The antioxidative effects of some medicinal plants as hypoglycemic agents on chromosomal aberration and abnormal nucleic acids metabolism produced by diabetes stress in male adult albino rats

Sherifa H. Salah, Halima S. Abdou, Amal S. Abd El Azeem, E.A. Abdel-Rahim

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DOI: 10.4236/jdm.2011.11002                

Abstract

The objectives of the present study are to evaluate the chromosomal aberrations in somatic and germ cells of alloxanized diabetic rats treated with methanolic extracts of Cleome droserifolia (Sammo), Anastatica hierochuntica (Kafta), Juniperus phoencea (Araar) and Hyphaene thebaica (Doum) as well as their effects on blood glucose, liver and testes total soluble protein, DNA and RNA contents and also nucleases (RNAase and DNAase) activity. The material and Methods: The study was performed in six groups. The first group was the normal healthy control; the second group was rats injected with alloxan (diabetic control). Every one of the other four groups (diabetic rats) was ingested with each of the methanolic extracts of Kafta, Doum, Sammo and Araar (individually). The results: The results of chromosomal analysis showed that, diabetic rats had a highly significant increase of chromosomal aberrations compared to that of normal healthy control. Animals which treated with the four plants methanolic extracts showed significant improvements in the frequencies of chromosomal aberrations. In case of biochemical analysis, blood glucose level was significantly increased but immunoglobulins levels were decreased in diabetic albino rats. Diabetes increased serum total lipids, cholesterol, triglycerides and LDL but serum HDL was decreased. RNA and DNA as well as inhibited the nucleases (RNAase and DNAase) activity of both organs (liver and testes) tissue. The conclusions: It is concluded that diabetes is much harmful in the animal body, whilst the induction of flavonoids extract (by methanol) of Kafta, Somma, Araar and Doum reduced these harmful of diabetes.

Keywords

Alloxan: Recrystallized, Methanolic Extracts of Anastatica Hierochuntica (Kafta) Hyphaene Thebaica (Doum) Cleome Droserfolia (Sammo) Juniperus Phoenicea (Araar) and Rattus Norvegicus

