Impact of a physical activity program on plasma concentrations of adiponectin, leptin and ghrelin in overweight and obese schoolchildren: A randomized controlled trial
André Everton de Freitas, Joel Alves Lamounier, Danusa Dias Soares, Thiago Henrique de Oliveira, Débora Romualdo Lacerda, Joyce Batista Andrade, Flávia Komatsuzaki, Leida Maria Botion, Adaliene Versiani Matos Ferreira, Camilo Adalto Mariano da Silva, Dilermando Fazito Rezende
Faculty of Medicine Barbacena, Barbacena, Brazil.
Graduate Program in Child and Teenager Health, Medicine School, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Graduate Program in Child and Teenager Health, Medicine School, Federal University of Minas Gerais, Belo Horizonte, Brazil Faculty of Health and Human Ecology, Medicine School, Vespasiano, Brazil;.
Graduate Program in Health Sciences, Medicine School, Federal University of S?o Jo?o Del Rei, Campus Midwest, Divinópolis, Brazil.
Laboratory of Exercise Physiology, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Laboratory of Metabolism, Federal University of Minas Gerais, Belo Horizonte, Brazil.
School of Nursing, Federal University of Minas Gerais, Belo Horizonte, Brazil.
School of Nutrition, Federal University of Ouro Preto, Ouro Preto, Brazil.
DOI: 10.4236/health.2013.511245   PDF    HTML     4,523 Downloads   6,528 Views   Citations


Introduction: Child obesity has important consequences on the child’s health and is related to the risk of developing chronic diseases. That brings up the need for research assessing the impact of obesity-and overweight-reduction programs with close attention to methodology aspects and precise indicators of the impact of such programs. Goal: Assessing the impact of a physical activity program on the plasma concentrations of adiponectin, leptin, and ghrelin in overweight and obese schoolchildren. Methods: Randomized controlled trial, double-blind, with 6-to 9-year-old overweight and obese school-children. A sample of 79 schoolchildren randomly distributed into control and intervention groups was subjected to a physical activity program. The levels of adiponectin, leptin, ghrelin, body mass index, body fat percentage, anthropometry, lipid profile and glycemia were evaluated. The program’s impact was assessed through multivariate linear regression models. Result: When comparing the groups, a significant difference was found for adiponectin, which shows an increase in the proportion of subjects with higher adiponectin concentrations for the intervention group and a reduction in body mass index and body fat percentage. Conclusion: The impact of the intervention program was positive in increasing adiponectin levels associated with a reduction in body mass index and body fat percentage.

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Freitas, A. , Lamounier, J. , Soares, D. , Oliveira, T. , Lacerda, D. , Andrade, J. , Komatsuzaki, F. , Botion, L. , Ferreira, A. , Silva, C. and Rezende, D. (2013) Impact of a physical activity program on plasma concentrations of adiponectin, leptin and ghrelin in overweight and obese schoolchildren: A randomized controlled trial. Health, 5, 1819-1828. doi: 10.4236/health.2013.511245.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Pénicaud, L., Cousin, B., Leloup, C., Lorsignol, A. and Casteilla, L. (2000) The autonomic nervous system, adipose tissue plasticity, and energy balance. Nutrition, 16, 903-908.
[2] Saleh, J., Sniderman, A.D. and Cianflone, K. (2000) Regulation of plasma fatty acid metabolism. Clinica Chimica Acta, 286, 163-180.
[3] Ahima, R.S. and Flier, J.S. (2000) Adipose tissue as an endocrine organ. Trends in Endocrinology and Metabolism, 11, 327-332.
[4] Arsian, N., Baris, E. and Aydin, A. (2010) Hormones and cytokines in childhood obesity. Indian Pediatrics, 47, 829-839.
[5] Ebbeling, C.B., Rawlak, D.B. and Ludwig, D.S. (2002) Childhood obesity: Public-health crisis, common sense cure. Lancet, 360, 473-482.
[6] Saelens, B.E., Grow, H.M., Stark, L.J., Seeley, R.J. and Roehrig, H. (2011) Efficacy of increasing physical activity to reduce children’s visceral fat: A pilot randomized controlled trial. International Journal of Obesity, 6, 102-112.
[7] Lloyd, L.J., Langley-Evans, S.C. and McMullen, S. (2012) Childhood obesity and risk of the adult metabolic syndrome: A systematic review. International Journal of Obesity, 36, 1-11.
[8] Ruchatand, S.M. and Mottola, M.F. (2012) Preventing long-term risk of obesity for two generations: Prenatal physical activity is part of the puzzle. Journal of Pregnancy, 2012, 1-33.
