Ochratoxin A in dried figs, raisings, apricots, dates on Iranian retail market

Abstract

Ochratoxin A (OTA) is among the most important mycotoxins, and the International Agency for Research on Cancer (IARC) classifies it as possibly carcinogenic to humans (group 2B). A total of 121 samples of dried fruits from the central zone of Iran were analyzed for OTA by enzyme linked immunosorbent assay (ELISA) technique. The recovery percentages of OTA in spiked dried fruit samples at concentrations of 5, 10 and 20 ng/g were found to be 84.9%, 89.3% and 90.4% as mean, respectively. OTA was found in 20.7% of the analyzed samples by average concentration of 6.7 ± 3.9 ng/g. The incidence rates of OTA contamination in dried fig, raising, apricots, and date samples were 10.4%, 44.7%, 6.7% and 10.0%, respectively. The concentrations of OTA in 7.9% of contaminated dried raising samples and 2.1% of dried fig samples were higher than maximum tolerance limit accepted by European Union (10 ng/g). This value reflects that the analyzed samples have a minimal contribution to toxicological risk. To our best knowledge, the present study is the first report of the presence of OTA by ELISA in dried fruit samples in Iran.

 

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Rahimi, E. and Shakerian, A. (2013) Ochratoxin A in dried figs, raisings, apricots, dates on Iranian retail market. Health, 5, 2077-2080. doi: 10.4236/health.2013.512282.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Pfohl-Leszkowicz, A. and Manderville, R.A. (2007) Ochratoxin A: an overview on toxicity and carcinogenicity in animals and humans. Molecular Nutrition and Food Research, 51, 61-99.
http://dx.doi.org/10.1002/mnfr.200600137
[2] Medina, C., Mateo, R., López-Ocana, L., Valle-Algarra, F.M. and Jiménez, M. (2005) Study on Spanish grape mycobiota and ochratoxin A production by isolates of Aspergillus tubingensis and other members of Aspergillus section Nigri. Applied and Environmental Microbiology, 71, 4696-4702.
http://dx.doi.org/10.1128/AEM.71.8.4696-4702.2005
[3] Perrone, G., Mulè, G., Susca, A., Battilani, P., Pietri, A. and Logrieco, A. (2006) Ochratoxin A production and amplified fragment length polymorphism analysis of Aspergillus carbonarius, Aspergillus tubingensis, and Aspergillus niger strains isolated from grapes in Italy. Applied and Environmental Microbiology, 72, 680-685.
http://dx.doi.org/10.1128/AEM.72.1.680-685.2006
[4] Bellí, N., Ramos, A.J., Coronas, I., Sanchis, V. and Marín, S. (2005) Aspergillus carbonarius growth and ochratoxin A production on a synthetic grape medium in relation to environmental factors. Journal of Applied Microbiology, 98, 839-844.
http://dx.doi.org/10.1111/j.1365-2672.2004.02469.x
[5] Pardo, E., Sanchis, V., Ramos, A.J. and Marin, S. (2006) Non-specificity of nutritional substrate for ochratoxin A production by isolates of Aspergillus ochraceus. Food Microbiology, 23, 351-358.
http://dx.doi.org/10.1016/j.fm.2005.05.005
[6] Goryacheva, I.Y., De Saeger, S., Lobeau, M., Eremin, S.A., Barna-Vetro, I. and Van P.C. (2006) Approach for ochratoxin A fast screening in spices using clean-up tandem immunoassay columns with confirmation by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Analytica Chimica Acta, 577, 38-45. http://dx.doi.org/10.1016/j.aca.2006.06.033
[7] Ghali, R., Hmaissia-khlifa, K., Ghorbel, H., Maaroufi, K. and Hedili, A. (2008) Incidence of aflatoxins, ochratoxin A and zearalenone in Tunisian foods. Food Control, 19, 921-924.
http://dx.doi.org/10.1016/j.foodcont.2007.09.003
[8] Peraica, M., Flajs, D., Domijan, A.M., Ivic, D. and Cvjetkovic, B. (2010) Ochratoxin A Contamination of Food from Croatia. Toxins, 2, 2098-2105.
http://dx.doi.org/10.3390/toxins2082098
[9] Sava, V., Reunova, O., Velasquez, A., Harbison, R. and Sanchez-Ramos, J. (2006) Acute neurotoxic effects of the fungal metabolite ochratoxin-A. Neurotoxicology, 27, 82-92. http://dx.doi.org/10.1016/j.neuro.2005.07.004
[10] International Agency for Research on Cancer (1993) Some naturally occurring substances: Food items and constituents, heterocyclic aromatic amines and mycotoxins. Monographs on the Evaluation of Carcinogenic Risks to Humans, 56, 489-521.
[11] EFSA (European Food Safety Authority) (2006) Opinion of the scientific panel on contaminants in the food chain on a request from the commission related to ochratoxin A in food. Adopted on 4 April 2006. The European Food Safety Authority Journal, 365, 1-56.
[12] European Commission Regulation (2006) Setting maximum levels for certain contaminants in foodstuffs. No. 1881/2006 of 19 December 2006. Official Journal of the European Union, 364, 5-24.
[13] Ghali R., Hmaissia-khlifa K., Ghorbel H., Maaroufi K. and Hedili A. (2009) HPLC determination of ochratoxin A in high consumption Tunisian foods. Food Control, 20, 716-720.
