Improvement of Medium Composition and Utilization of Mixotrophic Cultivation for Green and Blue Green Microalgae towards Biodiesel Production

DOI: 10.4236/aim.2014.43022   PDF   HTML     4,221 Downloads   6,590 Views   Citations


A possible source of biological material for the production of biodiesel is represented by microalgae, in particular by their lipid content. The aim of the present work was to optimize culture medium composition for improving growth and lipid content of green microalgae Chlorella sorokiniana, Scenedesmus acuminatus and blue green Cyanobacterium aponicum. Lipids were quantitatively determined by spectrofluorometric method using Nile red flurometric stain. Initially, the effect of two different medium types, Bolds and optimized culture medium (OCM), four types of carbon source (glucose and sodium acetate, molasses, glycerol, control) and four nitrogen concentrations (100%, -75%, -50%, -25%) on the enhancement of biomass and lipid content and lipid productivity were studied; indeed, optimized culture medium significantly improved growth, CDW for three microalgae, beside increasing lipid content and lipid productivity for S. acuminatus and C. aponicum by 7.5 and 5 folds respectively at 25th day compared to Bolds medium. Moreover, 25% nitrogen deficient medium significantly increased lipid content and lipid productivity for both C. sorokiniana and C. aponicum at 2nd week of re-propagation to 10.6 and 2.6 folds over control (100% nitrogen). While S. acuminatus recorded the significant lipid content & productivity at 2nd week under recommended nitrogen dose in medium (100% N) by 4.4 folds over 25% deficient medium. Meanwhile 0.3% glycerol medium enhanced CDW, lipid content of S. acuminatus to 1.68 gL-1. While C. sorokiniana and C. aponicum recorded significant CDW under 0.3% acetate medium 1.37 and 0.76 gL-1. C. aponicum exhibited no growth under glycerol medium. The highest lipid content and lipid productivity were obtained under glycerol medium for C. sorokiniana and S. acuminatus (64.3 and 52.8 mg·g·g-1·d-1).

