Docosahexaenoic Acid Ester Degradation Measured by FTIR-ATR with Correlation Spectroscopy

DOI: 10.4236/ajac.2014.56045   PDF   HTML   XML   4,030 Downloads   4,989 Views  


Highly unsaturated fatty acids such as docosahexaenoic acid and linolenic acid are prone to oxidation with a resulting loss of bioactivity and generation of malodorous degradation compounds. Degradation proceeds by formation of the corresponding hydroperoxyl free radical with subsequent oxidative cleavage at the double bond site. FTIR-ATR was used to detect degradation through spectral changes that were correlated to oxidative degradation. The spectrometer was equipped with a heated stage so that a series of spectra could be collected while the sample was exposed to elevated temperatures. The appearance of new bands at 972.8 cm﹣1, 2934 cm﹣1, and 3498 cm﹣1 with the disappearance of bands at 703.6 cm﹣1 and 3013 cm﹣1 were observed after heating and these spectral changes were evaluated by correlation spectroscopy analysis. These results provide a method to quickly detect the oxidative degradation of polyunsaturated fats. This approach is expected to be applied for the quality assessment of feed formulations fortified with omega-3 fatty acids.

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Holser, R. (2014) Docosahexaenoic Acid Ester Degradation Measured by FTIR-ATR with Correlation Spectroscopy. American Journal of Analytical Chemistry, 5, 373-377. doi: 10.4236/ajac.2014.56045.

Conflicts of Interest

The authors declare no conflicts of interest.


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