Horseradish Peroxidase Biosensor to Detect Zinc Ions in Aqueous Solutions

Mambo Moyo

Department of Chemical Technology, Midlands State University, Gweru, Zimbabwe.

DOI: 10.4236/ojab.2014.31001                

Abstract

Maize tassel-multiwalled carbon nanotube (MT-MWCNT) composite has been used as a matrix for physical adsorption of horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The HRP/MT-MWCNT biosensor was applied for the detection of Zn²⁺ in aqueous solution. The biosensor designed was able to determine Zn²⁺ in the range of 0.35 - 12 mg/L with a detection limit of 7.5 μg/L. The inhibition was found to be reversible and uncompetitive when data were modeled using the Dixon and Cornish-Bowden plots. The biosensor was found to have good repeatability, reproducibility and high selectivity. The developed biosensor can be used to detect other HRP inhibiting trace metal ions.

Keywords

Horseradish Peroxidase; Maize Tassel; Multiwalled Carbon Nanotubes; Metal Biosensor; Zn²⁺

Conflicts of Interest

The authors declare no conflicts of interest.

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