Determination of caprine serum albumin in milk using bromocresol green dye

DOI: 10.4236/ojas.2013.33039   PDF   HTML     5,445 Downloads   8,079 Views   Citations

Abstract

The objective of this study was to develop and validate a colorimetric assay to measure caprine serum albumin (CSA) concentrations in milk. The principle of the say was based on the binding of albumin to bromocresol green (BCG) dye at pH 4.0. The absorption of the dye-albumin complex was determined spectrophotometrically at 640 nm. A linear relationship existed between CSA concentrations (0.01 to 8.0 mg/ml) and absorbance values. Intra-assay and interassay coefficients of variation for the proposed assay were 2.6% and 12% respectively. Recovery from milk samples spiked with albumins was 89.15% ± 7.6%. The developed assay was validated using 126 milk samples collected from lactating Saanen goats at various stages of lactation`. Caprine serum albumin concentrations in milk ranged between 0.31 and 0.52 mg/ml. The concentrations of CSA were significantly higher (p < 0.05) at the beginning of lactation, declined rapidly and remained relatively stable in mature milk. Caprine serum albumin correlated positively (p < 0.05) with milk somatic cell counts, total solids, protein, fat and whey and correlated negatively (p < 0.05) with milk lactose and casein concentrations. It was concluded that the proposed BCG dye-binding method is easy to perform, rapid, sensitive, reliable and can be used to determine CSA concentrations in milk collected from goats under different physiological conditions.

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Olaniyan, B. , Zhao, X. and Mustafa, A. (2013) Determination of caprine serum albumin in milk using bromocresol green dye. Open Journal of Animal Sciences, 3, 261-267. doi: 10.4236/ojas.2013.33039.

Conflicts of Interest

The authors declare no conflicts of interest.

References

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