Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Entrapped in Titania Sol-Gel Film on Screen-Printed Electrode

Reza E. Sabzi, Fereshteh Rasouli, Farshad Kheiri

Department of Chemical Engineering, Urmia University of Technology, Urmia, Iran.
Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran.
Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran Institute of Biotechnology, Urmia University, Urmia, Iran.

DOI: 10.4236/ajac.2013.411072                

Abstract

We report the fabrication of disposable and flexible Screen-Printed Electrodes (SPEs). This new type of screen-printed electrochemical platform consists of Ag nanoparticles (AgNPs) and graphite composite. For this purpose, silver nanoparticles were first synthesized by a chemical reduction method. The morphology and structure of the AgNPs were analyzed using a Scanning Electron Microscope (SEM) and UV-Visible spectroscopy. Graphite was chosen as the working electrode material for the fabrication of a thick-film. The fabrication of a screen-printed hydrogen peroxide biosensor consisting of three electrodes on a polyethylene terephthalate (PET) substrate was performed with a spraying approach (working, counter and reference: enzyme electrode, graphite, pseudo reference: Ag/AgCl). This biosensor was fabricated by immobilizing the peroxidase enzyme (HRP) in a Titania sol-gel membrane which was obtained through a vapor deposition method. The biosensor had electrocatalytic activity in the reduction of H₂O₂ with linear dependence on H₂O₂ concentration in the range of 10^－5 to 10^－3 M; the detection limit was 4.5 × 10^－6 M.

Keywords

Screen-Printed Electrode; Ag Nanoparticle; Titania Sol-Gel; Biosensor

Conflicts of Interest

The authors declare no conflicts of interest.

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