Physiological Fluid Specific Agglomeration Patterns Diminish Gold Nanorod Photothermal Characteristics


Investigations into the use of gold nanorods (Au-NRs) for biological applications are growing exponentially due to their distinctive physicochemical properties, which make them advantageous over other nanomaterials. Au-NRs are particularly renowned for their plasmonic characteristics, which generate a robust photothermal response when stimulated with light at a wavelength matching their surface plasmon resonance. Numerous reports have explored this nanophotonic phenomenon for temperature driven therapies; however, to date there is a significant knowledge gap pertaining to the kinetic heating profile of Au-NRs within a controlled physiological setting. In the present study, the impact of environmental composition on Au-NR behavior and degree of laser actuated thermal production was assessed. Through acellular evaluation, we identified a loss of photothermal efficiency in biologically relevant fluids and linked this response to excessive particle aggregation and an altered Au-NR spectral profile. Furthermore, to evaluate the potential impact of solution composition on the efficacy of nano-based biological applications, the degree of targeted cellular destruction was ascertained in vitro and was found to be susceptible to fluid-dependent modifications. In summary, this study identified a diminution of Au-NR nanophotonic response in artificial physiological fluids that translated to a loss of application efficiency, pinpointing a critical concern that must be considered to advance in vivo, nano-based bio-applications.

