Germicidal Action of Some Metals/Metal Ions in Combating E. coli Bacteria in Relation to Their Electro-Chemical Properties

DOI: 10.4236/jwarp.2013.512119   PDF   HTML     4,005 Downloads   6,322 Views   Citations


The germicidal properties of some metals and metal compounds were investigated in relation to their electro-chemical properties that may play a role in the inactivation of E. coli bacteria. These properties included the atomic and ionic radii, ionization energy, oxidation state, energy of formation with hydro-sulfide groups, and the redox potential of the metals. Cultures of E. coli bacteria with predetermined numbers of colony-forming units (CFU’s) were brought in contact with the metals as well as metal compounds, using Eosin methylene blue agar medium and sterilized, distilled water. The rate of inactivation was determined by counting the CFU’s at predefined intervals of time after inoculation. The experimental results showed that the rate of inactivation increases with increasing ionization energy of the metals. While the rate of inactivation increases with decreasing atomic radii for some of the transition metals, there is no apparent relationship between ionic radius and rate of inactivation for the metal compounds. In addition, non-transition group III metals such as aluminum and indium showed higher rates of inactivation that are comparable to the action of silver. This is probably due to the increase in coagulation potential and the resulting adsorption of bacteria, because a larger number of ions are able electrons carried by these atoms. In general, there is a difference between the atoms and the ions in terms of their rate of inactivation. This difference increases amongst the transition metals that have lower oxidation potential, lower ionization potential as well as larger ionic radius. The results also showed that for the metals, adsorption through coagulation is an important fact or that is responsible for inactivation of E. coli. For the metal compounds, additional mechanisms such as direct reaction through complex formation, physico-chemical distortion of the cell structure through direct entry of the ions into the cell, may contribute towards greater inactivation.

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A. Varkey, M. Dlamini, A. Mansuetus and A. Tiruneh, "Germicidal Action of Some Metals/Metal Ions in Combating E. coli Bacteria in Relation to Their Electro-Chemical Properties," Journal of Water Resource and Protection, Vol. 5 No. 12, 2013, pp. 1132-1143. doi: 10.4236/jwarp.2013.512119.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] R. C. Weast, “CRC Handbook of Chemistry and Physics,” 64th Edition, CRC Press, Boca Raton, 1984.
[2] D. H. Nies, “Microbial Heavy-Metal Resistance,” Applied Microbiology and Biotechnology, Vol. 51, No. 6, 1999, pp. 730-750.
[3] M. Valls and V. de Lorenzo, “Exploiting the Genetic and Biochemical Capacities of Bacteria for the Remediation of Heavy Metal Pollution,” FEMS Microbiological Reviews, Vol. 26, No. 4, 2002, pp. 327-338.
[4] D. L. Godbold and A. Hüttermann, “Effect of Zinc, Cadmium and Mercury on Root Elongation of Piceaabies (Karst.) Seedlings, and the Significance of These Metals to Forest Die-Back,” Environmental Pollution, Vol. 38, No. 4, 1985, pp. 375-381.
[5] C. W. Breckle, “Growth under Heavy Metals,” In: Y. Waisel, A. Eshel and U. Kafkafi, Eds., Plant Roots: The Hidden Half, Marcel Dekker, New York, 1991, pp. 351-373.
[6] T. J. Berger, J. A. Sparado, S. E. Chapin and R. O. Becker, “Electrically Generated Silver Ions: Quantitative Effects on Bacterial and Mammalian Cells,” Anti Microb. Agents, 1996, pp. 357-358.
[7] J. J. Hostynek and H. I. Maibach, “Hypersensitivity: Dermatologic Aspects—An Overview,” Reviews on Environmental Health, Vol. 153, No. 3, 2003, pp. 153-183.
[8] G. Borkow and J. Gabbay, “Copper as a Biocidal Tool,” Current Medicinal Chemistry, Vol. 12, No. 18, 2005, pp. 2163-2175.
[9] M. Valko, H. Morris and M. T. Cronin, “Metals, Toxicity and Oxidative Stress,” Current Medicinal Chemistry, Vol. 12, No. 10, 2005, pp. 1161-1208.
[10] H. Rieth, “Killing of Pathogenic Fungi with Organic Zinc Compounds. Fungicidal Aerosol Disinfection and Accumulative Impregnation,” Mykosen, Vol. 11, No. 9, 1968, pp. 667-670.
[11] I. K. Konstantinou and A. T Albanis, “Worldwide Occurrence and Effects of Antifouling Paint Booster Biocides in the Aquatic Environment: A Review,” Environment International, Vol. 30, No. 2, 2004, pp. 235-248.
