Fluorescent Voltage Imaging Technique for the Measurement of Molluscan Neural Activities

DOI: 10.4236/ojbiphy.2013.31A007   PDF   HTML   XML   3,503 Downloads   6,552 Views   Citations


The electrophysiological methods using microelectrodes are not appropriate for the simultaneous measurement of neural activities of many neurons. To overcome the difficulty, the fluorescent imaging technique using voltage sensitive dyes can be a powerful technique. The voltage sensitive dyes, however, generally exhibit a relatively small change in their fluorescence intensities, resulting in a low S/N ratio. Additionally, they often exhibit photobleaching and phototoxity. We have therefore improved the fluorescent voltage imaging technique by using a LED as the light source and an electron multiplying (EM)-CCD camera as the fluorescence detector. In this study, we applied our imaging system for the measurement of two kind of molluscan neural activities; one of which is involved in the olfactory processing of the land slug Limax valentianus and the other is involved in the feeding rhythm of the pond snail Lymnaea stagnalis. The system enabled us to measure the neural activities for a long time with a high speed and a high S/N ratio, and the obtained results showed some new physiological findings.

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Y. Hamasaki, M. Hosoi, S. Nakada, T. Shimokawa and M. Saito, "Fluorescent Voltage Imaging Technique for the Measurement of Molluscan Neural Activities," Open Journal of Biophysics, Vol. 3 No. 1A, 2013, pp. 54-58. doi: 10.4236/ojbiphy.2013.31A007.

Conflicts of Interest

The authors declare no conflicts of interest.


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