A method to synthesize cDNA constructs by homology based recombination cloning

Abstract

We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB1 and MYB2 genes of Arabidopsis thaliana.

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Verma, N. and Burma, P. (2014) A method to synthesize cDNA constructs by homology based recombination cloning. American Journal of Molecular Biology, 4, 16-19. doi: 10.4236/ajmb.2014.41003.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Sleight, S.C., Bartley, B.A., Lieviant, J.A. and Sauro, H.M. (2010) In-fusion biobrick assembly and re-engineering. Nucleic Acids Research, 38, 2624-2636.
http://dx.doi.org/10.1093/nar/gkq179
[2] Topfer, R., Matzeit, V., Bruno, G., Schell, J. and Steinbiss, H.H. (1987) A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Research, 15, 5890.

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