[12] R. Eisler, “Zinc Hazard to Fish, Wildlife, and Invertebrates: A Synoptic Review,” Contaminant Hazard Reviews, US Department of the Interior, Fish and Wildlife Service, Laurel, 1993.
[13] J. H. Kim, H. Cho, S. E. Ryu and M. U. Choi, “Effects of Metal Ions on the Activity of Protein Tyrosine Phosphatase VHR: Highly Potent and Reversible Oxidative Inactivation by Cu2+ Ion,” Archives of Biochemistry and Biophysics, Vol. 382, No. 1, 2000, pp. 72-80.
[14] A. R. Karlstrom and R. L. Levine, “Copper Inhibits the Protease from Human Immunodeficiency Virus 1 by Both Cysteine-Dependent and Cysteine-Independent Mechanisms,” Proceedings of the National Academy of Sciences, Vol. 88, No. 13, 1991, pp. 5552-5556.
[15] C. Cervantes and F. Gutierrez-Corona, “Copper Resistance Mechanisms in Bacteria and Fungi,” FEMS Microbiology Reviews, Vol. 14, No. 2, 1994, pp. 121-137.
[16] Y. Ohsumi, K. Kitamoto and Y. Anraku, “Changes Induced in the Permeability Barrier of the Yeast Plasma Membrane by Cupric Ion,” Journal of Bacteriology, Vol. 170, No. 6, 1988, pp. 2676-2682.
[17] S. K. Stosh and D. Bagchi, “Oxidative Mechanisms in the Toxicity of Metal Ions,” Free Radical Biology & Medicine, Vol. 18, No. 2, 1995, pp. 321-336.
[18] P. R. Blackett, D. M. Lee, D. L. Donaldson, J. D. Fesmire, W. Y. Chan, J. H. Holcombe and O. M. Rennert, “Studies of Lipids, Lipoproteins, and Apolipoproteins in Menkes’ Disease,” Pediatric Research, Vol. 18, No. 9, 1984, pp. 864-870.
[19] A. H. Ding and P. C. Chan, “Singlet Oxygen in Copper-Catalyzed Lipid Peroxidation in Erythrocyte Membranes,” Lipids, Vol. 19, No. 4, 1984, pp. 278-284.
[20] P. C. Chan, O. G. Peller and L. Kesner, “Copper (II)-Catalyzed Lipid Peroxidation in Liposomes and Erythrocyte Membrane,” Lipids, Vol. 17, No. 5, 1982, pp. 331-337.
[21] J. R. Hazel and E. E. Williams, “The Role of Alterations in Membrane Lipid Composition in Enabling Physiological Adaptation of Organisms to Their Physical Environment,” Progress in Lipid Research, Vol. 29, No. 3, 1990, pp. 167-227.
[22] S. V. Avery, J. L. Harwood and D. Lloyd, “Quantification and Characterization of Phagocytosis in the Soil Amoeba Acanthamoebacastellanii by Flow Cytometry,” Applied and Environmental Microbiology, Vol. 61, No. 3, 1995, pp. 1124-1132.
[23] H. Elzanowska, R. G. Wolcott, D. M. Hannum and J. K. Hurst, “Bactericidal Properties of Hydrogen Peroxide and Copper or Iron-Containing Complex Ions in Relation to Leukocyte Function,” Free Radical Biology & Medicine, Vol. 18, No. 3, 1995, pp. 437-449.
[24] A. V. Kachur, C. J. Koch and J. E. Biaglow, “Mechanism of Copper-Catalyzed Oxidation of Glutathione,” Free Radical Research, Vol. 28, No. 3, 1998, pp. 259-269.
[25] D. H. Nies and N. Brown, “Two-Component Systems in the Regulation of Heavy Metal Resistance,” In: S. Silver and W. Walden, Eds., Metal Ions in Gene Regulation, Chapman and Hall, London and New York, 1998, pp. 77-103.
[26] P. Gong, O. Y. Ogra and S. Koizumi, “Inhibitory Effects of Heavy Metals on Transcription Factor Sp1,” Industrial Health, Vol. 38, No. 2, 2000, pp. 224-227.
[27] C. Cervantes, J. Campos-Garcia, S. Devars, F. Gutierrez-Corona, H. Loza-Tavera, J. C. Torres-Guzman and R. Moreno-Sanchez, “Interactions of Chromium with Microorganisms and Plants,” FEMS Microbiology Reviews, Vol. 25, No. 3, 2001, pp. 335-347.
