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How can aprotic ionic liquids affect enzymatic enantioselectivity?

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DOI: 10.4236/jbise.2013.610117    3,054 Downloads   4,492 Views   Citations
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Hidetaka Noritomi, Hideki Chiba, Manabu Kikuta, Satoru Kato


Dai-Ichi Kogyo Seiyaku Co., Ltd., Oogawara-cho, Kisshoin, Kyoto, Japan.
Department of Applied Chemistry, Tokyo Metropolitan University, Tokyo, Japan.


The enantioselectivity of α-chymotrypsin in the esterification of N-acetyl-tryptophan with ethanol was examined in aprotic ionic liquids. The enantioselectivity was found to be strongly affected by a kind of solvent, water content, and reaction temperature. The (kcatKM)L/(kcat/KM)D ratio in 1-ethyl-3-methylimidazolium bis(fluorosulfonyl)imide ([C2mim][FSI]) containing 1.0% (v/v) water at 25°C exhibits 32,000, while that in 1-ethyl-3-methylimidazolium tetrafluoroborate ([C2mim][BF4]) containing 1.0% (v/v) water at 25°C shows 190. The (kcat/KM)L/(kcat/KM)D ratio in [C2mim] [BF4] at 25°C varies from 190 at 1.0% (v/v) water to 65000 at 5.0% (v/v) water. Moreover, the (kcat/KM)L/ (kcat/KM)


α-Chymotrypsin; Enantioselectivity; Esterification; Aprotic Ionic Liquid

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Noritomi, H. , Chiba, H. , Kikuta, M. and Kato, S. (2013) How can aprotic ionic liquids affect enzymatic enantioselectivity?. Journal of Biomedical Science and Engineering, 6, 954-959. doi: 10.4236/jbise.2013.610117.

Conflicts of Interest

The authors declare no conflicts of interest.


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