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Genetic Relatedness of Diplostomum Species (Digenea: Diplostomidae) Infesting Nile Tilapia (Oreochromis Niloticus L.) in Western Kenya

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DOI: 10.4236/ojapps.2013.38055    3,522 Downloads   5,784 Views   Citations


Diplostomum species metacercariae are trematode parasites that pose serious economic threats to aquaculture practice globally. Identification of Diplostomum at metacercariae stage has remained equivocal, hence lack of elucidation of the actual role of these species in fish population. 21 Diplostomoid metacercariae obtained from eyes of Nile tilapia were characterized using 18S and ITS rDNA (ITS1-5.8S-ITS2) genes. Phylogenetic analyses of ITS rDNA gene dataset in the metacercariae revealed close relationship to Diplostomum mashonense and D. baeri. Molecular identification using 18S rDNA sequences revealed close relationship to D. compactum, D. phoxini and D. spathaceum. Overall, genetic analyses in this study depicted a significant unrecognized genetic diversity among Diplostomum species. Successful differentiation of Diplostomum genera in this study using ribosomal markers suggested that 18S and ITS rDNA genes are effective genetic markers for inter-species phylogenetic analysis and should be employed in future for identification of diplostomoidea.

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Cite this paper

V. Ndeda, D. Owiti, B. Aketch and D. Onyango, "Genetic Relatedness of Diplostomum Species (Digenea: Diplostomidae) Infesting Nile Tilapia (Oreochromis Niloticus L.) in Western Kenya," Open Journal of Applied Sciences, Vol. 3 No. 8, 2013, pp. 441-448. doi: 10.4236/ojapps.2013.38055.


