Intramedullary Spinal Cord Glioma Following Microinjection of Glioblastoma Cell Line C6 in Rats

Yasar Dagistan, Gulzade Ozyalvacli, Tulin Firat, Kaan Yagmurlu, Elcin Hakan Terzi

1st Neurosurgery Clinic in Bakirkoy Mental Diseases Hospital, Bakirkoy, Istanbul, Turkey.
Department of Histology and Embryology, Abant Izzet Baysal University, Medical School, Bolu, Turkey.
Department of Neurosurgery, Abant Izzet Baysal University, Medical School, Bolu, Turkey.
Department of Pathology, Abant Izzet Baysal University, Medical School, Bolu, Turkey.

DOI: 10.4236/ojmn.2014.41010                

Abstract

Background: This paper describes the establishment of a rat intramedullary spinal cord tumor (IMSCT) model and histopathological characterization of the tumor model. Methods: Fourteen male Wistar rats were randomized into two groups. The rats in group 1 (control group, n = 7) received a 5 μl intramedullary injection of serum physiologic (SF). Those in group 2 (experimental group, n= 7) received a 5 μl intramedullary implantation of media containing 5 × 10⁵ C6 glioma cells. The animals were sacrificed for histopathological examination at 21 days. Results: The control group showed normal functional and histopathological findings. The group 2 rats implanted with C6 glioblastoma cells developed hind-limb paraplegia. Pathological sections confirmed intramedullary C6 glioblastoma invading the spinal cord. Conclusions: A rat C6 IMSCT model was successfully established. This model may be useful in increasing understanding of intramedullary spinal cord gliomas in humans.

Keywords

Intramedullary Spinal Cord Glioma; Animal Model; C6 Glioblastoma Cell

Conflicts of Interest

The authors declare no conflicts of interest.

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