Evaluation of Whole Saliva Antioxidant Capacity in Patients with Periodontal Diseases


Background and purpose: Periodontitis is the inflammation of the supporting tissues of teeth. In this disease, overproduction of free radicals by polymorphonuclears (PMNs) will result in the imbalance between saliva’s oxidants and antioxidants. Therefore, due to insufficient levels of anti-oxidants, free radicals can start chain reactions that eventually will lead to destruction of periodontal tissues. The purpose of this research is to compare the amount of whole saliva’s antioxidant capacity in people with major periodontitis with that of healthy people. Methods and materials: In this case-control study, the amount of antioxidants capacity and saliva antioxidants were measured in 43 cases with periodontitis, as well as in 43 healthy people (controls); and the measurements were compared between the two groups. The amount of antioxidants capacity and their saliva antioxidants capacity was measured by FRAP (ferric reducing ability of plasma) and with the help of a Spectrophotometer. Findings: Average and Standard deviation of saliva’s antioxidants was 184.25 ± 63.209 in subject group and 399.419 ± 90.298 micro moles in control group. Using the T-TEST revealed that the difference in capacity of whole saliva’s antioxidants in two groups is significant. Conclusion: whole saliva’s antioxidants capacity is less among patients with periodontitis compared to control group.

Share and Cite:

Azizi, A. , Sarlati, F. , Parchakani, A. and Alirezaei, S. (2014) Evaluation of Whole Saliva Antioxidant Capacity in Patients with Periodontal Diseases. Open Journal of Stomatology, 4, 228-231. doi: 10.4236/ojst.2014.44031.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Loesche, W.J. and Grossman, N.S. (2001) Periodontal Disease as a Specific, Albeit Chronic, Infection: Diagnosis and Treatment. Clinical Microbiology Reviews, 14, 727-752.
[2] Kim, S.C., Kim, O.S., Kim, O.J., Kim, Y.J. and Chung, H.J. (2010) Antioxidant Profile of Whole Saliva after Scaling and Root Planning in Periodontal Disease. Journal of Periodontal & Implant Science, 40, 164-171.
[3] Diab-Ladki, R., Pellat, B. and Chahine, R. (2003) Decrease in the Total Antioxidant Activity of Saliva in Patients with Periodontal Diseases. Clinical Oral Investigations, 7, 103-107.
[4] Wei, D., Zhang, X.L., Wang, Y.Z., Yang, C.X. and Chen, G. (2010) Lipid Peroxidation Levels, Total Oxidant Status and Superoxide Dismutase in Serum, Saliva and Gingival Crevicular Fluid in Chronic Periodontitis Patients before and after Periodontal Therapy. Australian Dental Journal, 55, 70-78.
[5] Nikolaev, I.V., Kolobkova, L.N., Landesman, E.O., Stepanov, E.V. and Koroleva, O.V. (2008) Antioxidant and Peroxidase Activity of Saliva in Patients with Inflammatory Periodontal Diseases and Ability of Their Correction. Biomed Khim, 54, 454-462.
[6] Azizi, A. and Farshchi, F. (2012) Comparison of Salivary and Plasma Antioxidant Levels in Lichen Planus Patients and Healthy Subjects. Journal of Oral Pathology & Medicine, 41, 524-526.
[7] Akalin, F.A., Baltacioglu, E., Alver, A. and Karabulut, E. (2007) Lipid Peroxidation Levels and Total Oxidant Status in Serum, Saliva and Gingival Crevicular Fluid in Patients with Chronic Periodontitis. Journal of Clinical Periodontology, 34, 558-565.
[8] Baltacioglu, E., Akalin, F.A., Alver, A., Balaban, F., Unsal, M. and Karabulut, E. (2006) Total Antioxidant Capacity and Superoxide Dismutase Activity Levels in Serum and Gingival Crevicular Fluid in Post-Menopausal Women with Chronic Periodontitis. Journal of Clinical Periodontology, 33, 385-392.
[9] Panjamurthy, K., Manoharan, S. and Ramachandran, C.R. (2005) Lipid Peroxidation and Antioxidant Status in Patients with Periodontitis. Cellular & Molecular Biology Letters, 10, 255-264.
[10] Sculley, D.V. and Langley-Evans, S.C. (2003) Periodontal Disease Is Associated with Lower Antioxidant Capacity in Whole Saliva and Evidence of Increased Protein Oxidation. Clinical Science, 105, 167-172.
[11] Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M. and Telser, J. (2007) Free Radicals and Antioxidants in Normal Physiological Functions and Human Disease. The International Journal of Biochemistry & Cell Biology, 39, 44-84.
[12] Benzie, I.F. and Strain, J.J. (1996) The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry, 239, 70-76.
[13] Moore, S., Calder, K.A., Miller, N.J. and Rice-Evans, C.A. (1994) Antioxidant Activity of Saliva and Periodontal Disease. Free Radical Research, 21, 417-425.
[14] Chapple, I.L., Mason, G.I., Garner, I., Matthews, J.B., Thorpe, G.H., Maxwell, S.R. and Whitehead, T.P. (1997) Enhanced Chemiluminescent Assay for Measuring the Total Antioxidant Capacity of Serum, Saliva and Crevicular Fluid. Annals of Clinical Biochemistry, 34, 412-421.
[15] Miricescu, D., Greabu, M., Totan, A., Didilescu, A. and Radulescu, R. (2011) The Antioxidant Potential of Saliva: Clinical Significance in Oral Diseases. Therapeutics, Pharmacology and Clinical Toxicology, 15, 139-143.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.