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Comfort, K. , Speltz, J. , Stacy, B. , Dosser, L. and Hussain, S. (2013) Physiological Fluid Specific Agglomeration Patterns Diminish Gold Nanorod Photothermal Characteristics. Advances in Nanoparticles, 2, 336-343. doi: 10.4236/anp.2013.24046.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] M. C. Daniel and D. Astruc, “Gold Nanoparticles: Assembly, Supramolecular Chemistry, Quantum-Size-Related Properties, and Applications Toward Biology, Catalysis, and Nanotechnology,” Chemical Reviews, Vol. 104, No. 1, 2004, pp. 293-346.
[2] L. Dykman and N. Khlebtsov, “Gold Nanoparticles in Biomedical Applications: Recent Advances and Perspectives,” Chemical Society Reviews, Vol. 41, No. 6, 2012, pp. 2256-2282.
[3] Y. Wang, X. Xie, X. Wang, G. Ku, K. L. Gill, D. P. O’Neal, G. Stoica and L. V. Wang, “Photoacoustic Tomography of a Nanoshell Contrast Agent in the in Vivo Rat Brain,” Nano Letters, Vol. 4, No. 9, 2004, pp. 1689-1692.
[4] E. C. Dreaden, A. M. Alkilany, X. Huang, C. J. Murphy and M. A. El-Sayed, “The Golden Age: Gold Nanoparticles for Biomedicine,” Chemical Society Reviews, Vol. 41, No. 7, 2012, pp. 2740-2779.
[5] X. Huang, I. H. El-Sayed, W. Qian and M. A. El-Sayed, “Cancer Cell Imaging and Photothermal Therapy in the Near-Infrared Region by Using Gold Nanorods,” Journal of the American Chemical Society, Vol. 128, No. 6, 2006, pp. 2115-2120.
[6] H. H. Richardson, M. T. Carlson, P. J. Tandler, P. Hernandez and A. O. Govorov, “Experimental and Theoretical Studies of Light-to-Heat Conversion and Collective Heating Effects in Metal Nanoparticle Solutions,” Nano Letters, Vol. 9, No. 3, pp. 1139-1146.
[7] A. M. Alkilany, L. B. Thompson, S. P. Boulos, P. N. Sisco and C. J. Murphy, “Gold Nanorods: Their Potential for Photothermal Therapeutics and Drug Delivery, Tempered by the Complexity of Their Biological Interactions,” Advanced Drug Delivery Reviews, Vol. 64, No. 2, 2012, pp. 190-199.
[8] W. I. Choi, J. Kim, C. Kang, C. C. Byeon, Y. H. Kim and G. Tae, “Tumor Regression in Vivo by Photothermal Therapy Based on Gold-Nanorod-Loaded, Functional Nanocarriers,” ACS Nano, Vol. 5, No. 3, 2011, pp. 1995-2003.
[9] T. S. Hauck, T. L. Jennings, T. Yatsenko, J. C. Kumaradas and W. C. W. Chan, “Enhancing the Toxicity of Cancer Chemotherapeutics with Gold Nanorod Hyperthermia,” Advanced Materials, Vol. 20, No. 20, 2008, pp. 3832-3838.
[10] J. Huang, K. S. Jackson and C. J. Murphy, “Polyelectrolyte Wrapping Layers Control Rates of Photothermal Molecular Release from Gold Nanorods,” Nano Letters, Vol. 12, No. 6, 2012, pp. 2982-2987.
[11] S. Link, M. B. Mohamed and M. A, El-Sayed, “Simulation of the Optical Absorption Spectra of Gold Nanorods as a Function of their Aspect Ratio and the Effect of the Medium Dielectric Constant,” Journal of Physical Chemistry B, Vol. 103, No. 16, 1999, pp. 3073-3077.
[12] J. C. Y. Kah, A. Zubieta, R. A. Saavedra and K. HamadSchifferli, “Stability of Gold Nanorods Passivated with Amphiphilic Ligands,” Langmuir, Vol. 28, No. 24, 2012, pp. 8834-8844.
[13] M. Sethi, G. Joung and M. R. Knecht, “Stability and Electrostatic Assembly of Au Nanorods for Use in Biological Assays,” Langmuir, Vol. 25, No. 1, pp. 317-325.
[14] P. K. Jain, K. S. Lee, I. H. El-Sayed and M. A. El-Sayed, “Calculated Absorption and Scattering Properties of Gold Nanoparticles of Different Size, Shape, and Composition: Applications in Biological Imaging and Biomedicine,” Journal of Physical Chemistry B, Vol. 110, No. 14, 2006, pp. 7238-7248.
[15] B. M. Stacy, K. K. Comfort, D. A. Comfort and S. M. Hussain, “In Vitro Identification of Gold Nanorods through Hyperspectral Imaging,” Plasmonics, Vol. 8, No. 2, 2013, pp. 1235-1240.
[16] K. K. Comfort, E. I. Maurer, L. K. Braydich-Stolle and S. M. Hussain, “Interference of Silver, Gold, and Iron Oxide Nanoparticles on Epidermal Growth Factor Signal Transduction in Epithelial Cells,” ACS Nano, Vol. 5, No. 12, 2011, pp. 10000-10008.
[17] M. C. DeBrosse, K. K. Comfort, E. A. Untener, D. A. Comfort and S. M. Hussain, “High Aspect Ratio Gold Nanorods Displayed Augmented Cellular Internalization and Surface Chemistry Mediated Cytotoxicity,” Materials Science and Engineering C, Vol. 33, No. 7, 2013, pp. 4094-4100.
[18] K. Park and R. A. Vaia, “Synthesis of Complex Au/Ag Nanorods by Controlled Overgrowth,” Advanced Materials, Vol. 20, No. 20, 2008, pp. 3882-3886.
[19] K. Park, H. Koerner and R. A. Vaia, “Depletion-Induced Shape and Size Selection of Gold Nanoparticles,” Nano Letters, Vol. 10, No. 4, 2010, pp. 1433-1439.
[20] Y, Liu, M. K. Shipton, J. Ryan, E. D. Kaufman, S. Frazen and D. L. Feldheim, “Synthesis, Stability, and Cellular Internalization of Gold Nanoparticles Containing Mixed Peptide-Poly(ethylene glycol) Monolayers,” Analytical Chemistry, Vol. 79, No. 6, 2007, pp. 2221-2229.
[21] W. Stopford, J. Turner, D. Cappellini and T. Brock, “Bioaccessibility Testing of Cobalt Compounds,” Journal of Environmental Monitoring, Vol. 5, No. 4, 2003, pp. 675-680.
[22] J. Alper and K. Hamad-Schifferli, “Effect of Ligand on Thermal Dissipation from Gold Nanorods,” Langmuir, Vol. 26, No. 6, 2010, pp. 3786-3789.
[23] A. M. Alkilany, P. K. Nagaria, C. R. Hexel, T. J. Shaw, C. J. Murphy and M. D. Wyatt, “Cellular Uptake and Cytotoxicity of Gold Nanorods: Molecular Origin of Cytotoxicity and Surface Effects,” Small, Vol. 5, No. 6, 2009, pp. 701-708.
[24] V. P. Pattani and J. W. Tunnell, “Nanoparticle-Mediated Photothermal Therapy: A Comparative Study of Heating for Different Particle Types,” Lasers in Surgery and Medicine, Vol. 44, No. 8, 2012, pp. 675-684.
[25] L. V. Stebounova, E. Guio and V. H. Grassian, “Silver Nanoparticles in Simulated Biological Media: A Study of Aggregation, Sedimentation, and Dissolution,” Journal of Nanoparticle Research, Vol. 13, No. 1, 2011, pp. 233-244.
[26] I. Lynch, A. Salvati and K. A. Dawson, “Protein-Nanoparticle Interactions: What Does the Cell See?” Nature Nanotechnology, Vol. 4, No. 9, 2009, pp. 546-547.

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