[28] J. D. L. Singh, Carlisle, D. E. Pritchard and S. R. Patierno, “Chromium-Induced Genotoxicity and Apoptosis: Relationship to Chromium Carcinogenesis,” Oncology Reports, Vol. 5, No. 6, 1998, 1307-1318.
[29] Y. Suzuki, T. Sato, H. Isobe, T. Kogure and T. Murakami, “Dehydration Processes of the Meta-Autunite Group Minerals, Meta-Autunite, Metasaléeite and Metatorbernite,” American Mineralogist, Vol. 70, No. 8-9, 2005, pp. 1308-1314.
[30] L. Nan, Y. Liu, M. Lu and K. Yang, “Study on Antibacterial Mechanism of Copper-Bearing Austenitic Antibacterial Stainless Steel by Atomic Force Microscopy,” Journal of Materials Science: Materials in Medicine, Vol. 19, No. 9, 2008, pp. 3057-3062.
[31] S. V. Avery, N. G. Howlett and S. Radice, “Copper Toxicity towards Saccharomyces Cerevisiae: Dependence on Plasma Membrane Fatty Acid Composition,” Applied and Environmental Microbiology, Vol. 62, No. 11, 1996, pp. 3960-3966.
[32] M. Mergeay, D. Nies, H. G. Schlrgrl, J. Gerits and P. Charles, “Alcaligeneseutorophus CH34 Is a Facultative Chemolithotroph with Plasmid-Bound Resistance to Heavy Metals,” Journal of Bacteriology, Vol. 162, No. 1, 1985, pp. 328-334.
[33] Q. K. Feng, J. Wu, G. Q. Chen, F. Z. Cui, T. N. Kim and J. O. Kim, “A Mechanistic Study of the Antibacterial Effect of Silver Ions on Escherichia coli and Staphylococcus Aureus,” Journal of Biomedical Materials Research, Vol. 52, No. 4, 2000, pp. 662-668.
[34] C. Rensing, B. Mitra and B. P. Rosen, “Insertional Inactivation of dsbA Produces Sensitivity to Cadmium and Zinc in Escherichia coli,” Journal of Bacteriology, Vol. 179, No. 8, 1997, pp. 2769-2771.
[35] A. L. Lehninger, D. L. Nelson and M. M. Cox, “Principles of Biochemistry,” 2nd Edition, New York, 1993.
[36] S. Y. Liau, D. C. Read, W. J. Pugh, J. R. Furr and A. D. Russell, “Interaction of Silver-Nitrate with Readily Identifiable Groups: Relationship to the Anti-Bacterial Action of Silver Ions,” Letters in Applied Microbiology, Vol. 25, No. 4, 1997, pp. 279-283.
[37] F. Hussain, E. Sedlak and P. Wittung-Stafshede, “Role of Copper in Folding and Stability of Cupredoxin-Like Copper-Carrier Protein CopC,” Archives of Biochemistry and Biophysics, Vol. 467, No. 1, 2007, pp. 58-66.
[38] F. Hussain and P. Wittung-Stafshede, “Impact of Cofactor on Stability of Bacterial (CopZ) and Human (Atox1) Copper Chaperones,” Biochimica et Biophysica Acta, Vol. 1774, No. 10, 2007, pp. 1316-1322.
[39] A. R. Karlstrom, B. D. Shames and R. L. Levine, “Reactivity of Cysteine Residues in the Protease from Human Immunodeficiency Virus: Identification of a Surface-Exposed Region Which Affects Enzyme Function,” Archives of Biochemistry and Biophysics, Vol. 304, No. 1, 1993, pp. 163-169.
[40] M. J. Davies, B. C. Gilbert and R. M. Haywood, “Radical-Induced Damage to Proteins: E.S.R. Spin-Trapping Studies,” Free Radical Research, Vol. 15, No. 2, 1991, pp. 111-127.
[41] R. T. Dean, S. P. Wolff and M. A. McElligott, “Histidine and Proline are Important Sites of Free Radical Damage to Proteins,” Free Radical Research, Vol. 7, No. 2, 1989, pp. 97-103.
[42] R. B. Martin and Y. H. Mariam, “Metal Ions in Solution,” Marcel Dekker, Basel, 2001.
[43] K. L. Sagripanti, M. L. Swicord and C. C. Davis, “Microwave Effects on Plasmid DNA,” Radiation Research, Vol. 110, No. 2, 1987, pp. 219-231.
