PCR Detection of the Three Neofabraea Pathogenic Species Responsible for Apple Bull’s Eye Rot

Abstract

Apple bull’s eye rot is caused by pathogenic Neofabraea species including N. malicorticis, N. perennans and N. alba. Fruits carrying this fungal quarantine disease are prohibited from entering China. The host plants for the pathogens include several Rosaceae fruits including apple and pear. Disease symptoms and pathogen morphology are often insufficient to determine the identity of the pathogen, particularly at the species level. In the current study, we analyzed the inter-species sequence variations in the β-tublin gene, and designed specific primers to allow PCR amplification of 554 bp fragments from pathogenic Neofabraea species. The PCR products were recovered and sequenced, and Blast search was conducted using the DNA sequences in the Genbank database. The results indicated precise PCR amplification of the target sequences from the host pathogen, which allowed unambiguous identification of the species.

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D. Cao, X. Li, J. Cao and W. Wang, "PCR Detection of the Three Neofabraea Pathogenic Species Responsible for Apple Bull’s Eye Rot," Advances in Microbiology, Vol. 3 No. 1, 2013, pp. 61-64. doi: 10.4236/aim.2013.31009.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] L. Childs, “The Relation of Woolly Apple Aphis to Perennial Canker Infection with Other Notes on the Disease,” The Valley Library, Corvallis, 1929.
[2] J. H. Cunnington, “Three Neofabraea Species on Pome Fruit in Australia,” Australia Plant Pathology, Vol. 33, No. , 2004, pp. 453-454. doi:10.1071/AP04034
[3] N. Sharon, de Jong, C. A. Levesque, J. M. Gerard, Verkley, et al., “Phylogenetic Relationships among Neofabraea Species Causing Tree Cankers and Bull’s-Eye Rot of Apple Based on DNA Sequencing of ITS Nuclear rDNA, Mitochondrial rDNA, and the b-Tubulin Gene,” Mycological Research, Vol. 105, No. 6, 2001, pp. 658-669. doi:10.1017/S0953756201003926
[4] G. J. M. Verkley, “A Monograph of the Genus Pezicula and Its Anamorphs,” Studies in Mycology, Vol. 44, 1999, pp. 1-180.
[5] G. Bompeix and D. Cholodowski-Faivre, “Thermotherapie et Produits Naturels, Unetechnologie Emergente,” L’arboriculture Frutiere, Vol. 542, No. 3, 2000, pp. 19-25.
[6] K. L. Edney, “Post-Harvest Pathology of Fruits and Vegetables,” In: C. Dennis, Ed., Top Fruit, Academic Press, New York, 1983, pp. 43-71.
[7] E. J. Guthrie, “The Occurrence of Pezicula alba sp. nov. and P. malicorticis, the Perfect States of Gloeosporium album and G. perennans, in England,” Transactions of the British Mycological Society, Vol. 42, No. 4, 1959, pp. 502-506. doi:10.1016/S0007-1536(59)80052-8
[8] A. L. A. Snowdon, “Color Atlas of Post-Harvest Diseases and Disorders of Fruits and Vegetables,” In: General Introduction and Fruits, CRC Press, Boca Raton, 1990, p. 320.
[9] W. F. Wang, J. Hu, Y. Y. Zhang, et al., “The First and Repetitious Interception in Guangdong Port of Bull’s Eye Rot on Apple from US,” Chinese Plant Quarantine, Vol. 24, No. 1, 2010, pp. 35-37.
[10] S. F. Altschul, T. L. Madden, A. A. Schaffer, et al., “Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs,” Nucleic Acids Research, Vol. 25, No. 17, 1997, pp. 3389-3402.

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