[1] L. H. Chappell, L. J. Hardie and C. J. Secombes, “Diplostomiasis: The Disease and Host Parasite Interactions,” In: A. W. Pike and J. W. Lewis, Eds., Parasitic Diseases of Fish, Samara Publishing Limited, Otley, 1994, pp. 59-86.
[2] L. H. Chappell, “The Biology of Diplostomatid Eye Flukes of Fishes,” Journal of Helminthology, Vol. 69, No. 2, 1995, pp. 97-101.
[3] J. N. Stables and L. H. Chappell, “The Epidemiology of Diplostomiasis in Farmed Rainbow Trout from North-East Scotland,” Parasitology, Vol. 92, No. 3, 1986, pp. 699-710.
[4] J. S. Field and S. W. Irwin, “The Epidemiology, Treatment and Control of Diplostomiasis on a Fish Farm in Northern Ireland,” In: A. W. Pike and J. W. Lewis, Eds., Parasitic Diseases of Fish, Samara Publishing Limited, Otley, 1994, pp. 87-100.
[5] D. J. Marcogliese, P. Dumont, A. D. Gendron, Y. Mailhot, E. Bergeron and J. D. McLaughlin, “Spatial and Temporal Variation in Abundance of Diplostomum spp. in Wall-eye (Stizostedion vitreum) and White Suckers (Catostomus commersoni) from the St. Lawrence River,” Canadian Journal of Zooolgy, Vol. 79, 2001, pp. 355-369.
[6] C. R. Sangster, A. D. M. Dove and P. R. Bowser, “Diplostomum in Pond-Reared Walleye Stizostedion vitreum—Implications of a Management Strategy for Control,” Aquaculture, Vol. 236, No. 1-4, 2004, pp. 95-102.
[7] K. Buchmann and A. Uldal, “Effects of Eyefluke Infections on Growth of Rainbow Trout (Oncorhynchusmykiss) in Amariculture System,” Bulletin of European Association of Fish Pathology, Vol. 14, 1994, pp. 104-107.
[8] T. Paaver, R. Gross and P. Ilves, “Growth Rate, Maturation Level and Flesh Quality of Three Strains of Large Rainbow Trout (Oncorhynchus mykiss) Reared in Estonia,” Aquaculture International, Vol. 12, No. 1, 2004, pp. 33-45.
[9] A. Voutilainen, K. Figueiredo and H. Huuskonen, “Effects of the Eye Fluke Diplostomum spathaceum on the Energetics and Feeding of Arctic Charr Salvelinus Alpines,” Journal of Fish Biology, Vol. 73, No. 9, 2008, pp. 2228-2237.
[10] O. Seppälä, A. Karvonen and E. T. Valtonen, “Manipulation of Fish Host by Eye Flukes in Relation to Cataract Formation and Parasite Infectivity,” Animal Behaviour, Vol. 70, No. 4, 2005, pp. 889-894.
[11] M. Beverley-Burton, “A New Strigeid, Diplostomum (Tylodelphys) mashonense n. sp., (Trematoda: Diplostomatidae), from the Grey Heron, Ardea cinerea L. in Southern Rhodesia, with an Experimental Demonstration of Part of the Life Cycle,” Revue de Zoologie et de Botanique Africaines, Vol. 68, 1963, pp. 291-308.
[12] P. H. King and J. G. Van As, “Description of the Adult and Larval Stages of Tylodelphys xenopi (Trematoda: Diplostomidae) from Southern Africa,” Journal of Parasitology, Vol. 83, No. 2, 1997, pp. 287-295.
[13] A. Kostadinova, “A Checklist of Macroparasites of Liza Haematocheila (Temminck & Schlegel 1845) (Mugilidae),” Parasites and Vectors, Vol. 1, 2008, p. 48.
[14] C. J. Mwita and G. Nkwengulila, “Determinants of the Parasite Community of Clariid Fishes from Lake Victoria, Tanzania,” Journal of Helminthology, Vol. 82, No. 1, 2008, pp. 7-16.
[15] C. J. Mwita and G. Nkwengulila, “Phylogenetic Relationships of the Metazoan Parasites of the Clariid Fishes of Lake Victoria Inferred from Partial 18S rDNA Sequences,” Tanzanian Journal of Sciences, Vol. 37, 2011, pp. 179-185.
[16] F. D. Chibwana and G. Nkwengulila, “Variation in the Morphometrics of Diplostomid Metacercariae (Digenea: Trematoda) Infecting the Catfish, Clarias gariepinus in Tanzania,” Journal of Helminthology, Vol. 84, No. 1, 2010, pp. 61-70.
[17] F. Chibwana, I. Blasco-Costa, S. Georgieva, M. K Hosea, G. Nkwengulila, T. Tomaš Scholz and A. Kostadinov, “First Insight into the Barcodes for African Diplostomids (Digenea: Diplostomidae): Brain Parasites in Clarias gariepinus (Siluriformes: Clariidae),” Infection Genetics and Evolution, Vol. 17, 2013, pp. 62-70.
[18] G. K. Matolla, “Parasites of Tilapia (Oreochromis niloticus) in Chepkoilel-Fish Farm of Moi University, Eldoret, Kenya,” In: S. S. Drexler and H. Waidbacher, Eds., Proceedings of the 6th Framework Programme on BOMOSA Projects: Integrating BOMOSA Cage Fish Farming System in Reservoirs, Ponds and Temporary Water Bodies in Eastern Africa, Machakos, 2-4 September 2009, p. 28.
