Meizothrombin Preparation and Its Role in Fibrin Formation and Platelet Aggregation

DOI: 10.4236/abb.2014.57069   PDF   HTML   XML   2,987 Downloads   4,104 Views   Citations


Meizothrombin (MT) is one of prothrombin derivatives which appears in haemostasis activation area. However, its role in haemostasis regulation isn’t clear. We studied the role of MT in fibrin formation, platelet activation and aggregation. A new effective method of obtaining MT from native human prothrombin was developed using immobilised prothrombin activator from Echis multisquamatis venom. The protein was stable and electrophoretically pure. Platelet-rich plasma for aggregation study and gel-sieved platelets for flow-cytometry were separated from blood of healthy donors. It was shown that MT transformed fibrinogen to fibrin and activated clotting factor XIII. MT didn’t activate gel-sieved intact platelets, but in platelet-rich plasma, increased platelet aggregation induced by ADP, collagen and adrenalin.

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Korolova, D. , Chernyshenko, T. , Gornytska, O. , Chernyshenko, V. and Platonova, T. (2014) Meizothrombin Preparation and Its Role in Fibrin Formation and Platelet Aggregation. Advances in Bioscience and Biotechnology, 5, 588-595. doi: 10.4236/abb.2014.57069.

Conflicts of Interest

The authors declare no conflicts of interest.


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