Isolation and Characterization of R-Enantiomer in Ezetimibe

Kameswararao Chimalakonda, Venugopal Kamani, Madhusudhan Gutta, Srinivasulu Polisetty, Sai Venkata Srinivas Koduri

Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India.

DOI: 10.4236/ajac.2013.49062                

Abstract

A simple and rapid Supercritical Fluid Chromatography (SFC) method has been developed to isolate and characterize R-Isomer of Ezetimi be by using normal phase Chiral Cel OD-H with 250 mm × 30 mm, 5 microns column using a mobile phase system containing super critical fluid carbondi oxide (Co2) and the percentage of 2-Propanol as a mobile phase (85:15) and detection at 230 nm. The isolated R-Isomer is characterized by using UV-vis, FT-IR, ESI-MS, HPLC¹H and ¹³C NMR. The purity of isolated R-Isomer is about 98%.

Keywords

Isolation; Characterization; (R)-Isomer; Ezetimibe; Supercritical Fluid Hromatography (SFC)

Conflicts of Interest

The authors declare no conflicts of interest.

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