Effects of Bradyrhizobium japonicum Inoculation and Supplementation with Phosphorus on Macronutrients Uptake in Cowpea (Vigna unguiculata (L.) Walp)


The current study was conducted to assess the effects of phosphorus supplementation and Bradyrhizobium japonicum inoculation on the availability and uptake of N, P, K, Mg, Ca and Na on cowpea. The experiment was laid out in a split plot design where the main plots comprised two inoculation levels (with and without inoculation of B. japonicum ) and sub plots contained four different levels of phosphorus (0, 20, 40, and 80 kg P/ha). The results indicated that B. japonicum inoculation and phosphorus supplementation significantly improved the uptake of N, P, K, Mg, Ca and Na in different cowpea tissues such as roots, pods, shoots, and whole plant relative to the control. The results also indicated that there was a significant interaction of B. japonicum and phosphorus on the uptake of sodium in the roots and whole cowpea plant in the screen house. Rhizobia inoculation and supplementation of phosphorus at 40 kg P/ha resulted in the improved uptake of the most elements over other treatments tested.

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D. Nyoki and P. Ndakidemi, "Effects of Bradyrhizobium japonicum Inoculation and Supplementation with Phosphorus on Macronutrients Uptake in Cowpea (Vigna unguiculata (L.) Walp)," American Journal of Plant Sciences, Vol. 5 No. 4, 2014, pp. 442-451. doi: 10.4236/ajps.2014.54058.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] P. A. Ndakidemi, S. Bambara and J. H. J. R. Makoi, “Micronutrient Uptake in Common Bean (“Phaseolus vulgaris” L.) as Affected by Rhizobium Inoculation, and the Supply of Molybdenum and Lime,” Plant Omics, Vol. 4, No. 1, 2011, pp. 40-52.
[2] R. Uchida, “Essential Nutrients for Plant Growth: Nutrient Functions and Deficiency Symptoms, Plant Nutrient Management in Hawaii’s Soils,” College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, 2000, pp. 31-55.
[3] J. H. J. R. Makoi, S. Bambara and P. A. Ndakidemi, “Rhizobium Inoculation and the Supply of Molybdenum and Lime Affect the Uptake of Macroelements in Common Bean (P. vulgaris L.) Plants,” Australian Journal of Crop Science, Vol. 7, No. 6, 2013, pp. 784-794.
[4] I. Cechin and T. de F. Fumis, “Effect of Nitrogen Supply on Growth and Photosynthesis of Sunflower Plants Grown in the Greenhouse,” Plant Science, Vol. 166, No. 5, 2004, pp. 1379-1385.
[5] B. Saharan and V. Nehra, “Plant Growth Promoting Rhizobacteria: A Critical Review,” Life Sciences and Medical Research, Vol. 2011, LSMR-21, 2011, pp. 1-30.
[6] V. N. Matiru and F. D. Dakora, “Potential Use of Rhizobial Bacteria as Promoters of Plant Growth for Increased Yield in Landraces of African Cereal Crops,” African Journal of Biotechnology, Vol. 3, No. 1, 2004, pp. 1-7.
[7] C. Tang, P. Hinsinger, J. J. Drevon and B. Jaillard, “Phosphorus Deficiency Impairs Early Nodule Functioning and Enhances Proton Release in Roots of Medicago truncatula L,” Annals of Botany, Vol. 88, No. 1, 2001, pp. 131-138. http://dx.doi.org/10.1006/anbo.2001.1440
[8] A. Singh, A. Baoule, H. Ahmed, A. Dikko, U. Aliyu, M. Sokoto, J. Alhassan, M. Musa and B. Haliru, “Influence of Phosphorus on the Performance of Cowpea (Vigna unguiculata (L) Walp.) Varieties in the Sudan Savanna of Nigeria,” Agricultural Sciences, Vol. 2, No. 3, 2011, pp. 313-317. http://dx.doi.org/10.4236/as.2011.23042
[9] M. O. Akande, “Effect of Phosphate Rock on Selected Chemical Properties and Nutrient Uptake of Maize and Cowpea Grown Sequentially on Three Soil Types in South Western Nigeria,” International Research Journal of Agricultural Science and Soil Science, Vol. 1, No. 11, 2011, pp. 471-480.
[10] A. Hussain, A. Ali and I. R. Noorka, “Effect of Phosphorus with and without Rhizobium Inoculation in Nitrogen and Phosphorus Concentration and Uptake by Mungbean (Vigna radiata L),” Journal of Agricultural Research, Vol. 50, No. 1, 2012, pp. 49-57.
[11] R. J. Buresh, P. A. Sanchez and F. Calhoun, “Replenishing Soil Fertility in Africa,” SSSA Special Publication No. 51, Soil Science Society of America, Madison, Wisconsin, 1997, p. 251.
