The Toxic Effects of Crude Oil in Some Freshwater Cyanobacteria


Toxicity effect of the Iraqi crude oil on the growth of two Cyanobacteria strains Microcystis flos-aquae (Wittr.) Kircher (unicellular) and Nostoc carneum Agardh (filamentous) was studied basing on it is biomass expressed as chlorophyll-a (μg/ml). Growth rate and doubling time of the cells were also calculated accordingly in present or absent of the crude oil. Also, Microscopical examination was observed to detect the morphological changes. Both species appeared different responses towards oil toxic influences with different concentrations and time of exposure. Growth was decreased gradually with Microcystis with addition of 10, 30, 50 and 70 mg/l of the crude oil, slightly toxic effects were observed with Nostoc in comparison to the control. Different significant correlations (P ≤ 0.05) were observed between growth of the cyanobacteria and the oil concentrations. Furthermore, results demonstrated that Microcystis was considered to be more sensitive than Nostoc towards crude oil concentrations.

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Obaidy, A. and Lami, M. (2014) The Toxic Effects of Crude Oil in Some Freshwater Cyanobacteria. Journal of Environmental Protection, 5, 359-367. doi: 10.4236/jep.2014.55039.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Sullivan, M.J. and Currin, C.A. (2000) Community Structure and Functional Dynamics of Benthic Microalgae in Salt Marshes. In: Weinstein, M.P. and Kreeger, D.A, Eds., Concepts and Controversies in Tidal Marsh Ecology, Kluwer Academic Publishers, Dordrecht, 81-106.
[2] Gouveia, L. (2011) Microalgae as a Feedstock for Biofuels. Springer Briefs in Microbiology.
[3] NRC (1985) Oil in the Sea-Inputs, Fates and Effects (1985) National Research Council. National Academy Press, Washington DC, 43-88.
[4] Tarshis, I.B. and Rattner, B.A. (1982) Accumulation of C14-naphthalene in the Tissues of Redhead Ducks Fed Oil-Contaminated Crayfish. Archive of Environmental Contamination and Toxicology, 11, 155-159.
[5] Vaajasaari, K., Joutti, A., Schultz, E., Selonen, S. and Westerholm, H. (2002) Comparisons of Terrestrial and Aquatic Bioassays for Oil-Contaminated Soil Toxicity. Journal of Soils and Sediments, 2, 194-202.
[6] Booth, L., Heppelthwaite, V. and O’Halloran, K. (2005) Effects-Based Assays in the Earthworm Aporrectodea caliginosa: Their Utilisation for Evaluation of Contaminated Sites before and after Remediation. Journal of Soils and Sediments, 5, 87-94.
[7] Grote, M., Schuurmann, G. and Altenburger, R. (2005) Modeling Photoinduced Algal Toxicity of Polycyclic Aromatic Hydrocarbons. Environmental Science and Technology, 39, 4141-4149.
[8] Ostgaard, K. (1994) The Oil, the Water and the Phytoplankton. Advances in Limnology, No. 42, 167-193.
[9] Ito, T., Tanaka, M., Shinkawa, H., Nakada, T., Ano, Y., Kurano, N., Soga, T. and Tomita, M. (2013) Metabolic and Morphological Changes of an Oil Accumulating Trebuoxiophycean Alga in Nitrogen-Deficient Conditions. Metabolomics, 9, 178-187.
[10] Gaur, J.P. and Kumar, H.D. (1981) Growth Response of Four Micro-algae to Three Crude Oils and a Furnace Oil. Environmental Pollution, 25, 77-85.
[11] Greca, M.D., Monaco, P., Pollio, A. and Previtera, L. (1992) Structure Activity Relationships of Phenylpropanoids as Growth Inhibitors of the Green Alga Selenastrum capricornutum. Phytochemistery, 31, 4119-4123.
[12] Chronopoulou, P.M., Fahy, A., Coulon, F., Passe, S., Goni-Urriza, M., Peperzak, L., Alvares, L.A., McKew, B.A., Lawson, T., Timmis, K.N., Duran, R., Underwood, G.J.C. and McGenity, T.J. (2013) Impact of a Simulated Oil Spill on Benthic Phototrophs and Nitrogen-Fixing Bacteria in Mudflat Mesocosms . Environmental Microbiology, 15, 242-252.
[13] Mohammady, N.G., Chen, Y. C., El-Mahdy, A.A. and Mohammad, R.F. (2005) Physiological Responses of the Eustigmatophycean Nannochloropsis salina to Aqueous Diesel Fuel Pollution. Oceanologia, 47, 75-92.