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S. Hamed and G. Klöck, "Improvement of Medium Composition and Utilization of Mixotrophic Cultivation for Green and Blue Green Microalgae towards Biodiesel Production," Advances in Microbiology, Vol. 4 No. 3, 2014, pp. 167-174. doi: 10.4236/aim.2014.43022.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] J. Pruvost, G. Van Vooren, B. Le Gouic, A. CouzinetMossion and J. Legrand, “Systematic Investigation of Biomass and Lipid Productivity by Microalgae in Photobioreactors for Biodiesel Application,” Bioresource Technology, Vol. 102, No. 1, 2011, pp. 150-158.
[2] M. J. Griffiths and S. T. L. Harrison, “Lipid Productivity as a Key Characteristic for Choosing Algal Species for Biodiesel Production,” Journal of Applied Phycology, Vol. 21, No. 5, 2009, pp.493-507.
[3] Q. Hu, M. Sommerfeld, E. Jarvis, M. L. Ghirardi, M. Posewitz, M. Seibert and A. Darzins, “Microalgal Triacylglycerols as Feedstocks for Biofuel Production: Perspectives and Advances,” The Plant Journal for Cell and Molecular Biology, Vol. 54, No. 4, 2008, pp. 621-639.
[4] Y. Chisti, “Biodiesel from Microalgae,” Biotechnology Advances, Vol. 25, No. 3, 2007, pp. 294-306.
[5] L. Rodolfi, G. Chini Zittelli, N. Bassi, G. Padovani, N. Biondi, G. Bonini and M. Tredici, “Microalgae for Oil: Strain Selection, Induction of Lipid Synthesis and Outdoor Mass Cultivation in a Low-Cost Photobioreactor,” Biotechnology and Bioengineering, Vol. 102, No. 1, 2009, pp. 100-112.
[6] J. N. Rosenberg, G. A. Oyler, L. Wilkinson and M. J. Betenbaugh, “A Green Light for Engineered Algae: Redirecting Metabolism to Fuel a Biotechnology Revolution,” Current Opinion in Biotechnology, Vol. 19, No. 5, 2008, pp. 430-436.
[7] Y.-K. Lee, “Microalgal Mass Culture Systems and Methods: Their Limitation and Potential,” Journal of Applied Phycology, Vol. 13, No. 4, 2001, pp. 307-315.
[8] G. Yu, D. Shi, Z. Cai, W. Cong and F. Ouyang, “Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture,” Chinese Journal of Chemical Engineering, Vol. 19, No. 1, 2011, pp. 108-115.
[9] Y.-I. Yamane, T. Utsunomiya, M. Watanabe and K. Sasaki, “Biomass Production in Mixotrophic Culture of Euglena gracilis under Acidic Condition and Its Growth Energetic,” Biotechnology Letters, Vol. 23, No. 15, 2001, pp. 1223-1228.
[10] D. Yan, Y. Lu, Y.-F. Chen and Q. Wu, “Waste Molasses Alone Displaces Glucose-Based Medium for Microalgal Fermentation towards Cost-Saving Biodiesel Production,” Bioresource Technology, Vol. 102, No. 11, 2011, pp. 6487-6493.
[11] H. W. Bischoff and H. C. Bold, “Phycological Studies IV. Some Soil Algae from Enchanted Rock and Related Algal Species,” University of Texas Publication No. 6318, 1963, p. 95.
[12] T. Heredia-Arroyo, W. Wei, R. Ruan and B. Hu, “Mixotrophic Cultivation of Chlorella vulgaris and Its Potential Application for the Oil Accumulation from Non-Sugar Materials,” Biomass and Bioenrgy, Vol. 35, No. 5, 2011, pp. 2245-2253.
[13] E. Bertozzini, L. Galluzzi, A. Penna and M. Magnani, “Application of the Standard Addition Method for the Absolute Quantification of Neutral Lipids in Microalgae Using Nile Red,” Journal of Microbiological Methods, Vol. 87, No. 1, 2011, pp. 17-23.
[14] R. K. Mandalam and B. Ø. Palsson, “Cell Cycle of Chlorella vulgaris Can Deviate from the Synchronous Binary Division Model,” Biotechnology Letters, Vol. 19, No. 6, 1997, pp. 587-591.
[15] R. Huerlimann, R. de Nys and K. Heimann, “Growth, Lipid Content, Productivity, and Fatty Acid Composition of Tropical Microalgae for Scale-Up Production,” Biotechnology and Bioengineering, Vol. 107, No. 2, 2010, pp. 245-257.
[16] D. H. Turpin, “Effect of Inorganic N Availability on Algal Photosynthesis and Carbon Metabolism,” Journal of Phycology, Vol. 27, No. 1, 1991, pp. 14-20.
[17] F. J. L. Gordillo, C. Jimenez, F. L. Figueroa and F. X. Niel, “Effect of Increased Atmospheric CO2 and N Supply on Photosynthesis, Growth and Cell Composition of the Cyanobacterium Spirulina platensis,” Journal of Applied Phycology, Vol. 10, No. 5, 1998, pp. 461-469.
[18] M. Piorreck, K.-H. Baasch and P. Pohl, “Biomass Production, Total Protein, Chlorophylls, Lipids and Fatty Acids of Fresh Water Green and Blue-Green Algae under Different Nitrogen Regimes,” Phytochemistry, Vol. 23, No. 2, 1984, pp. 207-216.
[19] A. M. Illman, A. H. Scragg and S. W. Shales, “Increase in Chlorella Strains Calorific Values when Grown in Low Nitrogen Medium,” Enzyme and Microbial Technology, Vol. 27, No. 8, 2000, pp. 631-635.
[20] Z.-Y. Liu, G.-C. Wang and B.-C. Zhou, “Effect of Iron on Growth and Lipid Accumulation in Chlorella vulgaris,” Bioresource Technology, Vol. 99, No. 11, 2008, pp. 4717-4722.
[21] B. Richardson, D. M. Orcutt, H. A. Schwertner, L. Martinez and E. Wickline, “Effects of Nitrogen Limitation on the Growth and Composition of Unicellular Algae in Continuous Culture, ” Appllied and Environmental Micobiology, Vol. 18, No. 2, 1969, pp. 245-250.
[22] M. El-Sheekh, A. Abomohra and D. Hanelt, “Optimization of Biomass and Fatty Acid Productivity of Scenedesmus obliquus as a Promising Microalga for Biodiesel Production,” World Journal of Microbiology Biotechnology, Vol. 29, No. 5, 2012, pp. 915-922.
[23] A. P. Ivanova, R. R. Robaina, J. Martin and K. L. Stefanov, “Effect of Glycerol on the Lipids in the Red Alga Gratelupia doryphora,” Grasas Aceites, Vol. 50, No. 6, 1999, pp. 469-471.
[24] é. C. Francisco, D. B. Neves, E. Jacob-Lopes and T. T. Franco, “Microalgae as Feedstock for Biodiesel Production: Carbon Dioxide Sequestration, Lipid Production and Biofuel Quality,” Journal of Chemical Technology and Biotechnology, Vol. 85, No. 3, 2010, pp. 395-403.
[25] M. E. Huntley and D. G. Redalje, “CO2 Mitigtion and Renewable Oil from Photosynthetic Microbes,” Mitigation and Adaptation Strategies for Global Change, Vol. 12, No. 4, 2007, pp. 573-608.
[26] A. Widjaja, C.-C. Chein and Y.-H. Ju, “Study of Increasing Lipid Production from Fresh Water Microalgae Chlorella vulgaris,” Journal of the Taiwan Institute of Chemical Engineers, Vol. 40, No. 1, 2009, pp. 13-20.
[27] W. Crueger and A. Crueger, “Biotecnologia: Manual de Microbiologia Industrial,” Zaragoza Acribia S.A., 1989.

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