Study Bacteriocin Production and Optimization Using New Isolates of Lactobacillus spp. Isolated from Some Dairy Products under Different Culture Conditions

Hoda Mahrous, Abeer Mohamed, M. Abd El-Mongy, A. I. El-Batal, H. A. Hamza

Menoufiya University, Shibin El Kom, Egypt.
National Center for Radiation Research and Technology, Cairo, Egypt.

DOI: 10.4236/fns.2013.43045                

Abstract

Lactobacilli belong to the group of lactic acid bacteria (LAB), that have several distinguished abilities such as production of lactic acid, enzymes such as β-Galactosidase and natural antimicrobial substances called bacteriocins. Bacteriocin is a biopreservative agent potential of suppressing growth of some contaminant bacteria in food industry but its commercial availability is limited and costly. The study aimed to select isolates of Lactobacillus spp. potential for producing bacteriocins to suppress the growth of Escherichia coli ATCC 25922 and Bacillus subtilis NCIB3610, and to optimize the process of bacteriocin production. Results obtained in this study showed that L. acidophilus isolate CH1 was selected as the best candidate for bacteriocin among the four isolates that tested. The largest amounts of the bacteriocins were synthesized only in MRS medium was supplemented with K₂HPO₄ (1.0%), Tween 80 (1%), Beef extract (1%), glucose, cyctein and peptone extract (1%). The optimization of culture conditions for bacteriocin production areas showed that corn steep liquor medium was the best medium for all isolates against Bacillus subtilis while no effect was observed on Escherichia coli ATCC 25922 except when used MRS medium. The optimum conditions for bacteriocin production were pH 6.0, temperature 34?C with 4% Phenyl acetamide showing the greatest growth inhibition areas.

Keywords

Lactobacillus acidophilus; Lactic Acid Bacteria; Bacteriocins Production

Conflicts of Interest

The authors declare no conflicts of interest.

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