Pressurized Hot Water Extraction of Alkaloids in Goldenseal

Janes Mokgadi, Charlotta Turner, Nelson Torto

Department of Chemistry, Lund University, Centre for Analysis and Synthesis, Lund, Sweden.
Department of Chemistry, Rhodes University, Grahamstown, South Africa.

DOI: 10.4236/ajac.2013.48050                

Abstract

A quick, efficient and environmentally beneficial approach for the extraction of alkaloids in goldenseal based on pressurized hot water extraction (PHWE) is presented. PHWE was evaluated following the extraction of hydrastine and berberine which are the two main alkaloids of goldenseal. The effects of temperature and other parameters on extraction yields were monitored by high performance liquid chromatography coupled to diode array detector (HPLC-DAD). The extraction yields were compared to those achieved through reflux and ultrasonic extraction methods. PHWE employed hot water at an optimal temperature of 140℃, a pressure of 50 bars and flow rate of 1 mL^.min^-1 for extraction experiments conducted in the dynamic mode. Both PHWE and conventional methods achieved comparable extraction yields. Reflux and ultrasonic extractions had procedures that were slower (over 6 h) and employed large quantities (200 mL) of organic solvents. The PHWE method was simple and relatively fast as extraction was achieved in 15 min. At 95% confidence level (n = 3), reflux and ultrasonic methods recorded extraction yields that were not significantly different from that of PHWE. Moreover, PHWE employed a more readily available “green” solvent, (water) to achieve comparable results to those of conventional methods which utilized organic solvents. The percentage relative standard deviations (%RSD) for the extraction yields were less than 3% in all cases indicating the high reproducibility of the method. However, when using PHWE to extract thermally labile analytes like alkaloids, degradation effects also occurred. Increasing the temperatures certainly achieved higher extraction yields but also led to degradation of these thermally labile compounds indicating a necessity for optimization.

Keywords

Subcritical Water Extraction; Extraction Yields; Degradation; Hydrastine; Berberine

Conflicts of Interest

The authors declare no conflicts of interest.

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