Structure of vitreous body and its relationship with liquefaction

Makoto Kodama, Toyoaki Matsuura, Yoshiaki Hara

Department of Ophthalmology, Nara Medical University, Nara, Japan.
Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan.

DOI: 10.4236/jbise.2013.67091                

Abstract

The aim of the study is to clarify the vitreous body structure and liquefaction phenomena. It was found that when melting a frozen rabbit vitreous body, the gel-sol transition phenomenon occurs and the gel structure is broken. This is almost like the liquefaction of the vitreous body in vivo. We try to clarify the liquefaction phenomenon by using this animal model. The native vitreous body has three dimensional meshwork structures. After liquefaction, it is changed into two parts, namely fiber aggregates and soluble amorphous aggregates. The surface of native vitreous body meshwork is mucopolysacharide, but that of fiber aggregates after liquefaction is changed into connective tissue, which means the conformational change of vitreous body in liquefaction. The soluble proteins after liquefaction were analyzed and identified as crystallin family. It is suggested that the liquefaction is induced by detachment of non-collagenous protein beads containing crystallins, resulting in the collapse of the three dimensional structure to release watery liquid trapped within. And the new gel-sol transition model of vitreous bod is proposed.

Keywords

Vitreous Body; Liquefaction; Crystallin

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Sebag, J. (2009) Vitreous: The resplendent enigma. British Journal of Ophthalmology, 93, 989-991. doi:10.1136/bjo.2009.157313
[2]	Berman, E.R. (1991) Vitreous. In: Graymore, C.N., Ed., Biochemistry of the Eye, Plenum Press, New York, 291-307
[3]	Balazs, E.A. (1961) Molecular morphology of the vitreous body. In: Smelser, G.K., Ed., The Structure of the Eye, Academic Press, New York, 293-310.
[4]	Davison, P.F. and Seery, C.M. (1993) An analysis of two fibril systems in the mammalian vitreous. Current Eye Research, 12, 107-114 doi:10.3109/02713689308999478
[5]	Worst, J.G.F. and Losm L.I. (1999) Cisternal anatomy of the vitreous. Kugler Publications, Amsterdam & New York.
[6]	Brewton, R.G. and Mayne, R. (1992) Mammalian vitreous humor contains networks of hyaluronan molecules. Experimental Cell Research, 198, 237-249. doi:10.1016/0014-4827(92)90376-J
[7]	Sebag, J. and Balazs, E.A. (1985) Human vitreous fibers and vitreoretinal disease. Transaction of the Ophthalmological Society of the United Kingdom, 104, 123-127. http://highwire.stanford.edu/cgi/medline/pmid;3857770?maxto
[8]	Kodama, M., Hayashi, M., Ishii, M., Fujii, N., Matsuura, T. and Hara, Y. (2010) Structure of vitreous body—Approach to liquefaction using animal model. Folia Japon ica de Ophthalmologica Clinica (in Japanese), 3, 1094-1100.
[9]	Kobayashi, A. (1992) The preparation method for dog eye ball specimen. Jikken Byorisosiki Gijyutu Kenkyu Kaishi (in Japanese), 1, 8-102.
[10]	Mallory, F.B. (1938) Pathological technique. W. B. Saunders, Philadelphia.
[11]	Mowry, R.W. (1963) The special value of methods that color both acidic and vicinal hydroxyl groups in the histochemical study of mucins withrevised directions for the colloidal iron stain, the use of alcian blue G8X and their combinations with the periodic acid-schiff reaction. Annals of the New York Academy of Sciences, 402-423.
[12]	Curtis, F. (1905) Methode de colorationelective du tissue conjonctif. C.R. soc. Biol., 58, 1038-1040.
[13]	Asakura, A. (1985) Histochemistry of hyaluronic acid of the bovine vitreous body as studied by electron micros copy. Acta Society of Ophthalmology of Japan, 89, 179-191
[14]	Scott, J.E. (1992) The chemical morphology of the vitreous. Eye, 6, 553-555. doi:10.1038/eye.1992.120
[15]	Bishop, P.N., Holmes, D.F., Kadler, K.E., et al. (2004) Age-related changes on the surface of vitreous collagen fibrils. Investigative Ophthalmology and Visual Science, 45, 1041-1046. doi:10.1167/iovs.03-1017
[16]	Los, L.I., vander Worp, R.J., van Luynm, M.J.A., et al. (2003) Age-related liquefaction of the human vitreous body: LM and TEM evaluation of the role of proteogly cans and collagen. Investigative Ophthalmology and Visual Science, 44, 2828-2833. doi:10.1167/iovs.02-0588
