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Involvement of GnRH and Gonadotropin Genes in Oocyte Development of Blue Gourami Females (Trichogaster trichopterus)

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DOI: 10.4236/abc.2014.43024    2,803 Downloads   4,148 Views   Citations
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The goal of this study was to gain a fundamental understanding of RnRH 1 and 3, as well as FSH and LH action, in ovarian follicles during oocyte growth by examining changes in ovarian gene transcription in blue gourami females (Trichogaster trichopterus). The levels of target ovarian mRNAs were determined by quantitative real-time PCR. GnRH1 mRNA levels were higher in oocytes at the previtellogenic stage compared to the vitellogenic and follicular oocytes maturation (FOM) stages. No significant differences in GnRH3 mRNA levels were detected among oocytes during the different stages of development. βLH and βFSH mRNA levels were lower in oocytes at the vitellogenic stage compared to the previtellogenic stage. The high transcription of GnRH 1 and 3 in the brain and of FSH and LH in the pituitary of blue gourami as was found in previous studies in our laboratory, compared to mRNA levels changed in ovary during oogenesis, supporting the hypothesis that differences exist between the function of endocrine and autocrine/paracrine of these hormones.

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Degani, G. (2014) Involvement of GnRH and Gonadotropin Genes in Oocyte Development of Blue Gourami Females (Trichogaster trichopterus). Advances in Biological Chemistry, 4, 197-202. doi: 10.4236/abc.2014.43024.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Yaron, Z., Gur, G., Melamed, P., Rosenfeld, H., Elizur, A. and Levavi-Sivan, B. (2003) Regulation of Fish Gonadotropins. International Review of Cytology, 225, 131-185.
[2] Kah, O., Lethimonier, C., Somoza, G., Guilgur, L.G., Vaillant, C. and Lareyre, J.J. (2007) GnRH and GnRH Receptors in Metazoa: A Historical, Comparative, and Evolutive Perspective. General and Comparative Endocrinology, 153, 346-364.
[3] Levy, G., Gothilf, Y. and Degani, G. (2009) Brain Gonadotropin Releasing Hormone3 Expression Variation during Oogenesis and Sexual Behavior and Its Effect on Pituitary Hormonal Expression in the Blue Gourami. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 154, 241-248.
[4] Parhar, I.S. (2002) Cell Migration and Evolutionary Significance of GnRH Subtypes. Progress in Brain Research, 141, 3-17.
[5] Levy, G. and Degani, G. (2012) Involvement of GnRH, PACAP and PRP in the Reproduction of Blue Gourami Females (Trichogaster trichopterus). Journal of Molecular Neuroscience: MN, 48, 603-616.
[6] Zohar, Y., Munoz-Cueto, J.A., Elizur, A. and Kah, O. (2010) Neuroendocrinology of Reproduction in Teleost Fish. General and Comparative Endocrinology, 165, 438-455.
[7] Jackson, K., Goldberg, D., Ofir, M., Abraham, M. and Degani, G. (1999) Blue Gourami (Trichogaster trichopterus) Gonadotropic Beta Subunits (I and II) cDNA Sequences and Expression during Oogenesis. Journal of Molecular Endocrinology, 23, 177-187.
[8] Wong, T.T. and Zohar, Y. (2004) Novel Expression of Gonadotropin Subunit Genes in Oocytes of the Gilthead Seabream (Sparus aurata). Endocrinology, 145, 5210-5220.
[9] Soverchia, L., Carotti, M., Andreu-Vieyra, C., Mosconi, G., Cannella, N., Habibi, H., et al. (2007) Role of Gonadotropin-Releasing Hormone (GnRH) in the Regulation of Gonadal Differentiation in the Gilthead Seabream (Sparus aurata). Molecular Reproduction and Development, 74, 57-67.
[10] Degani, G. (2001) Blue Gourami (Trichogaster trichopterus) Model for Labyrinth Fish. Laser Pages Publishing, Jerusalem.
[11] Degani, G. (1993) The Effect of Sexual Behaviour on Oocyte Development and Steroid Changes in Trichogaster trichopterus. Copeia, 4, 1091-1096.
[12] Degani, G. (1993) Reproduction Control in Multi-Spawning Asynchronic Trichogaster trichopterus (Pallas) as a Model for the Anabantidae Family. Threads in Comparative, Biochemistry and Physiology, 1, 1269-1275.
[13] Jackson, K., Degani, G., Bocker, R. and Abraham, M. (1998) Gonadotropin, Growth Hormone Cells and Oocyte Development in Female Gourami after Injection of Gonadotropin-Releasing Hormone Analogue. Journal of Aquaculture in the Tropics, 13, 159-170.
[14] Jackson, K., Goldberg, D., Ofir, M., Abraham, M. and Degani, G. (1999) Blue Gourami (Trichogaster trichopterus) Gonadotropic Beta Subunits (I and II) cDNA Sequences and Expression during Oogenesis. Journal of Molecular Endocrinology, 23, 177-187.
[15] Degani, G. and Boker, R. (1992) Sensitivity to Maturation Inducing Steroids and Gonadotropin in the Oocytes of Blue Gourami Trichogaster trichopterus, (Anabantidae, Pallas, 1770). General and Comparative Endocrinology, 85, 430-439.
[16] Goldberg, D., Jackson, K., Yom-Din, S. and Degani, G. (2004) Growth Hormone of Trichogaster trichopterus: cDNA Cloning, Sequencing and Analysis of mRNA Expression during Oogenesis. Journal of Aquaculture in the Tropics, 19, 215-229.
[17] Degani, G., Jackson, K., Yom-Din, S. and Goldberg, D. (2006) cDNA Cloning and mRNA Expression of Growth Hormone in Belontiidae (Anabantoidei suborder). The Israeli Journal of Aquaculture, 58, 124-136.
[18] Pfaffl, M.W. (2001) A New Mathematical Model for Relative Quantification in Real-Time RT-PCR. Nucleic Acids Research, 29, e45.
[19] Muller, P.Y., Janovjak, H., Miserez, A.R. and Dobbie, Z. (2002) Processing of Gene Expression Data Generated by Quantitative Real-Time RT-PCR. Biotechniques, 32, 1372-1374, 1376, 1378-1379.
[20] Levy, G. and Degani, G. (2011) Evidence of a Reproduction-Related Function for Pituitary Adenylate Cyclase-Activating Polypeptide-Related Peptide in an Anabantidae Fish. Journal of Molecular Endocrinology, 46, 101-110.
[21] Degani, G. (1989) The Effect of Temperature, Light, Fish Size and Container Size on Breeding of Trichogaster trichopterus. Israeli Journal of Aquaculture Bamidgeh, 41, 67-73.
[22] Jackson, K., Degani, G. and Goldberg, D. (2004) Transcription of FSH-b, LH-b and Growth Hormone in Female Trichogaster trichopterus (Pallas 1770) Treated with GnRH Analog. IV International Congress on the Biology of Fish, Manaus, 2004, 45-57.
[23] Yomdin, G.D.S. (2011) Hormones Transcription in the Testis of Blue Gourami (Trichogaster trichopterus, Pallas 1770} During Sexual Behavier te during Sexual Behavior r Trichopterus, Pallas 1770). Bulletin UASVM Animal Science and Biotechnologies, 43-50.
[24] Degani, G., Jackson, K., Goldberg, D., Sarfati, R. and Avtalion, R.R. (2003) βFSH, βLH and Growth Hormone Gene Expression in Blue Gourami (Trichogaster trichopterus, Pallas 1770) during Spermatogenesis and Male Sexual Behavior. Zoological Science, 20, 737-743.

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