Anticancer Effect of Plant-Derived Polysaccharides on Mice


Tupistra chinensis is a widely used medicinal plant in Hubei Province,China. In our study, we investigated the effect of polysaccharides component from Tupistra chinensis on mice in vivo. The mice H22 hepatocarcinoma animal model was established firstly. Then, the mice were fed with different doses of polysaccharides derived from Tupistra chinensis (one time each day). The neoplasm growth condition was analyzed and histological and electron microscopic analyses were performed. Compared to the control, Tupistra chinensis significantly inhibited the neoplasm growth. Large mass necrosis in the mouse model was observed under the light microscope and the necrosis area was significantly increased in the Tupistra chinensis-treated mice. Electron microscopic observations showed that severe apoptosis occurred in the cancer tissue in the mouse model and apoptosis was also significantly increased in Tupistra chinensis-treated mice. Our study demonstrated that Tupistra chinensis could inhibit the growth of H22 hepatocarcinoma. The mechanisms could involve the inhibitory effects of Tupistra chinensis on apoptosis, although the precise mechanisms remained to be elucidated in future study.

Share and Cite:

W. Huang, "Anticancer Effect of Plant-Derived Polysaccharides on Mice," Journal of Cancer Therapy, Vol. 4 No. 2, 2013, pp. 500-503. doi: 10.4236/jct.2013.42061.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] W. B. Pan, L. M. Wei, L. L. Wei, et al., “Chemical Constituents of Tupistra chinensis Rhizomes,” Chemical and Pharmaceutical Bulletin (Tokyo), Vol. 54, No. 7, 2006, pp. 954-958. doi:10.1248/cpb. 54.954
[2] K. Zou, J. Z. Wang, M. Du, et al., “A Pair of Diastereoisomeric Steroidal Saponins from Cytotoxic Extracts of Tupistra chinensis Rhizomes,” Chemical and Pharmaceutical Bulletin (Tokyo), Vol. 54, No. 10, 2006, pp. 1440-1442. doi:10.1248/cpb.54.1440
[3] C. Yang, Z. Liu and K. Zou, “Anti-Cancer Research of Saponin from Tupistra chinensis Bak. in Vitro,” Lishizhen Medicine and Materia Medica Research, Vol. 20, No. 10, 2009, pp. 2390-2392.
[4] T. Okamoto, R. Kodoi, Y. Nonaka, et al., “Lentinan from Shiitake Mushroom (Lentinus edodes) Suppresses Expression of Cytochrome P450 1A Subfamily in the Mouse Liver,” Biofactors, Vol. 21, No. 1-4, 2004, pp. 407-409. doi:10.1002/biof.552210180
[5] B. Zhang, Y. Fang, J. Cao, et al., “Involvement of Immune Response in Anti-Tumor Effects of Staphylococcus aureus Filtrate Preparation,” Journal of Cancer Research and Clinical Oncology, Vol. 134, No. 3, 2008, pp. 373-379. doi:10.1007/s00432-007-0293-y
[6] Z. Zhu, C. Yu and J. Cai, “Anti-Cancer Research of Tupistra Chinensis Bak. Extract,” Journal of Chinese Medicinal Materials, Vol. 29, No. 3, 2006, pp. 277-278.
[7] S. Karnjanapratum and S. You, “Molecular Characteristics of Sulfated Polysaccharides from Monostroma nitidum and Their in Vitro Anticancer and Immunomodulatory Activities,” International Journal of Biological Macromolecules, Vol. 48, No. 2, 2011, pp. 311-318. doi:10.1016/j.ijbiomac. 2010.12.002
[8] W. Z. Lu, G. X. Geng, Q. W. Li, et al., “Anti-Tumor Activity of Polysaccharides Isolated from Patrinia scabra Bunge on U14 Cervical Carcinoma Bearing Mice,” The American Journal of Chinese Medicine, Vol. 37, No. 5, 2009, pp. 933-944. doi:10.1142/S0192415X09007429

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.