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Formaldehyde Biosensor with Formaldehyde Dehydrogenase Adsorped on Carbon Electrode Modified with Polypyrrole and Carbon Nanotube

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DOI: 10.4236/eng.2012.410B035    3,335 Downloads   4,314 Views   Citations

ABSTRACT

In this study, a carbon electrode electroless polymerization of polypyrrole, which formed a layer of conductive film interface,then absorped a layer of carbon nanotube particles by the way of self-assembled, is studied. The modified electrode is used to electrostatic adherence of formaldehyde dehydrogenase, with Nafion solution capped. Moreover this paper discusses the work of electrode position,  PH value and scanning speed on the influence of the electrode response, the response potential is -1.2v, the optimal PH value is 7.5, and we also review the linear range of this electrode, we discovered taht this biosensor have a good linear relationship with the concentration of 1ug/ml-360ug/ml, the Correlation coefficient is 0.9983. In addition,with the stability of this electrode tested, it can be stored 15d at4°C.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

M. Wang, S. Jiang, Y. Chen, X. Chen, L. Zhao, J. Zhang and J. Xu, "Formaldehyde Biosensor with Formaldehyde Dehydrogenase Adsorped on Carbon Electrode Modified with Polypyrrole and Carbon Nanotube," Engineering, Vol. 4 No. 10B, 2012, pp. 135-138. doi: 10.4236/eng.2012.410B035.

References

[1] JIANG Zhongfa, YUAN Wenjun, ZHANG Benyan. Progress of study on the relationship between Indoor air formaldehyde Pollution and Leukemia[J] , Journal of Environment and Health, 2008,25(3): 276-278 (in Chinese).
[2] ZHAI, Jinxia, ZHANG Chuanmu, ZHANG Peng. Acute effects of formaldehyde in anatomy laboratory on student health[J]. Journal of Environment and Health, 2008,25(2): 141-143 (in Chinese).
[3] Yang J,Zhang B,et a1.Cloning and Expression of Pseudomonas fluorescens 26-2 lipase gene in Pichla paaoris and characterizing for transosterification.Appl Biochem Biotechnol,2008,159(2):355-365.
[4] Mitsubayashi K, NishioG, SawaiM, A bio-sniffer stick with FALDH (formaldehyde dehydrogenase)for convenient analysis of gaseous formaldehyde[J] . Sensors and ActuatorsB, 2008, 130( 1):32~ 37.
[5] Boukerma K,Piquemal J Y,Chehimi M M,et al.Synthesis and interfacial properties of montmorillo-nite/polypyrrolenano- composites[J].Polym.,2006,47:569-576.
[6] Ren X Z,Zhao Q,Liu J H,et al.Preparation of polypyrrole nanop-articles in reverse micelle anditsapplication to glucose biosensor[J].Journal of Nanoscience and Nanotechnology,doi:10.1166/jnn.2007(2):141.
[7] Wang S G,Zhang Q,Wang R L,et al.A Novel multi-walled carbonnanotube-based biosensor for glucose detection[J].Biochem BiophysRes Commun,2003,311(3):572~576.
[8] ZHANG Shu-ping, ZHAO Yan, MAJie1, LIUXiao-hui, WANGMeng-fei, LIUWei. Study on performance of layer-by-layer self-assembled electrodein detection of thiocholine[J].Modern Chemical Industry,2011,Jan,31(1):88~92
[9] William G.Gutheil, Elvin Kasimoglu, and Permila C.Nicholson. Induction of Glutathione-Dependent Formaldehyde Dehydrogenase Activity in Escherichia coli and Hemophilus influenza[J]. Biochemical and biophysical research communications 238, 693--696 (1997)
[10] William G.Gutheil,Barton Holmquist,and Bert L.Vallee. Purification, Characterization, and Partial Sequence of the Glutathione-Dependent Formaldehyde Dehydrogenase from Escherichia coli: A Class I11 Alcohol[J]. Biochemistry 1992, 31, 475-481.
[11] Shi yintao,Yuan ruo,Wang na, The biosensor based on polythionine Nano Au and horseradish peroxidase modified for detecting Hydrogen peroxide[J], Southwest China Normal University,2006,31,3.
[12] hou guoqing, The biosensor based on polyvinylpyrrolidone/Nano Au and horseradish peroxidase modified for detecting Hydrogen peroxide[J], Southwest China Normal University,2010,36,4.
[13] M. Ben Ali, M. Gonchar, G. Gayda, S. Paryzhak, M.A. Maaref, N. Jaffrezic-Renault, Y. Korpan . Formalde-hyde-sensitive sensor based on recombinant formaldehydedehydrogenase using capacitance versus voltage measurements[J]. Biosensors and Bioelectronics 22 (2007) 2790–2795
[14] Kohji Mitsubayashi, Genki Nishio, Masayuki Sawai, Elito Kazawa . A biochemical sniffer-chip for convenient analysis of gaseousformaldehyde from timber materials[J]. Microchim Acta (2008) 160: 427–433.
[15] Lilach Bareket, Ada Rephaeli, Gili Berkovitch, Abraham Nudelman, Judith Rishpon. Carbon nanotubes based electrochemical biosensor for detection of formaldehyde released from a cancer cell line treated with formaldehyde-releasing anticancer prodrugs[J]. Bioelectrochemistry 77 (2010) 94–99.
[16] Yaroslav I. Korpan & Olexandr O. Soldatkin & Olga F. Sosovska & Halyna M. Klepach . Formaldehyde-sensitive conductometric sensors based on commercial and recombinant formaldehyde dehydrogenase[J]. Microchim Acta (2010) 170:337–344.

  
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