[44] B. H. Geierstanger, T. F. Kagawa, S. L. Chen, G. J. Quigley and P. S. Ho, “Basespecific Binding of Copper (II) to Z-DNA. The 1.3-A Single Crystal Structure of d(m5CGUAm5CG) in the Presence of CuCl2,” The Journal of Biological Chemistry, Vol. 266, No. 30, 1991, pp. 20185-20191.
[45] E. Keyhani, F. Abdi-Oskouei, F. Attar and J. Keyhani, “DNA Strand Breaks by Metal-Induced Oxygen Radicals in Purified Salmonella Typhimurium DNA,” Annals of the New York Academy of Sciences, Vol. 1091, No. 1, 2006, pp. 52-64.
[46] M. R. Gunther, P. M. Hanna, R. P. Mason and M. S. Cohen, “Hydroxyl Radical Formation from Cuprous Ion and Hydrogen Peroxide: A Spin-Trapping Study,” Archives of Biochemistry and Biophysics, Vol. 316, No. 1, 1995, pp. 515-522.
[47] L. Macomber, C. Rensing and J. A. Imlay, “Intracellular Copper does not Catalyze the Formation of Oxidative DNA Damage in Escherichia coli,” Journal of Bacteriology, Vol. 189, No. 5, 2007, pp. 1616-1626.
[48] A. Schrammel, D. Koesling, A. C. Gorren, M. Chevion, K. Schmidt and B. Mayer, “Inhibition of Purified Soluble Guanylyl Cyclase by Copper Ions,” Biochemical Pharmacology, Vol. 52, No. 7, 1996, pp. 1041-1045.
[49] R. Pedahzur, H. L. Shuval and S. Ulitzur, “Silver and Hydrogen Peroxide as Potential Drinking Water Disinfectants: Their Bactericidal Effects and Possible Modes of Action,” Water Science and Technology, Vol. 35, No. 11-12, 1997, pp. 87-93.
[50] J. Aiyar, H. J. Berkovits, R. A. Floyd and K. E. Wetterhahn, “Reaction of Chromium (VI) with Hydrogen Peroxide in the Presence of Glutathione: Reactive Intermediates and Resulting DNA Damage,” Chemical Research Toxicology, Vol. 3, No. 6, 1990, pp. 595-603.
[51] K. E. Wetterhahn, J. W. Hamilton, J. Aiyar, K. M. Borges and R. Floyd, “Mechanisms of Chromium(VI) Carcinogenesis. Reactive Intermediates and Effect on Gene Expression,” Biological Trace Element Research, Vol. 21, No. 1, 1989, pp. 405-411.
[52] M. A. S. Fernandez, M. S. Santos, M. C. Alpoim, V. M. C. Madeira and J. A. F. Vicente, “Chromium (VI) Interaction with Plant and Animal Mitochondrial Bioenergetics: A Comparative Study,” Journal of Biochemical and Molecular Toxicology, Vol. 16, No. 2, 2002, pp. 53-63.
[53] C. Barata, S. J. Markich, D. J. Baird, G. Taylor and A. M. V. M. Soares, “Genetic Variability in Sub Lethal Tolerance to Mixtures of Cadmium and Zinc in Clones of Daphnia magna Straus,” Aquatic Toxicology, Vol. 60, No. 1-2, 2002, pp. 85-99.
[54] K. Apel and H. Hirt, “Reactive Oxygen Species: Metabolism, Oxidative Stress, and Signal Transduction,” Annual Review of Plant Biology, Vol. 55, No. 1, 2004, pp. 373-399.
[55] P. Hu, E. L. Brodie, Y. Suzuki, H. H. McAdams and G. L. Andersen, “Whole-Genome Transcriptional Analysis of Heavy Metal Stresses in Caulobactercrescentus,” Journal of Bacteriology, Vol. 187, No. 24, 2005, pp. 8437-8449.
[56] C. Manzl, J. Enrich, H. Ebner, R. Dallinger and G. Krumschnabel, “Copper-Induced Formation of Reactive Oxygen Species Causes Cell Death and Disruption of Calcium Homeostasis in Trout Hepatocytes,” Toxicology, Vol. 196, No. 1-2, 2004, pp. 57-64.
[57] R. O. Wright and A. Baccarelli, “Metals and Neurotoxicology,” The Journal of Nutrition, Vol. 137, No. 12, 2007, pp. 2809-2813.
[58] K. K. Das, S. N. Das and S. A. Dhundasi, “Nickel, Its Adverse Health Effects & Oxidative Stress,” The Indian Journal of Medical Research, Vol. 128, No. 4, 2008, pp. 412-425.

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