Ruminal degradability of Guinea grass silage inoculated with Streptoccocus bovis isolated from bovine rumen combined or not with com wheat bran

Abstract

This experiment aimed to evaluate the ruminal degradability in situ of the dry matter (DM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) of Guinea grass silages. The experimental design was completely randomized with six treatments and six repetitions: Guinea grass silage, Guinea grass silage inoculated with 10% wheat bran, Guinea grass silage inoculated with 106 cfu/g of strains of Streptococcus bovis JB1; Guinea grass silage inoculated with 106 cfu/g of strains of Streptococcus bovis JB1 plus 10% of wheat bran; Guinea grass silage inoculated with 106 cfu/g of strains of Streptococcus bovis HC5; Guinea grass silage inoculated with 106 cfu/g of strains of Streptococcus bovis HC5 plus 10% of wheat bran. The silages inoculated with Streptococcus bovis and with added wheat bran resulted in higher values of ruminal degradability of DM, 32.76% and 32.17%, and of crude protein 38.28% and 37.89%. For the effective and potential degradability of DM, CP, NDF and ADF the highest values occurred for silages in these silages as well, in all passage rates. There is a similarity between the responses of Streptococcus bovis JB1 and the HC5, and the wheat bran enhanced the effectiveness of the microbial additive.

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Bonelli, E. , Zanine, A. , Souza, A. , Ferreira, D. and Alves, G. (2013) Ruminal degradability of Guinea grass silage inoculated with Streptoccocus bovis isolated from bovine rumen combined or not with com wheat bran. Agricultural Sciences, 4, 628-634. doi: 10.4236/as.2013.412084.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Mcdonald, P., Henderson, A.R. and Heron, S.J. (1991) The biochemistry of silage. 2nd Edition, Chalcombe Publications, Marlow.
[2] Coan, R.M., Reis, R.A., Garcia, G.R., Schockenl Turrino, R.P., Ferreira, D.S., Resende, F.D. and Gurgel, F.A. (2007) Microbiological and fermentative dynamics of Guinea grass and marandu grass silage using pelleted citrus pulp as an additive. Brazilian Journal of Animal Science, 36, 1502-1511.
[3] Zanine, A.M., Lana, R.P., Santos, E.M., Ferreira, D.J. and Pereira, O.G. (2007) Microbial population and nutritional compounds in the organs of Guinea grass before and after ensilage. Agricultural Sciences, 28, 143-150.
[4] Coelho, R.M. (2002). Effects of the dry matter concentration and use of bacterial-enzymatic inoculant, in tifton 85 (Cynodon spp.) silage, on the nutrient digestion, ruminal parameters and ingestive behavior in growing beef steers. Ph.D. Thesis, School of Agriculture Luiz de Queiroz, Piracicaba.
[5] Ferreira, D.J. (2010) Streptococcus bovis isolated from the rúmen as an additive for silage, elephant grass. Ph.D. Thesis, Federal University of Viçosa, Viçosa.
[6] Penteado, D.C.S., Santos, E.M., Carvalho, G.G.P., Oliveira, J.S., Zanine, A.M., Pereira, O.G. and Ferreira, D.J.F. (2007) Lactobacilos plantarum from microbiota as inoculant for Panicum maximum silage. Archives of Animal Science, 56, 191-202.
[7] Chunjian, L., Bolsen, B. E. and Fung, D. Y. C. (1992) Epiphytic lactic acid bacteria succession during the preensiling periods of alfafa and maize. Jornal Applied Bacteriology, 46, 375-387.
[8] Jones, B.A., Muck, R.E. and Rick, S.C. (1991) Selection and application of Streptococcus bovis as a silage inoculant. Journal Applied Bacteriology, 57, 3000-3005.
[9] Oliveira, J.S., Santos, E.M., Zanine, A.M., Mantovani, H.C., Lílian, O.R. and Pereira, O.G. (2007) Microbial populations and chemical composition of Panicum maximum grass silage inoculated with Streptococcus bovis isolated from the rumen. Archivos Veterinay Science, 12, 35-40.
[10] Zanine, A.M., Lana, R.P., Santos, E.M., Ferreira, D.J., Pereira, O.G., Mantovani, H.C. and Dorea, J.R.R. (2008) Effect of inoculation with Enterococcus faecium and Streptococcus bovis isolated from bovine rumen on fermentation in silage of elephant grass. 45th Annual Meeting of the Brazilian Society of Animal Science, Lavras, June 2008, 1-3.
[11] Ferreira, D.J., Zanine, A.M., Santos, E.M., Lana, R.P., Silva, W.L., Souza, A.L. and Pereira, O.G. (2011). Fermentation and nutritive value of elephant grass silage inoculated with streptococcus bovis. Archives of Animal Science, 60, 110-124.