[19] E. M. Kembenya, G. K. Matolla, V. Ochieng’ and M. Ngaira, “Diplostomum Parasites Affecting Oreochromis niloticus in Chepkoilel Fish Farm and Two Dams in Eldoret-Kenya,” International Journal of Scientific & Engineering Research, Vol. 7, No. 3, 2012, pp. 1-7.
[20] G. N. Madanire-Moyo, W. J. Luus-Powell and P. A. S. Olivier, “Ecology of Metazoan Parasites of Clarias gariepinus (Osteichthyes: Clariidae) from the Nwanedi-Luphephe Dams of the Limpopo River System, South Africa,” African Zoology, Vol. 45, No. 2, 2010, pp. 233-243.
[21] J. April, R. L. Mayden, R. H. Hanner and L. Bernatcheza, “Genetic Calibration of Species Diversity among North America’s Freshwater Fishes,” PNAS, Vol. 108, No. 26, 2011, pp. 10602-10607.
[22] D. Gallazzo, E. S. Dayanandan, D. J. Marcogliese and J. D. McLaughlin, “Molecular Systematics of Some North American Species of Diplostomum (Digenea) Based on rDNA-Sequence Data and Comparisons with European Congeners,” Canadian Journal of Zoology, Vol. 30, 2002, pp. 2207-2217.
[23] S. Locke, J. D. Mclaughlin, S. Dayanandan and D. J. Marcogliese, “Diversity and Specificity in Diplostomum spp. Metacercariae in Freshwater Fishes Revealed by Cytochrome c Oxidase I and Internal Transcribed Spacer Sequences,” International Journal for Parasitology, Vol. 40, No. 3, 2010, pp. 333-343.
[24] S. Locke, J. D. Mclaughlin and D. J. Marcogliese, “DNA Barcodes Show Cryptic Diversity and a Potential Physiological Basis for Host Specificity among Diplostomoidea (Platyhelminthes: Digenea) Parasitizing Freshwater Fishes in the St. Lawrence River, Canada,” Molecular Ecology, Vol. 19, No. 13, 2010, pp. 2813-2827.
[25] K. Niewiadomska and Z. Laskowski, “Systematic Relationships among Six Species of Diplostomum nordmann, 1832 (Digenea) Based on Morphological and Molecular Data,” Acta Parasitologica, Vol. 47, No. 1, 2002, pp. 20-28.
[26] C. Rellstab, K. Louhi, A. Karvonen and J. Jokela, “Analysis of Trematode Parasite Communities in Fish Eye Lenses by Pyrosequencing of Naturally Pooled DNA,” Infection, Genetics and Evolution, Vol. 11, No. 6, 2011, pp. 1276-1286.
[27] Republic of Kenya, “Nyando District Development Plan, Nairobi; Kenya,” 2002.
[28] National Environmental Management Authority [NEMA], “Strategy for Flood Management in Lake Victoria Basin, Kenya,” 2004.
[29] Lake Basin Development Authority, “Feasibility Study on Kano Plain Irrigation Project,” Vol. 1, No. 71, 1992.
[30] W. W. Daniel, “Biostatistics: A Foundation for Analysis in the Health Sciences,” John Wiley & Sons, New York, 1999.
[31] S. Yamaguti, “Synopsis of Digenetic Trematodes of Vertebrates,” Keigaku Publishing, Tokyo, 1971.
[32] G. E. Truett, R. L. Heeger, A. A. Mynatt, J. A. Truett and M. L. Warman, “Preparation of PCR-Quality Mouse Genomic DNA with Hot Sodium Hydroxide and Tris (HotSHOT),” BioTechniques, Vol. 29, 2000, pp. 52-54.
[33] A. Moszczynska, S. A. Locke, J. D. McLaughlin, D. J. Marcogliese and T. J. Crease, “Development of Primers for the Mitochondrial Cytochrome c Oxidase I Gene in Digenetic Trematodes Illustrates the Challenge of Barcoding Parasitic Helminthes,” Molecular Ecology Resources, Vol. 9, No. S1, 2009, pp. 75-82.
[34] K. Luton, D. Walker and D. Blair, “Comparison of Ribosomal Internal Transcribed Spacers from Two Congeneric Species of Flukes (Platyhelminthes: Trematoda: Digenea),” Molecular Biochemistry of Parasitology, Vol. 56, No. 2, 1992, pp. 323-328.
[35] K. Tamura, J. Dudley, M. Nei and S. Kumar, “MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0,” Molecular Biology and Evolution, Vol. 24, No. 8, 2007, pp. 1596-1599.
[36] M. S. Blouin, C. A. Yowell, C. H. Courtney and J. B. Dame, “Host Movement and the Genetic Structure of Populations of Parasitic Nematodes,” Genetics, Vol. 141, No. 3, 1995, pp. 1007-1014.
[37] F. Prugnolle, A. Theron, J. P. Pointier, R. Jabbour-Zahab, P. Jarne, P. Durand and T. de Meeus, “Dispersal in a Parasitic Worm and Its Two Hosts: Consequence for Local Adaptation,” Evolution, Vol. 59, No. 2, 2005, pp. 296-303.

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