[14] Prince, R.C. (2010) Eukaryotic Hydrocarbon Degraders. In: Timmis, K.N., McGenity, T.J., van der Meer, J.R. and Lorenzo, V., Eds., Handbook of Hydrocarbon and Lipid Microbiology, Springer, Berlin, 2065-2078.
[15] Duran, R. and Goni-Urriza, M.S. (2010) Impact of Pollution on Microbial Mats. In: Timmis, K.N., McGenity, T.J., van der Meer, J.R. and Lorenzo, V., Eds., Handbook of Hydrocarbon and Lipid Microbiology, Springer, Berlin, 2339-2348.
[16] McGenity, T.J., Folwell, B.D., Mckew, B.A. and Sanni, G.O. (2012) Marine Crude-Oil Biodegradation: A Central Role for Interspecies Interactions. Aquatic Biosystems, 8, 10.
[17] Volkering, F., Breure, A.M., Sterkenburg, A. and van Andel, J.G. (1992) Microbial Degradation of Polycyclic Aromatic Hydrocarbons: Effect of Substrate Availability on Bacterial Growth Kinetics. Applied Microbiology and Biotechnology, 36, 548-552.
[18] Wodzinski, R.S. and Coyle, J.E. (1974) Physical State of Phenanthrene for Utilization by Bacteria. Applied and Environmental Microbiology, 27, 1081-1084.
[19] Sikkema, J., de Bont, J.A. and Poolman, B. (1995) Mechanisms of Membrane Toxicity of Hydrocarbons. Microbiology and Molecular Biology Reviews, 59, 201-222.
[20] Wang, Q., Zhang, S., Li, Y. and Klassen, W. (2011) Potential Approaches to Improving Biodegradation of Hydrocarbons for Bioremediation of Crude Oil Pollution. Journal of Environmental Protection, 2, 47-55.
[21] Chu, S.P. (1942) The Influence of the Mineral Composition of the Medium on the Growth of Planktonic Algae: Part I. Methods and Culture Media. The Journal of Ecology, 30, 284-325.
[22] Patterson, G. (1983) Effect of Heavy Metals on Fresh Water Chlorophyta. Ph.D. Thesis, Durham University, England.
[23] Karydis, M. (1979) Short Term Effects of Hydrocarbons on the Photosynthesis and Respiration of Some Phytoplankton Species. Botanica Marina, 22, 281-286.
[24] Vollenweider, R.A. (1974) A Manual on Methods for Measuring Primary Productivity in Aquatic Environments. 2nd Edition, IBP Handbook, No. 12, Blackwell Scientific Publication, Oxford.
[25] Reynolds, C.S. (1984) The Ecology of Freshwater Phytoplankton. Cambridge University Press, Cambridge.
[26] APHA, WWA and WEF (2005) Standard Methods for Examination of Water and Waste Water. 21st Edition, American Public Health Association, Washington DC.
[27] Agarwal, B.L. (1988) Basic Statistics. Wiley Eastern Ltd., New Delhi, 748.
[28] Oberholster, P.J., Blaise, C. and Botha, A.M. (2010) Phytobenthos and Phytoplankton Community Changes upon Exposure to Sunflower Oil Spill in a South African Protected Freshwater Wetland. Ecotoxicology, 19, 1426-1439.
[29] Margesin, R., Labbe, D., Schinner, F., Greer, C.W. and Whyte, L.G. (2003) Characterization of Hydrocarbon-Degrading Microbial Populations in Contaminated and Pristine Alpine Soils. Applied and Environmental Microbiology, 69, 3085-3092.
[30] De Oteyza, T.G., Grimalt, J.O., Diestra, E., Sole, T. and Esteve, I. (2004) Changes in the Composition of the Polar and Apolar Crude Oil Fractions under the Action of Microcoleus Consortia. Applied Microbiology and Biotechnology, 66, 226-232.
[31] Kusk, K.O. (1978) Effects of Crude Oil and Aromatic Hydrocarbons on the Photosynthesis of the Diatom Nitzschia palea. Physiologia Plantarum, 43, 1-6.
[32] Miller, M.C., Alexander, V. and Barsdate, R.J. (1978) The Effects of Oil Spills on Phytoplankton in an Arctic Lake and Ponds. Arctic, 31, 192-218.
