Terahertz Time-Domain Spectroscopy to Identify and Evaluate Anomer in Lactose

DOI: 10.4236/ajac.2013.412092   PDF   HTML     3,692 Downloads   5,587 Views   Citations


Lactose powder consisting of α-D-lactose monohydrate and anhydrous β-D-lactose was nondestructively and quantitatively evaluated by transmission-type Terahertz time-domain spectroscopy (THz-TDS). An absorption with peak at 39.7 cm-1 (1.19 THz) was assigned to be derived from anhydrous β-D-lactose, in addition to the absorptions due to α-D-lactose monohydrate with peak at 17.1 cm-1 (0.53 THz) and 45.6 cm-1 (1.37 THz). After deconvolution of the spectra using Lorentzian, integrated intensities of the absorptions with peak at 39.7 cm-1 and 45.6 cm-1 were uniquely dependent on the weight composition ratio of the α-and β-lactose powder. As a result, the net molar-ratio of the α-and β-lactose in lactose powder could be precisely evaluated by the integrated intensity ratio. Further, anomer content in lactose powder extracted from lactose solution was evaluated and the refined and unrefined features were shown by the evaluation method.

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S. Yamauchi, S. Hatakeyam, Y. Imai and M. Tonouchi, "Terahertz Time-Domain Spectroscopy to Identify and Evaluate Anomer in Lactose," American Journal of Analytical Chemistry, Vol. 4 No. 12, 2013, pp. 756-762. doi: 10.4236/ajac.2013.412092.

Conflicts of Interest

The authors declare no conflicts of interest.


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