Measurement of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS)

Sungmin Hong, Ying-Nai Wang, Hirohito Yamaguchi, Harinibytaraya Sreenivasappa, Chao-Kai Chou, Pei-Hsiang Tsou, Mien-Chie Hung, Jun Kameoka

Department of Electrical and Computer Engineering, Texas A & M University, College Station, Texas, USA.
Department of Molecular and Cellular Oncology, University of Texas, M. D. Anderson Cancer Center, Houston, Texas, USA.
Department of Molecular and Cellular Oncology, University of Texas, M. D. Anderson Cancer Center, Houston, Texas, USA..

DOI: 10.4236/jbnb.2010.11004                

Abstract

We have applied Raster Image Correlation Spectroscopy (RICS) technique to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. HeLa cells expressing Green Fluores-cent Protein (GFP) tagged p53 were incubated with and without DNA damaging agents, cisplatin or eptoposide, which are widely used as chemotherapeutic drugs. Then, the diffusion coefficient of GFP-p53 was determined by RICS and it was significantly reduced after the drug treatment while that of the one without drug treatment was not. It is suggested that the drugs induced the interaction of p53 with either other proteins or DNA. Together, our results demonstrated that RICS is able to detect the protein dynamics which may be associated with protein-protein or protein-DNA interactions in living cells and it may be useful for the drug screening.

Keywords

Raster Image Correlation Spectroscopy, Diffusion Coefficient, p53, DNA Damage

Conflicts of Interest

The authors declare no conflicts of interest.

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