[33] Miller, M.C., Hater, G.R. and Vestal, J.R. (1978) Effect of Prudhoe Crude Oil on Carbon Assimilation by Planktonic Algae in an Arctic Pond. In: Adrino, D.C. and Brisbin, I.L., Eds., Environmental Chemistry and Cycling Processes, Conference 760429, US Department of Energy, Washington DC, 833-850.
[34] Tukaj, Z. (1987) The Effects of Crude and Fuel Oils on the Growth, Chlorophyll ‘a’ Content and Dry Matter Production of a Green Alga Scenedesmus Quadricauda (Turp.) Bréb. Environmental Pollution, 47, 9-24.
[35] Van Putte, R.D. and Patterson, C.O. (1995) Isolation and Purification of Plasma Membranes form Three Species of Marine Microalgae. Proceeding of Phycolgical Society of America, Breckenridge, 31, 8.
[36] Nechev, J.T., Khotimchenko, S.V., Ivanova, A.P., Stefanov, K.L., Dimitrova-Konaklieva, S.D., Andreev, S. and Popov, S.S. (2002) Effect of Diesel Fuel Pollution on the Lipid Composition of Some Wide-Spread Black Sea Algae and Invertebrates. Zeitschrift für Naturforschung. C, 57, 339-343.
[37] Soto, C., Hellebust, J.A. and Hutchinson, T.C. (1977) Effect of Naphthalene and Aqueous Crude Oil Extracts on the Green Flagellate Chlamydomonas angulosa. III. Changes in Cellular Composition. Canadian Journal of Botany, 55, 2765-2777.
[38] Zachleder, V. and Setlik, I. (1982) Effect of Irradiance on the Course of RNA Synthesis in the Cell Cycle of Scenedesmus quadricauda. Biologia Plantarum, 24, 341-353.
[39] Al Hasan, R.H., Sorkhoh, N.A., Al-Bader, D. and Radwan, S.S. (1994) Utilization of Hydrocarbons by Cyanobacteria from Microbial Mats on Oily Coasts of the Gulf. Applied Microbiology and Biotechnology, 41, 615-619.
[40] Gamila, H.A. and Ibrahim, B.M. (2004) Algal Bioassay for Evaluating the Role of Algae in Bioremediation of Crude Oil: I. Isolated Strains. Bulletin of Environmental Contamination and Toxicology, 73, 883-889.
[41] Dunstan, W.M., Atkinson, L.P. and Natoli, J. (1975) Stimulation and Inhibition of Phytoplankton Growth by Low Molecular Weight Hydrocarbons. Marine Biology, 31, 305-310.
[42] Petkov, G.D., Furnadzieva, S.D. and Popov, S.S. (1992) Petrol-Induced Changes in the Lipid and Sterol Composition of Three Microalgae. Phytochemistry, 31, 1165-1166.
[43] Cerniglia, C.E. (1984) Microbial Metabolism of Polycyclic Aromatic Hydrocarbons. Advances in Applied Microbiology, 30, 31-71.
[44] Ibrahim, M.B.M. and Gamila, H.A. (2004) Algal Bioassay for Evaluating the Role of Algae in Bioremediation of Crude Oil: II. Freshwater Phytoplankton Assemblages. Bulletin of Environmental Contamination and Toxicology, 73, 971-978.
[45] Karydis, M. (1981) The Toxicity of Crude Oil for the Marine Alga Skeletonema costatum (Greville) Cleve in Relation to Nutrient Limitation. Hydrobiologia, 85, 137-143.
[46] Soto, C., Hutchinson, T.C., Hellebust, J.A. and Sheath, R.G. (1979) The Effect of Crude Oil on the Morphology of the Green Flagellate Chlamydomonas angulosa. Canadian Journal of Botany, 57, 2717-2728.
[47] Tukaj, Z., Kentzer, T. and Bohdanowicz, J. (1984) The Influence of Fuel Oil on the Growth and Cell Morphology of Scenedesmus quadricauda (Turp.) Breb. Proceeding of 14 Conference of Baltic Oceanographers, 2, 830-844.
[48] Gaur, J.P. and Singh, A.K. (1990) Growth, Photosynthesis and Nitrogen Fixation of Anabaena doliolum Exposed to Assam Crude Extract. Bulletin of Environmental Contamination and Toxicology, 44, 494-500.
[49] Gamila, H.A., Ibrahim, M.B.M. and Abd El-Gafar, H.H. (2003) The Role of Cyanobacterial Isolated Strains in the Biodegradation of Crude Oil. International Journal of Environmental Studies, 60, 435-444.

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