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Caughron, K.F. and Smith, E.L. (2002) Definition and description of diabetes mellitus. South Medical Journal, 95, 35-49.
[2]	Kuzuya, T., Nakagaw, S., Satoh, J., Kanazawa, Y., Iwamoto, Y. and Kobayashi, M., et al. (2002) Report of the committee of the Japan Diabetes Society on the diagnostic criteria of diabetes mellitus. Diabetes Research and Clinical Practice, 55, 65-85. doi:10.1016/S0168-8227(01)00365-5
[3]	Lebvitz, H.E. (2001) Diagnosis, classification and pathogenesis of diabetes mellitus. The Journal of Clinical Psychiatry, 62, 5-9.
[4]	Lotito, S.B. and Frei, B. (2004) Relevance of apple polyphenols as antioxidants in human plasma: Contrasting in vitro and in vivo effects. Free Radical Biology and Medicine, 36, 201-211.
[5]	Ismail, S.H., Ahmed, S.A., Fayed, S.A. and Van-Breem- en, R.B. (2007) Adiponectin, insulin and nitric oxide levels in hyperlipidemia and hypercholesterolemia in addition of streprozotocin-induced diabetes mellitus and normal rats intraperitonially injected with anthocyanin and epicatechin extracts. New Egyptian Journal of Medicine, 36, 135-143.
[6]	Van-Lreshout, M., West, C.E. and Van-Breemen, R.B. (2003) Isotope tracer techniques for study the bioavailiability and inefficacy of dietary coroteniods, particulary B. The American. Journal of Clinical Nutrition, 7, 12-28.
[7]	Lane-Petter, W. and Pearson, A. (1971) Dietary requirement. In: The laboratory animal principles and practice. Academic press, London and New York, 142.
[8]	Lazaro, A. (1949) Alloxan and pathogenesis of diabetes mellitus. Physiological Reviews, 29, 48.
[9]	Abdel-Rahim, E.A., Salah, H., Sherifa, H.S., El-Beltagi S. and Fayed, A.S. (2005) Comparative studies on the influences of Juniperus phoenicea and Hyphaena thebaica as hypoglycemic factors in diabetic rats. Journal of Genetic Engineering and Biotechnology, 3, 2.
[10]	Yosida, T.H. and Amano, K. (1965) Autosomal polymorphism in laboratory bred and wild Norway rats Rattus norvegicus, found in Misima. Chromosoma, 16, 628-667. doi:10.1007/BF00285115
[11]	Brewen, G.J. and Preston, R.J. (1978) Analysis of chromosome aberration in mammalian germ cell. Chemical Mutagens, 5, 127-150.
[12]	Evans, E.P., Breckon, G.A. and Ford, C.E. (1964) An air-drying method for meiotic preparation from mammalian test. Cytogenetics, 3, 289-298. doi:10.1159/000129818
[13]	Trinder P. (1969) Enzymatic determination of glucose in blood serum. Annals of. Clinical Biochemistry, 6, 24.
[14]	Dische, Z., Chargaff, E. and Davidoson, J.M. (1955) Color reaction of nucleic acid components. In: The nucleic acids eds. Academic Press, New York, 1, 270-284.
[15]	Schneider, W. C. (1957) Determination of acid in tissues by pentose analysis. In: Method enzymology. Academic Press, New York, 680-384.
[16]	Lowary, O.H., Resabrought, N.J., Farrand, A.L. and Randoll, R.J. (1951) Protein measurements with the folin phenol reagent. The Journal of Biological Chemistry, 193, 267.
[17]	Bergmeyer, U.H. (1974) Determination of mucleases activity (RNAse and DNAase) In: Method of Enzymatic analysis. 2nd Edition, Acad. Press Inc., New York, San Francisco and London, 447-511.
[18]	Statistical package for the social science. (1999) Inc Chicago.
[19]	Snedecor, G.W. and Cochran, W.G. (1967) Statistical methods. 6th Edition, Ames, Lowa, the Lowa state University.
[20]	Ziyyat, A., Legssyer, A., Mekhfi, H., Dassouli, A., Serhr- ouchni, M. and Benjelloun, W. (1997) Phytotherpay of hypertension and diabetes in oriental Morocco. Journal of Ethnopharmacology, 58, 45-54. doi:10.1016/S0378-8741(97)00077-9
[21]	Hansen, M. (1998) Pathophysiology: Foundations of diseases and clinical intervention. W.B. Saunders Company, Philadelphia, U.S.A., 851-852.
[22]	Elsner, M., Guldbakke, B., Tiedge, M., Munday, R. and Lenzen, S. (2000) Relative importance of transport and alkylation for pancreatic beta-cell toxicity of streptozotocin. Diabetologia, 43, 1528-1533. doi:10.1007/s001250051564
[23]	Sennoune, S., Gerbi, A., Duran, M.J., Benkoel, L., Pierre, S., Lambert, R., et al. (1999) A quantitative immunocytochemical study of Na+, K+ ATPase in rat hepatocytes after STZ-induced diabetes and determination of blood glucose using an oxidase-peroxidase system: A non carcinogenic chromagen. Am. Clinical Biochemistry, 6, 24-30.
[24]	Rahmy, T.R. and El-Ridi, M.R. (2002) Action of Anastatica hierochuntica plant extract on Islets of Langerhans in normal and diabetic rats. Egyptian Journal of Biology, 4, 87-94.
[25]	Puri, D. and Baral, N. (1998) Hypoglycemic effect of Biophytum sensitivum in the alloxan diabetic rabbits. Indian Journal of Physiology & Pharmacology, 42, 401-406.
[26]	Das, A.V., Padayatti, P.S. and Paulose, C.S. (1996) Effect of leaf extract of Aegle marmelose (L.) Correa ex Roxb. on histological and ultrastructural changes in tissues of streptozotocin induced diabetic rats. Indian Journal of Experimental Biology, 34, 341-345.
[27]	Puri, D. and Baral, N. (1998) Hypoglycemic effect of Biophytum sensitivum in the alloxan diabetic rabbits. Indian Journal of Physiology & Pharmacology, 42, 401-406.
[28]	Khalifa, T.A. (1980) Pharmacognostical study of certain species of Anastatica. Ph.D. Thesis, Cairo University, Egypt.
[29]	Halima, S., Abdou, H., Sherifa, S. and Amira, A.R. (2008) Chromosomal aberrations and nucleic acids systems affected by some Egyptian medicinal plants used in treating female pregnant diabetic rats. Journal of Applied Science.
[30]	Wirasathian, L., Pengsuparp, T., Suttisri, R., et al., (2006) Inhibitors of aldose reductase and advanced glycation end-products formation from the leaves of Stelechocarpus Cauliflorus. Phytomedicine, Applied Science, 3.
[31]	Peyroux, J. and Sternberg, M. (2006) Advanced glycation end-products (AGEs): Pharmacological inhibition in diabetes. Pathological Biology, 54, 405-419. doi:10.1016/j.patbio.2006.07.006
[32]	Wirasathian, L., Pengsuparp, T., Suttisri, R., Ueda, H., Moriyasu, M. and Kawanishi, K. (2006) Inhibitors of aldose reductase and advanced glycation end-products formation from the leaves of Stelechocarpus Cauliflorus. Phytomedicine, Avilable online.
[33]	Coskun, O., Kanter, M., Korkmaz, A. and Oter, S. (2005) Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and β-cell damage in rat pancreas. Pharmacological research, 51, 117-123. doi:10.1016/j.phrs.2004.06.002
[34]	Anton, S., Melville, L. and Rena, G. (2007) Epigallocatechin gallate (EGCG) mimics insulin action on the transcription factor FOXOLa and elicits cellular responses in the presence and absence of insulin. Cellular signaling, 19, 378-383. doi:10.1016/j.cellsig.2006.07.008
[35]	Rao, T.P., Sakaguchi, N., Juneja, L.R., et al. (2005) (Emblica Officinalis Gaertn) extracts reduce oxidative stress in streptozotocin-induced diabetic rats. Journal of Medicinal Food, 8, 362-368. doi:10.1089/jmf.2005.8.362
[36]	Baraldi, R., Rapparini, F., Facini, O., et al. (2005) Isoprenoid emission and physiological activities of Mediterranean macchia vegetation under field condition. Mediterranean Ecology, 6, 3-9.
[37]	Spines, G.A. (1999) The dual role of nitric acid in islets β-cells. News in Physiological Sciences, 14, 49-54.
[38]	Adams, R.L.P., Knowler, J.T. and Leader. D.P. (1993) The biochemistry of nucleic acids. 11th Edition, Chepman and Hill publishers. Ltd. London, New York and Tokyo.
[39]	Nahla, S.S. and Abdel-Nabi, I.M. (2006) Hypoglycemic effect of Cleome droserifolia ethanolic leaf extract in experimental diabetes and on non-enzymatic antioxidant, glycogen, thyroid hormone and insulin levels. Diabetologia Croatica, 35, 15-22.
[40]	Al-Gaby, A.M.A. (1998) Amino acid compostion and biological effects of supplementing broad bean and corn protein. Nahrung, 42, 290-294. doi:10.1002/(SICI)1521-3803(199810)42:05<290::AID-FOOD290>3.0.CO;2-Y