[12] Ferreira, D.J., Lana, R.P., Zanine, A.M., Santos, E.M. and Veloso, C.M. (2013) Silage fermentation and chemical composition of elephant grass inoculated with rumen strains of Streptococcus bovis. Animal Feed Science and Technology, 183, 22-28.
http://dx.doi.org/10.1016/j.anifeedsci.2013.04.020
[13] Santos, E.M. (2007) Microbial populations and fermentation profile in tropical grass silages and performance of beef cattle fed diets containing mombaça grass silages. Ph.D. Thesis, Federal University of Viçosa, Viçosa.
[14] Bolsen, K.K., Lin, C., Brent, C.R., Feyerherm, A.M., Urban, J.E. and Aimutis W.R. (1992) Effect the silage additives on the microbial succession and fermentation process of alfalfa and corn silages. Journal Dairy Science, 75, 3066-3083.
http://dx.doi.org/10.3168/jds.S0022-0302(92)78070-9
[15] Kung Jr., L. and Ranjit, N.K. (2001) The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. Journal Dairy Science, 84, 1149-1155.
http://dx.doi.org/10.3168/jds.S0022-0302(01)74575-4
[16] Silva, D.J. and Queiroz A.C. (2002) Food analysis: Chemical and biological methods. UFV, Viçosa.
[17] Nocek, J.E. (1988) In situ and other methods to estimate ruminal protein and energy digestibility. A review. Journal of Dairy Science, 71, 2051-2069.
http://dx.doi.org/10.3168/jds.S0022-0302(88)79781-7
[18] National Research Council (NRC) (2001) Nutrient requeriments of dairy cattle. National Research Council, Washinton DC.
[19] Orskov, E.R. and Mcdonald, I. (1979) The estimation of protein degradability in the rumen from incubation weighted according to rate of passage. Journal Agricutural Science, 92, 499-503.
http://dx.doi.org/10.1017/S0021859600063048
[20] Waldo, D.R., Smith, L.W. and Cox, E.L. (1972) Model of cellulose disappearance from the rumen. Journal Dairy Science, 55, 125-129.
http://dx.doi.org/10.3168/jds.S0022-0302(72)85442-0
[21] Statistical Analysis System (SAS) (2002) User’s guide. SAS Institute, Cary.
[22] Knicky, M. (2005) Possibilities to improve silage conservation. Doctoral Thesis, Swedish University of Agricultural Sciences, Uppsala.
[23] Henriques, L.T., Coelho, S.J.F., Vasquez, H.M., Araújo, G.G.L., Cecon, P.R., Detmann, E. and Barros, E.E.L. (2004) Effect of acipin on the degradability and rate of passage of elephant-grass and corn silages in Holstein × Zebu cattle. Brazilian Archives of Veterinary Medicine and Animal Science, 56, 757-763.
[24] Pires, A.J.V., Reis, R.A., Carvalho, G.G.P., Siqueira, G.R., Bernardes, T.F., Ruggieri, A.C. and Roth, M.T.P. (2010). Ruminal degradability of dry matter, crud protein, and fiber fraction of silages of corn, sorghum, and Brachiaria brizantha. Brazilian Archives of Veterinary Medicine and Animal Science, 62, 391-400.
[25] Tonani, F.L., Ruggieri, A.C., Queiroz, A.C. and Andrade, P. (2001) Ruminal in situ degradability of dry matter and neutral detergent fiber of sorghum (Sorghum bicolor L.) silages with different harversting times. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 53, 200-2013.
[26] National Research Council (NRC) (1985) Ruminant nitrogen usage. National Academy Press, Washington DC.
[27] Chesson, A., Gordon, A.H. and Lomax, J.A. (1985) Methylation analysis of mesophyll, epidermis and fibre cells —Walls isolated from the leaves of perennial and Italian rygrass. Carbohydrate Research, 28, 137-147.
http://dx.doi.org/10.1016/S0008-6215(00)90762-6
[28] Muck, R. (1996) Inoculation of silage and its effects on silage quality. Proceedings of the Informational Conference on Dairy and Forage Industry, Dairy Research Centre, Madison, September 1996, 43-51.
[29] Cabral, L.S., Valadares Filho, S.C. and Zervoudakis, J.T. (2005). In situ degradability of dry matter, crude protein and fiber of some feeds. Brazilian Agricultural Research, 40, 777-781.
[30] Mertens, D.R. (1996) Using fiber and carbohydrate analyses to formulate dairy rations. Informational Conference with Dairy and Forage Industries, Wisconsin, April 1996, 81-92.
[31] Van Soest, P.J. (1994) Nutritional ecology of the ruminant. 2nd Edition, Cornell University Press, Ithaca.

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