Plasma catalase, glutathione-s-transferase and total antioxidant activity levels of children with attention deficit and hyperactivity disorder

V. Kenan Çelik, Erdal Erşan, Serpil Erşan, Sevtap Bakır, Orhan Dogan

Department of Chemistry Engineering, School of Medicine, Cumhuriyet University, Sivas, Turkey.
Departments of Biochemistry, School of Medicine, Cumhuriyet University, Sivas, Turkey.
Departments of Clinical Psychiatry, School of Medicine, Cumhuriyet University, Sivas, Turkey.
Numune Hospital, Adana, Turkey.

DOI: 10.4236/abb.2013.42026                

Abstract

Objective: In this study, we plan to measure plasma Catalase (CAT), Antioxidant Activity (AOA) and Glu- tathione-S-Transferase (GST) levels to understand whether oxidative stress develops or not and whether or not the detoxification mechanism properly functions in children with Attention Deficit and Hyperactivity Disorder (ADHD) with unknown etiology and pathogenesis. Method and Results: Plasma CAT, AOA, and GST activities were spectrophotometrically measured in forty patients (average age 10.27 ± 2.54) and thirty-five (average age, 9.97 ± 2.59) healthy individuals as the control group. While the CAT activity showed no difference in the patient group (P > 0.05) compared to the control group, AOA and GST levels were found significantly meaningful (P = 0.001). Conclusion: In this pilot study ,the study shows that no oxidative stress develops in individuals with ADHD in high AOA and stable CAT activity, and that the de- toxification mechanism functions extremely in high GST activity. These findings need to be supported by other studies.

Keywords

ADHD; Catalase; Antioxidant Activity; Oxidative Stress; Glutathione-S-Transferase