[12] J. M. Bremner, “Total Nitrogen,” In: C. A. Black, Ed., Methods of Soil Analysis, Part 2, American Society of Agronomy, Madison, 1965, pp. 1149-1178.
[13] J. Murphy and J. P. Riley, “A Modified Single Solution Method for the Determination of Phosphate in Natural Water,” Analytica Chimica Acta, Vol. 27, 1962, pp. 31-36.
[14] P. R. Hesse, “A Textbook of Soil Chemical Analysis,” Murray, London, 1971, pp. 120-309.
[15] R. G. D. Steel and J. H. Torrie, “Principles and Procedures of Statistics: A Biometrical Approach,” 2nd Edition, McGraw-Hill Kogakusha, New York, 1980.
[16] S. B. G. Christoph, W. G. Teixeira, J. Lehmann, W. E. H. Blum and W. Zech, “Nitrogen Retention and Plant Uptake on a Highly Weathered Central Amazonian Ferralsol Amended with Compost and Charcoal,” Journal of Plant Nutrition and Soil Sciences, Vol. 171, No. 6, 2008, pp. 893-899. http://dx.doi.org/10.1002/jpln.200625199
[17] J. O. Achieng and G. Odhiambo, “Use of Organic Inputs in Management of Alfisols and Ultisols for Sustainable Maize Production in Western Kenya,” American Journal of Experimental Agriculture, Vol. 3, No. 4, 2013, pp. 884-895.
[18] A. Bationo, B. Ntare, S. Tarawali and R. Tabo, “Soil Fertility Management and Cowpea Production in the Semiarid Tropics,” Challenges and Opportunities for Enhancing Sustainable Cowpea Production, 2002, pp. 301-318.
[19] M. F. Baqual and P. K. Das, “Influence of Biofertilizers on Macronutrient Uptake by the Mulberry Plant and Its Impact on Silkworm Bioassay,” Caspian Journal of Environmental Sciences, Vol. 4, No. 2, 2006, pp. 98-109.
[20] S. Bambara and P. A. Ndakidemi, “Changes in Selected Soil Chemical Properties in the Rhizosphere of Phaseolus vulgaris L. Supplied with Rhizobium Inoculants, Molybdenum and Lime,” Scientific Research and Essays, Vol. 5, No. 7, 2010, pp. 679-684.
[21] M. Bagayoko, A. Buerkert, G. Lung, A. Bationo and V. Romheld, “Cereal/Legume Rotation Effects on Cereal Growth in Sudano-Sahelian West Africa: Soil Mineral Nitrogen, Mycorrhizae and Nematodes,” Plant and Soil, Vol. 218, No. 1-2, 2000, pp. 103-116.
[22] M. H. Abd-alla, “Phosphatases and the Utilization of Organic P by Rhizobium leguminosarum Biovarviceae,” Letters in Applied Microbiology, Vol. 18, No. 5, 1994, pp. 294-296.
[23] A. Halder and P. K. Chakrabartty, “Solubilization of Inorganic Phosphate by Rhizobium,” Folia Microbiologica, Vol. 38, No. 4, 1993, pp. 325-330.
[24] P. Singleton and J. Tavares, “Inoculation Response of Legumes in Relation to the Number and Effectiveness of Indigenous Rhizobium Populations,” Applied and Environmental Microbiology, Vol. 51, No. 5, 1986, pp. 1013-1018.
[25] I. Magani and C. Kuchinda, “Effect of Phosphorus Fertilizer on Growth, Yield and Crude Protein Content of Cowpea (Vigna unguiculata (L.) Walp) in Nigeria,” Journal of Applied Biosciences, Vol. 23, 2009, pp. 1387-1393.
[26] Y. C. Ann, “Determination of Nutrient Uptake Characteristic of Black Pepper (Piper nigrum L.),” Journal of Agricultural Science and Technology, Vol. 2, No. 10B, 2012, pp. 1091-1099.
[27] R. Hayat, S. Ali, U. Amara, R. Khalid and I. Ahmed, “Soil Beneficial Bacteria and Their Role in Plant Growth Promotion: A Review,” Annals of Microbiology, Vol. 60, No. 4, 2010, pp. 579-598.
[28] P. A. Ndakidemi, “Nutritional Characterization of the Rhizosphere of Symbiotic Cowpea and Maize Plants in Different Cropping Systems,” Ph.D. Thesis, Cape Peninsula University of Technology, Cape Town, 2005, p. 150.
[29] N. Stamford, C. Santos and S. Dias, “Phosphate Rock Biofertiliser with Acidithiobacillus and Rhizobia Improves Nodulation and Yield of Cowpea (Vigna unguiculata) in Greenhouse and Field Conditions,” Tropical Grasslands, Vol. 40, No. 4, 2006, pp. 222-230.
[30] D. Nyoki and P. A. Ndakidemi, “Economic Benefits of Bradyrhizobium japonicum Inoculation and Phosphorus Supplementation in Cowpea (Vigna unguiculata (L) Walp) Grown in Northern Tanzania,” American Journal of Research Communication, Vol. 1, No. 11, 2013, pp. 173-189.

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