Imaging, 37, 1766-1777.
[11] Hayasaka, N., Nagai, N., Kawao, N., et al. (2012) In Vivo Diagnostic Imaging Using Micro-CT: Sequential and Comparative Evaluation of Rodent Models for Hepatic/Brain Ischemia and Stroke. PLoS One, 7, e32342.
[12] Detombe, S.A., Ford, N.L., Xiang, F., et al. (2008) Longitudinal Follow-Up of Cardiac Structure and Functional Changes in an Infarct Mouse Model Using Retrospectively Gated Micro-Computed Tomography. Investigative Radiology, 43, 520-529.
[13] Nahrendorf, M., Badea, C., Hedlund, L.W., et al. (2007) High-Resolution Imaging of Murine Myocardial Infarction with Delayed-Enhancement Cine Micro-CT. The American Journal of Physiology—Heart and Circulatory Physiology, 292, H3172-H3178.
[14] Drangova, M., Ford, N.L., Detombe, S.A., et al. (2007) Fast Retrospectively Gated Quantitative Four-Dimensional 4D Cardiac Micro Computed Tomography Imaging of Free-Breathing Mice. Investigative Radiology, 42, 85-94.
[15] Badea, C.T., Fubara, B., Hedlund, L.W., et al. (2005) 4-D Micro-CT of the Mouse Heart. Molecular Imaging, 4, 110- 116.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.