http://dx.doi.org/10.1016/j.foodcont.2008.09.004
[14] Food and Agriculture Organization (2004) Worldwide regulations for mycotoxins in food and feed in 2003. FAO Food and Nutrition Paper No. 81. Rome. FAO.
[15] Bircan, C. (2009) Incidence of ochratoxin A in dried fruits and co-occurrence with aflatoxins in dried figs. Food and Chemical Toxicology, 47, 1996-2001.
http://dx.doi.org/10.1016/j.fct.2009.05.008
[16] Senyuva, H.Z., Gilbert, J., Ozcan, S. and Ulken, U. (2005) Survey for co-occurrence of ochratoxin A and aflatoxin B1 in dried figs in Turkey by using a single laboratory-validated alkaline extraction method for ochratoxin A. Journal of Food Protection, 68, 1512-1515.
[17] Ozay, G. and Alperden, F. (1991) Aflatoxin and ochratoxin A contamination of dried figs (Ficus carina L) form the 1988 crop. Mycotoxin Research, 7, 85-91.
http://dx.doi.org/10.1007/BF03192171
[18] Bayman, P., Baker, J.L., Doster, M.A., Michailides, T.J. and Mahoney, N.E. (2002) Ochratoxin production by the Aspergillus ochraceus group and Aspergillus alliaceus. Applied and Environmental Microbiology, 68, 2326-2329.
http://dx.doi.org/10.1128/AEM.68.5.2326-2329.2002
[19] Guler, F.K. and Heperkan, D. (2008) Natural occurrence of ochratoxin A in dried figs. Analytica Chimica Acta, 617, 32-36. http://dx.doi.org/10.1016/j.aca.2008.01.009
[20] Zinedine, A., Soriano, J.M., Juan, C., Mojemmi, B., Molto, J.C., Bouklouze, A., Cherrah, Y., Idrissi, L., El Aouad, R. and Manes, J. (2007). Incidence of ochratoxin A in rice and dried fruits from Rabat and Sale area, Morocco. Food Additives and Contaminants, 24, 285-291.
http://dx.doi.org/10.1080/02652030600967230
[21] Lamanaka, B.T., Taniwaki, M.H., Menezes, H.C., Vicente, E. and Fungaro, M.H.P. (2005) Incidence of toxigenic fungi and ochratoxin A in dried fruits sold in Brazil. Food Additives and Contaminants, 22, 1258-1263.
http://dx.doi.org/10.1080/02652030500260447
[22] Varga, J., Kocsube, S., Koncz, Z. and Teren, J. (2006) Mycobiota and ochratoxin Ain raisins purchased in Hungary. Acta Alimentaria, 35, 289-294.
http://dx.doi.org/10.1556/AAlim.35.2006.3.6
[23] Trucksess, M.W. and Scott, P.M. (2008) Mycotoxins in botanicals and dried fruits: A review. Food Additives and Contaminants, 25, 181-192.
http://dx.doi.org/10.1080/02652030701567459
[24] Magnoli, C., Astoreca, A., Ponsone, L., Combina, M., Palacio, G., Rosa, C.A. and Dalcero, A.M. (2004) Survey of mycoflora and ochratoxin A in dried vine fruits from Argentina markets. Letters in Applied Microbiology, 39, 326-331.
http://dx.doi.org/10.1111/j.1472-765X.2004.01583.x
[25] Imperato, R., Campone, L., Piccinelli, A.L., Veneziano, A. and Rastrelli, L. (2011) Survey of aflatoxins and ochratoxin a contamination in food products imported in Italy. Food Control, 22, 1905-1910.
http://dx.doi.org/10.1016/j.foodcont.2011.05.002
[26] Engel, G. (2000) Ochratoxin A in sweets, oil seeds and dairy products. Archive für Lebensmittelhygiene, 51, 98-101.
[27] Mouller, T. E., Nyberg, M. (2003) Ochratoxin A in raisins and currants: Basic extraction procedure used in two small marketing surveys of the occurrence and control of the heterogeneity of the toxins in samples. Food Additives and Contaminants, 20, 1072-1076.
http://dx.doi.org/10.1080/02652030310001615212
[28] Lombaert, G.A., Pellaers, P., Neumann, G., Kitchen, D., Huzel, V., Trelka, R., Kotello, S. and Scott, P.M. (2004) Ochratoxin A in dried vine fruits on the Canadian retail market. Food Additives and Contaminants, 21, 578-585.
http://dx.doi.org/10.1080/02652030410001687681
[29] Youssef, M.S., Abo-Dahab, N.F. and Abou-Seidah, A.A. (2000) Mycobiota and mycotoxin contamination of dried raisins in Egypt. African Journal of Mycology and Biotechnology, 8, 69-86.
[30] Aksoy, U., Dunbay, O. and Gulseri, O. (1995) Survey of aflatoxins and ochratoxin A in Turkish dried apricots. ISHS Acta Horticulturae, 384, 651-654.
[31] Pietri, A., Bertuzzi, T., Pallaroni, L. and Piva, G. (2001) Occurrence of ochratoxin A in Italian wines. Food Additives and Contaminants, 18, 647-654.
[32] Lopez de Cerain, A., Gonzáles-Penas, E., Jiménez, A.M. and Bello, J. (2002) Contribution to the study of ochratoxin A in Spanish wines. Food Additives and Contaminants, 19, 1058-1064.
http://dx.doi.org/10.1080/02652030210145928
[33] Battilani, P., Giorni, P., Bertuzzi, T., Formenti, S. and Pietri, A. (2006) Black aspergilli and ochratoxin A in grapes in Italy. International Journal of Food Microbiology, 111, S53-S60.
http://dx.doi.org/10.1016/j.ijfoodmicro.2006.03.006
[34] Sage, L., Krivobok, S., Delbos, E., Seigle-Murandi, F. and Creppy, E.E. (2002) Fungal flora and ochratoxin A production in grapes and musts from France. Journal of Agricultural and Food Chemistry, 50, 1306-1311.
http://dx.doi.org/10.1021/jf011015z

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