[9] Steen, S.N. (1994) Nutrition for young athletes: Special considerations, Sports Medicine, 17, 152-164.
[10] Summerbell, C.D., Waters, E., Edmunds, L.D., Kelly, S., Brown, T. and Campbell, K.J. (2005) Interventions for preventing obesity in children. The Cochrane Database of Sistematic Reviews, 20, 1-70.
[11] Garrow, J.S. and Webster, J. (1985) Quetelet’s index (W/ H2): As a measure of fatness. International Journal of Obesity, 9, 147-153.
[12] De Onis, M., Onyango, A.W., Borghi, E., Siyam, A., Nishida, C. and Siekmann, J. (2007) Development of a WHO growth reference for school-aged children and adolescents. Bulletin of the World Health Organization, 85, 660-667.
[13] Taylor, R.W., Jones, I.E., Williams, S.M. and Goulding, A. (2000) Evaluation of waist circumference, waist-to-hip ratio, and conicity index as screening tools for high trunk fat mass, as measures by dual-energy by dual-energy X-ray absorptiometry, in children aged 3-19 y. The American Journal of Clinical Nutrition, 72, 490-495.
[14] Heyward, V.H. and Stolarczyl, L.M. (2000) Avaliacao da composicao corporal. Manole, Sao Paulo, 243 p.
[15] Slaughter, M.H., Lohman, T.G., Boileau, R.A. et al. (1988) Influence of maturation on relationship of skinfold to body density: A cross-sectional study. Human Biology, 56, 681-689.
[16] Friedewald, W.T., Levy, R.I. and Fredrickson, D.S. (1972) Estimation of the concentration of low-density lipoprotein cholesterolin plasma, without use of the preparative ultracentrifuge. Clinical Chemistry, 18, 499-502.
[17] Ferguson, M.A., White, L.J., McCoy, S., Kim, H.W., Petty. T. and Wilsey, J. (2003) Plasma adiponectin response to acute exercise in health subjects. European Journal of Applied Physiology, 91, 324-329.
[18] Koistinen, H.A. Tominen, J.A. Ebeling, P., Heiman, M.L., Stephens, T.W. and Koivisto, V.A. (1998) The effect of exercise on leptin concentration in health men and type 1 diabetic patients. Medicine and Science in Sports and Exercise, 30, 805-810.
[19] Foster-Schubert, K. Mctiernan, A. Frayo, S., et al. (2005) Human plasma ghrelin levels increase during a one-year exercise program. The Journal of Clinical Endocrinolology and Metabolism, 90, 820-825.
[20] Verhagen, A., De Vet, H., De Bie, R., et al. (1998) The Delphi list: A criteria list for quality assessment of randomized clinical trials for conduction systematic reviews developed by Delphi consensus. Journal of Clinical Epidemiology, 51, 1235-1241.
[21] Friedmann J.M. and Halaas, J.L. (1998) Leptin and the regulation of body weight in mammals. Nature, 395, 763-770.
[22] Sandoval, D.A. and Davis, S.N. (2003) Leptin: Metabolic control and regulation. Journal of Diabetes and Its Complications, 17, 108-113.
[23] Considine, R.V., Sinha, M.K., Heiman, M.L., et al. (1996) Serum immunoreactiveleptin concentrations in normalweight and obese humans. The New England Journal of Medicine, 334, 292-295.
[24] Maffei, M.J., Haalas, J., Ravussin, E., et al. (1995) Leptin levels in humans and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Medicine, 11, 1155-1161.
[25] Leyva, F., Godsland, I.F., Ghatei, M., et al. (1998) Hyperleptinemia as a component of a metabolic syndrome of cardiovascular risk. Arteriosclerosis, Thrombosis and Vascular Biology, 18, 928-933.
[26] Vierhapper, H., Heinze, G., Nowotny, P. and Bieglmayer, C. (2003) Leptin and the control of obesity. Metabolism, 52, 379-381.
[27] Nakazato, M., Murakami, N., Date, Y., et al. (2001) A role for ghrelin in the central regulation of feeding. Nature, 409, 194-198.
[28] Ukkola O. and Poykoo, S. (2002) Ghrelin, growth and obesity. Annals of Medicine, 34, 102-108.
[29] Rosicka, M., Krsek, M., Matoulek, Z., et al. (2003) Serum ghrelin levels in obese patients: The relationship to serum leptin levels and soluble leptin receptors levels. Physiology Research, 52, 61-66.