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Young, I.S. and Woodside, J.V. (2001) Antioxidants in health and disease. Journal of Clinical Pathology, 54, 176186. doi:10.1136/jcp.54.3.176
[2]	Change, B., Sies, H. and Baveris, A. (1979) Hydroperoxide metabolism in mammalian organs. Physiological Reviews, 59, 527-605.
[3]	Yu, B.P. (1994) Cellular defenses from damage from reactive oxygen species. Physiological Reviews, 74, 139-162.
[4]	Reiter, R.J. (1995) Oxidative processes and antioxidative defense mechanism. FASEB Journal, 9, 526-533.
[5]	Faraone, S.V., Sergeant, J., Gillberg, C. and Biederman, J. (2003) The worldwide prevalence of ADHD: Is it an American condition? World Psychiatry, 2, 104-113.
[6]	Biederman, J. and Faraone, S.V. (2005) Attention-deficit hyperactivity disorder. Lancet, 366, 237-248. doi:10.1016/S0140-6736(05)66915-2
[7]	Wolraich, M.L., Wibbelsman, C.J., Brown, T.E., Evans, S.W., Gotlieb, E.M., Knight, J.R., et al. (2005) Attentiondeficit/hyperactivity disorder among adolescents: A review of the diagnosis, treatment, and clinical implications. Pediatrics, 115, 1734-1746. doi:10.1542/peds.2004-1959
[8]	American Psychiatric Association (2005) Diagnostic and statistical manual of mental disorders. American Psychiatric Association, Washington DC.
[9]	Biederman, J., Petty, C.R., Evans, M., Small, J. and Faraone, S.V. (2010) How persistent is ADHD? A controlled 10-year follow-up study of boys with ADHD. Psychiatry Research, 177, 299-304. doi:10.1016/j.psychres.2009.12.010
[10]	Conners, C.K., Epstein, J.N., March, J.S., Angold, A., Wells, K.C., Klaric, J., et al. (2001) Multimodal treatment of ADHD in the MTA: An alternative outcome analysis, Journal of the American Academy of Child & Adolescent Psychiatry, 40, 159-167. doi:10.1097/00004583-200102000-00010
[11]	Faraone, S.V., Perlis, R.H., Doyle, A.E., Smoller, J.W., Goralnick, J.J., Holmgren, M.A., et al. (2005) Molecular genetics of attention-deficit/hyperactivity disorder. Biological Psychiatry, 57, 1313-1323. doi:10.1016/j.biopsych.2004.11.024
[12]	Khan, S.A. and Faraone, S.V. (2006) The genetics of attention-deficit/hyperactivity disorder: A literature review of 2005. Current Psychiatry Reports, 8, 393-397. doi:10.1007/s11920-006-0042-y
[13]	Kaplan, B.J., McNicol, J., Conte, R.A. and Moghadam, H.K. (1989) Dietary replacement in preschool-aged hyperactive boys. Pediatrics, 83, 7-17.
[14]	Carter, C.M., Urbanowicz, M., Hemsley, R., Mantilla, L., Strobel, S., Graham, P.J., et al. (1993) Effects of a few food diet in attention deficit disorder. Archives of Disease in Childhood, 69, 564-568. doi:10.1136/adc.69.5.564
[15]	Ross, M.A. (2000) Could oxidative stress be a factor in neurodevelopmental disorders? Prostaglandins, Leukotrienes and Essential Fatty Acids, 63, 61-63. doi:10.1054/plef.2000.0192
[16]	Chen, J.R., Hsu, S.F., Hsu, C.D., Hwang, L.H. and Yanget, S.C. (2004) Dietary patterns and blood fatty acid composition in children with attention-deficit hyperactivity disorder in Taiwan. Journal of Nutritional Biochemistry, 15, 467-472. doi:10.1016/j.jnutbio.2004.01.008
[17]	Young, G.S., Maharaj, N.J. and Conquer, J.A. (2004) Blood phospholipid fatty acid analysis of adults with and without attention deficit/hyperactivity disorder. Lipids, 39, 117-123.
[18]	Mitchell, E.A., Aman, M.G., Turbott, S.H. and Manku, M. (1987) Clinical characteristics and serum essential fatty acid levels in hyperactive children. Clinical Pediatrics (Phila), 26, 406-411. doi:10.1177/000992288702600805
[19]	Spahis, S., Vanasse, M., Bélanger, S.A., Ghadirian, P., Grenier, E. and Levy, E. (2008) Lipid profile, fatty acid composition and proand anti-oxidant status in pediatric patients with attention-deficit/hyperactivity disorder. Prostaglandins, Leukotrienes and Essential Fatty Acids, 79, 47-53. doi:10.1016/j.plefa.2008.07.005
[20]	Masters, C., Pegg, M. and Crane, D. (1986) On the multiplicity of the enzyme in mammalian liver. Molecular and Cellular Biochemistry, 70, 113-120. doi:10.1007/BF00229426
[21]	Khurana, S., Corbally, M.T., Manning, F., Armenise, T., Kierce, B. and Kilty, C. (2002) Glutathione S-transferase: A potential new marker of intestinal ischemia. Journal of Pediatric Surgery, 37, 1543-1548. doi:10.1053/jpsu.2002.36181
[22]	Awasthi, Y.C., Sharma, R. and Singhal, S.S. (1994) Human glutathione S-transferase. International Journal of Biochemistry, 26, 295-308. doi:10.1016/0020-711X(94)90050-7
[23]	American Psychiatric Association (1994) Diagnostic and statistical manual of mental disorders DSM-IV. 4th Edition, APA Press, Washington DC.
[24]	Beers Jr, R.F. and Sizer, I.W. (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry, 195, 133.
[25]	Habig, W.H., Pabst, M.J. and Jacob, W.B. (1974) Glutathione S-transferase. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249, 7130-7139.
[26]	Koracevic, D., Koracevic, G., Djordjevic, V., Andrejevic, S. and Cosic, V. (2001) Method for the measurement of antioxidant activity in human fluids. Journal of Clinical Pathology, 54, 356-361. doi:10.1136/jcp.54.5.356
[27]	Wayner, D.D.M., Burton, G.W., Ingold, K.U., Barclay, L.R.C. and Locke, S.J. (1987) The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma. Biochimica et Biophysica Acta, 924, 408419. doi:10.1016/0304-4165(87)90155-3
[28]	Woodford, F.P. and Whitehead, T.P. (1998) Is measuring serum antioxidant capacity clinically useful? Annals of Clinical Biochemistry, 35, 48-56.
[29]	Chovanova, Z., Muchova, J., Sivonova, M., Dvorakova, M., Zittnanova, I., Waczulikova, I., et al. (2006) Effect of polyphenolic extract, pycnogenol, on the level of 8oxoguanine in children suffering from attention deficit/ hyperactivity disorder. Free Radical Research, 40, 10031010. doi:10.1080/10715760600824902
[30]	Joshi, K., Lad, S., Kale, M., Patwardhan, B., Mahadik, S.P., Patni, B., et al. (2006) Supplementation with flax oil and vitamin C improves the outcome of Attention Deficit Hyperactivity Disorder (ADHD). Prostaglandins, Leukotrienes and Essential Fatty Acids, 74, 17-21. doi:10.1016/j.plefa.2005.10.001