Formaldehyde Biosensor with Formaldehyde Dehydrogenase Adsorped on Carbon Electrode Modified with Polypyrrole and Carbon Nanotube

Mingwei Wang, Shuhai Jiang, Yi Chen, Xiang Chen, Li Zhao, Juankun Zhang, Jinfeng Xu

Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin Key Laboratory of Industry Microbiology, College of Biotechnology, Tianjin University of Science&Technology, Tianjin, 300457, China.

DOI: 10.4236/eng.2012.410B035                

Abstract

In this study, a carbon electrode electroless polymerization of polypyrrole, which formed a layer of conductive film interface,then absorped a layer of carbon nanotube particles by the way of self-assembled, is studied. The modified electrode is used to electrostatic adherence of formaldehyde dehydrogenase, with Nafion solution capped. Moreover this paper discusses the work of electrode position,  PH value and scanning speed on the influence of the electrode response, the response potential is -1.2v, the optimal PH value is 7.5, and we also review the linear range of this electrode, we discovered taht this biosensor have a good linear relationship with the concentration of 1ug/ml-360ug/ml, the Correlation coefficient is 0.9983. In addition,with the stability of this electrode tested, it can be stored 15d at4°C.

Keywords

Formaldehyde Dehydrogenase; Eletrostatic Self-assembly; Biosensor

Conflicts of Interest

The authors declare no conflicts of interest.

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