Isolation and Spasmolytic Evaluation of New Alkaloids from Dichrostachys cinerea (L.) Wight et Arn. (Fabaceae)

DOI: 10.4236/pp.2013.49095   PDF   HTML     3,664 Downloads   5,222 Views   Citations

Abstract

Dichrostachys cinerea (L.) Wight et Arn. (Fabaceae) root bark is used in Ivorian Traditional Medicine to treat asthma, which is a respiratory disorder characterized by inflammation and the restriction of tracheal muscles obstructing the air circulation. The tracheal relaxant effect of a crude aqueous-alcoholic extract of the plant root bark was previously shown. For the present study, alkaloids were isolated from the same extract and investigated ex vivo in C57Bl/6j mice isolated trachea contracted with carbachol 1 μM, in comparison with a reference bronchodilatator, i.e. salbutamol. Two extraction procedures allowed isolating 2 Alkaloids that monodimensional and bi-dimensional nuclear magnetic resonance (NMR) and mass specters allowed identifying a pyrolidine structure type nucleus with a long bi-hydroxyled alkyl chain. Alkaloid 1, carrier of a sugar, is a glycoside of Alkaloid 2. Both alkaloids induced similar spasmolytic effects, but Alkaloid 1 was more effective than Alkaloid 2 at 9 × 10-6 M (p 0.01), 3 × 10-5 M, and 9 × 10-5 M (p 0.001). Salbutamol induced its spasmolytic effect in a different way, and its maximal effect Emax (less than 30%) was obtained at 9 × 10-6 M, while Emax of both alkaloids (100%) was obtained at 3 × 10-4 M.

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A. N’guessan-Irié, J. Dade, P. Champy, N. Siransy-Kouakou and V. Leblais, "Isolation and Spasmolytic Evaluation of New Alkaloids from Dichrostachys cinerea (L.) Wight et Arn. (Fabaceae)," Pharmacology & Pharmacy, Vol. 4 No. 9, 2013, pp. 684-688. doi: 10.4236/pp.2013.49095.

Conflicts of Interest

The authors declare no conflicts of interest.

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