[17]	van Deemer, M., Pas, H.H., Kuijer, R., et al. (2009) En zymatic breakdown of type 2 collagen in the human vit reous. Investigative Ophthalmology and Visual Science, 50, 4552-4560. doi:10.1167/iovs.08-3125
[18]	Lovicu, F.J., Chamberlain, C.G. and McAvoy, J.W. (1995) Differential effects of aqueous and vitreous on fiber differentiation and extracellular matrix accumulation in lens epithelial explants. Investigative Ophthalmology and Visual Science, 36, 1459-1469.
[19]	Ponsioen, T.L., van Deemer, M., Bank, R.A., et al. (2009) Mature enzymatic collagen cross-links, hydroxylsylpyri dinoline and lysylpyridinoline, in the aging human vitreous. Investigative Ophthalmology and Visual Science, 50, 1041-1045. doi:10.1167/iovs.08-1714
[20]	Grandofer, A. (2008) Enzymatic vitreous disruption. Eye, 22, 1273-1279. doi:10.1038/eye.2008.29
[21]	Hikichi, T., Ueno, N., Chakrabarti, B. and Trempe, C.L. (1996) Vitreous changes during ocular inflammation in duced by interleukin 13. Japanese Journal of Ophthal mology, 40, 297-302
[22]	Akiba, J., Ueno, N. and Chakrabarti, B. (1994) Mechanism of photo-induced vitreous liquefaction. Current Eye Research, 13, 505-512. doi:10.3109/02713689408999882
[23]	Ponsioen, T.L., Hooymans, J.M.M. and Los, L.I. (2010) Remodelling of the human vitreous and vitreoretinal interface—A dynamic Process. Progress Retinal Eye Research, 29, 580-595. doi:10.1016/j.preteyeres.2010.07.001
[24]	Nishikawa, S. and Tamai, M. (1996) Ultrastructure of hyaluronic acid and collagen in the human vitreous. Current Eye Research, 15, 37-43. doi:10.3109/02713689609017609
[25]	Balazs, E.A. and Denlinger, J.L. (1984) The vitreous. In: Davison, Ed., The Eye, Academic Press, London, 533 589.
[26]	Fujii, N., Satoh, K.H. and Ishibashi, Y. (1994) Simultaneous stereoinversion and isomerization at specific aspartic acid residues in a A-crystallin from human lens. Journal of Biochemistry, 116, 663-669.
[27]	Pal, J., Bera, S. and Ghosh, S.K. (1999) Acetylation of α crystallin with N-acetylimidazole and its influence upon the native aggregate and subunit reassembly. Current Eye Research, 19, 358-367.
[28]	Srinivasan, A.N., Nagineni, C.N. and Bhat, S.P. (1992) A-crystallin is expressed in non-ocular tissues. Journal of Biological Chemistry, 267, 23337-23342
[29]	Yamane, K., Minamoto, A., Yamashita, H., Takamura, H., Miyamoto-Myoken, Y., Yoshizato, K., Nabetani, T., Tsu gita, A. and Mishima, H. (2003) Proteome analysis of human vitreous proteins. Molecular & Cellular Proteomics, 2, 1177-1187.
[30]	Bahk, S.C., Jang, J.U., Chai, C.U., Lee, S.H., Park, Z.Y., Yang, J.Y., Kim, J.D., Yang, Y.S. and Chung, H.T. (2007) Post-translational modeification of crystallins in vitreous body from experimental autoimmune uveitis of rats. Journal of Proteome Research, 6, 3891-3898. doi:10.1021/pr070133k
[31]	Tamburro, D., Facchiano, F., Petricoin, E.F., Liotta, L.A. and Zhou, W. (2010) Mass spectrometry-based characterization of the vitreous phophoproteome. Proteomics Clinical Application, 4, 839-846.
[32]	Linklater, H.A., Dzialoszynski, T., MaLeod, H.L., Sanford, S.E. and Trevitrhick, J.R. (1992) Modeling cortical cataractogenesis XII: Supplemental vitamin a treatment reduces gamma crystallin leakage from lenses in diabetic rats. Lens and Eye Toxicity Research, 9, 115-126.
[33]	Lutyen-Drecoll, E., May, C.A., Polansky, J.R., Johnson, D.H., Bloemendal, H. and Nguyen, T.D. (1998) Localiza tion of the stress proteins y-crystallin and trabecular meshwork inducible glucocorticoid response protein in normal and glaucomatous trabecular meshwork. Investigative Ophthalmology and Visual Science, 39, 517-525.
[34]	Imai, H., Ohta, K., Yoshida, A., Suzuki, S., Hashizume, K., Usami, S. and Kikuchi, T. (2010) Crystallin, new candidate proteins in endotoxin-induced uveitis. Investigative Ophthalmology and Visual Science, 51, 3554. doi:10.1167/iovs.09-3728
[35]	Feener, E.P., Madigan, M.C., Provis, J.M., Gao, B., Sadun, A.A., Aiello, L.P. and Sebag, J. (2010) Comparative proteome analysis of the developing and adult human vitreous. Investigative Ophthalmology and Visual Science, 51, 5096.
[36]	Angi, M., Kalirai, H., Coupland, S.E., et al., (2012) Proteomic analyses of the vitreous humour. Mediations of Inflammation, 2012, 148039.