Calibration of CO2 Trapping in Alkaline Solutions during Soil Incubation at Varying Temperatures Using a Respicond VI

DOI: 10.4236/ojss.2014.45019   PDF   HTML   XML   4,768 Downloads   6,541 Views   Citations


Measurements of carbon dioxide (CO2)-evolution from soils are important in evaluating biomass and activity of soil microorganisms, as well as decomposition of soil organic matter. The Respicond VI is a fully computerized system allowing continuous measurement of CO2 evolution in short- and long-term soil incubation experiments in up to 96 incubation vessels. The measurement of CO2 evolution is based on the absorption of CO2 by an electrolyte (KOH solution) producing a change in the cell conductance measured using two electrodes. In this study, the Respicond VI was recalibrated yielding 174.5 mg CO2 as constant A expressing the theoretical maximum amount of CO2 absorbed in 10 ml 0.5 M KOH. This value of A corresponds to 34.9 mg CO2 ml-1 1 M KOH. The constant A does neither depend on the investigated incubation temperatures (5°C - 25°C) nor on the concentrations of the KOH solutions (0.5, 0.1, 0.05 M KOH). To eliminate any influence of changing incubation temperatures, either induced by uncertainties in temperature control or as a part of the experimental setup, on the conductance of KOH solution, a correction procedure was developed using a factor calculated from changing conductance of KOH solutions in incubation vessels without soil.

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Smirnova, N. , Demyan, M. , Rasche, F. , Cadisch, G. and Müller, T. (2014) Calibration of CO2 Trapping in Alkaline Solutions during Soil Incubation at Varying Temperatures Using a Respicond VI. Open Journal of Soil Science, 4, 161-167. doi: 10.4236/ojss.2014.45019.

Conflicts of Interest

The authors declare no conflicts of interest.


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