Influence of Putrescine on Enzymes of Ammonium Assimilation in Maize Seedling

Vineeta Awasthi, Indreshu Kumar Gautam, Rakesh Singh Sengar, Sanjay Kumar Garg

Department of Plant Science, M.J.P. Rohilkhand University, Bareilly, India.
Sardar Vallabh Bhai Patel University of Agriculture and Technology, Meerut, India.

DOI: 10.4236/ajps.2013.42039                

Abstract

The effect of different concentrations of putrescine on biochemical changes in root and shoot of six days old maize seedlings in terms of enzymes of ammonium assimilation were examined. The results revealed that glutamate dehydrogenase (GDH) activity was enhanced at lower concentration of putrescine but at higher concentration, the activity of this enzyme was declined. Glutamine synthetase (GS) activity decreased with increase in concentration of putrescine and it was highest at 1000 μm concentration. Howe ver, glutamate synthase (GOGAT) activity increased with increase in concentration of putrescine upto 100 μm in root and upto 50 μm in shoot and further increase in concentration resulted in decline of enzymatic activity. Protein and total nitrogen content increased upto 10 μm concentration of putrescine and it decreased further with increase in concentration both in root and shoot of maize seedling.

Keywords

Glutamate Dehydrogenase; Glutamine Synthetase; Glutamate Synthase; Maize Seedlings; Putrescine; Zea mays

Conflicts of Interest

The authors declare no conflicts of interest.

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