[30] Leidy, H.J., Mattes, M.R. and Campbell, W.W. (2007) Effects of acute and chronic protein intake on metabolism, appetite, and ghrelin during weight loss. Obesity, 15, 1215-1225.
[31] Salbe, A.D., Tshop, M.H., Delparigi, A., Venti, C. and Tataranni, P.A. (2004) Negative relationship between fasting plasma ghrelin concentrations and ad libitum food intake. The Journal of Clinical Endocrinolology and Metabolism, 89, 2951-2956.
[32] Erdmann, J., Topsch, R., Lippl, F., Gussmann, P. and Schusdziarra, V. (2004) Postprandial response of plasma ghrelin levels to various test meals in relation to food intake, plasma insulin, and glucose. The Journal of Clinical Endocrinolology and Metabolism, 89, 3048-3054.
[33] Reinehr, T., Roth, C., Menke, T. and Andler, W. (2004) Adiponectin before and after weight loss in obese children. The Journal of Clinical Endocrinolology and Metabolism, 89, 3790-3794.
[34] Cambuli, V.M., Musiu, M.C., Incan, M., et al. (2008) Assessment of adiponectin and leptin as biomarkers of positive metabolic outcomes after lifestyle intervention in overweight and obese children. The Journal of Clinical Endocrinolology and Metabolism, 93, 3051-3057.
[35] Martos-Moreno, G.A., Barrios, V., Martínez, G., Hawkins, F. and Argente, J. (2010) Effect of weight loss on high-molecular weight adiponectin in obese children. Obesity, 18, 2288-2294.
[36] Mauras, N. et al. (2008) Adiponectin and catecholamine concentrations during acute exercise in children with type 1 diabetes. Pediatric Diabetes, 9, 221-227.
[37] Kelly, A.S., Steinberger, J., Olson, T.P. and Dengel, D.R. (2007) In the absence of weight loss, exercise training does not improve adipokines or oxidative stress in overweight children. Metabolism: Clinical and Experimental, 56, 1005-1009.
[38] Beltowski, J. (2003) Adiponectin and resistin: New hormones of white adipose tissue. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 9, 55-61.
[39] Bruun, J.M., Lihn, A.S., Verdich C., et al. (2003) Regulation of adiponectin by adipose tissue-derived cytokines: In vivo and in vitro investigations in humans. American Journal of Physiology. Endocrinology and Metabolism, 285, 527-533.
[40] Ronti, T., Lupattelli, G. and Mannarino, E. (2006) The endocrine function of adipose tissue: An update. Clinical Endocrinology, 64, 355-365.
[41] Jiang, J., Xia, X., Greiner, T., Wu, G., Lian, G. and Rosenqvist, U. (2007) The effects of a 3-year obesity intervention in schoolchildren in Beijing. Child: Care, Health and Development, 33, 641-646.
[42] Trinh, A., Campbell, M., Ukoumunne, O.C., Gerner, B. and Wake, M. (2013) Physical activity and 3-year BMI change in overweight and obese children. Pediatrics, 131, e470-e477.
[43] Snethen, J.A., Broome, M.E. and Cashin, S.E. (2006) Effective weight loss for overweight children: A meta-analysis of intervention studies. Journal of Pediatric Nursing, 21, 45-66.
[44] American College of Sports Medicine (2006) Guidelines for exercises testing and prescription. 7th Edition, Lippincott Williams & Wilkins, Philadelphia.
[45] Hill, J.O. and Wyatt, H.R. (2005) Role of physical activity in preventing and treating obesity. Journal of Applied Physiology, 99, 765-770.
[46] Physical Activity Guidelines Advisory Committee (2008) Physical activity guidelines advisory committee report. Department of Health and Human Services, Washington DC.
[47] Baumer, J.H. (2007) Guideline review: Obesity and overweight: Its prevention, identification, assessment and management. Archives of Disease in Childhood. Education Practice edition, 92, 92-96.
[48] Strong, W.B., Malina, R.M., Blimkie, C.J.R., et al. (2005) Evidence based physical activity for school-age youth. Journal of Pediatric, 146, 732-737.
[49] Andersen, L., Harro, M., Sardinha, L., et al. (2006) Physical activity and clustered cardiovascular risk in children: A cross sectional study—The european youth heart study. Lancet, 368, 299-304.
[50] Nowicka, P. and Flodmark, C.E. (2006) Physical activitykey issues in treatment of childhood obesity. Acta Paediatrica Supplement, 96, 39-45.
[51] Martins, C., Morgan, L. and Truby, H. (2008) A review of the effects of exercise on appetite regulation: An obesity perspective. International Journal of Obesity, 32, 1337-1347.

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