MS-HRM to Detect Serum DNA Methylation of Intrauterine Growth Retardation Children

Yan Du, Youxia Zhou, Qian Wu

Department of Pathology, Maternity and Children's Health Care Centers in Wuxi, Wuxi, China.
Research Center of Learning Science, Southeast University, Nanjing, China.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.

DOI: 10.4236/eng.2012.410B027                

Abstract

Intrauterine growth retardation (IUGR) is also called fetal growth restriction (FGR), which is the major complications in obstetrics and one of the most important causation from high morbidity and mortality in perinatal. In this study, MS-HRM (Methylation-Sensitive High Resolution Melting Curve Analysis) was used to detect the methylation status of serum DNA. Gene of insulin-like growth factor binding protein 3 (IGFBP-3) was detected in this study. Results showed that the serum DNA methylation level of FGR fetus were lower than that of the control group; different methylation levels were also found between male and female fetus; and the methylation level was increased with the birth weight of the newborn. Our results showed that the IGFBP-3 gene methylation level of serum DNA of newborn could be semi-quantitative detected which guide the early prevention and treatment of IUGR. It also indicated that the methylation status of serum DNA can be conveniently identified and quantified by inspection of the melting curves.

Keywords

MS-HRM; IUGR; IGFBP-3; Serum DNA

Conflicts of Interest

The authors declare no conflicts of interest.

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