Study on Characteristics in the Removal Process of Ammonia Nitrogen and Nitrate Nitrogen by an Isolated Heterotrophic Nitrification-Aerobic Denitrification Strain Rhodococcus Sp.


Removal of ammonia nitrogen and nitrate nitrogen by an heterotrophic nitrification-aerobic denitrification strain is an economical and effective method. In this article, a kind of heterotrophic nitrification-aerobic denitrification strain which has aerobic denitrification and heterotrophic nitrification ability was selected, and then was identified as rhodococcus sp. by 16S rRNA sequencing analysis and morphological observation. After that, carbon source utilization and nitrification- denitrification activity of this strain in different C/N, initial nitrogen concentration were studied. In addition, the assimilation and denitrification activities of ammonia and nitrate were also researched under the condition of nitrate and ammonia coexisted in the solution. The results show that the strain can grow in sodium acetate, glucose, sodium succinate and sodium citrate solutions, and it can not survive in sodium oxalate, sucrose and soluble starch solutions. Initial concentration and C/N were important for nitrogen removal rate. This strain can completely remove nitrate/ammonia when nitrate/ammonia concentration was lower than 15 mg l-1/80 mg l-1. the C/N of 10 and of 12 were the optimum C/N ratio in the nitrate and ammonia removal process respectively. pH value rose up sharply in the denitrification process and it increased relatively slowly in the nitrification process, which shows that pH is one of the most important factor inhibiting the denitrification removal process. Nitrite concentration was much higher in denitrification process than in nitrification process. In addition, this strain gave priority to utilizing ammonia as nitrogen source when ammonia and nitrate coexisted in the solution.

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W. Li, "Study on Characteristics in the Removal Process of Ammonia Nitrogen and Nitrate Nitrogen by an Isolated Heterotrophic Nitrification-Aerobic Denitrification Strain Rhodococcus Sp.," Journal of Environmental Protection, Vol. 4 No. 1B, 2013, pp. 74-79. doi: 10.4236/jep.2013.41B014.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] D. D. Foeht, ,W. Versrtaete. “Biochemical ecology of nitrification and dinirtification”. Adv Microbial Ecol, Vol. 1, No. 5, 1997, pp. 135-214.
[2] B. K. Mobarry, M. Wagner, V. Urbain, B. E. Rittmann and D. A. Stahl. “Phylogenetic probes for analyzing abundance and Special organization of nirtrifying bacteria”. APPI Environ Mierobiol., Vol. 62, No. 6, 1996, pp. 2156-2162.
[3] H. S. Joo, M. Hirai, M. Shodal.“Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No. 4”. J Biosci Bioeng, Vol. 100, No. 2, 2005, pp. 184-191.
[4] J. K. Kim, K. J. Park, K. S. Cho. “Aerobic nitrificationdenitrification by heterotrophic Bacillus strains”. Bioresource Technol, Vol. 3, No. 29, 2005, pp. 1897-1906.
[5] P. Mark, D. Dennis. “Focht 15N kinetic analysis N2O production by nirosomonas europaea an examination of nitrifier denitrification”. Applied and Environmental Microbiology, Vol. 49, No. 5, 1985, pp. 1134-1141.
[6] C. Gladys, S. Claudia, V. Idania. “Sulfur-selective desulfurization of dibenzothiophene and diesel oil by newly isolated Rhodococcus sp. strains”. FEMS Microbiology Letters, Vol. 215, No. 1, 2002, pp. 157-161.
[7] N. Takaya, A. B. C. S. Maria, S. Yasushi. “Aerobie denitrifieation bacteria that produce low levels of nitrous oxide”. Appl and Environ Mierobiology, Vol. 69, No. 6, 2003, pp. 3152-3157.
[8] D. J. Richardson, S. J. Ferguson. “The influence of carbon substrate on the activity of the periplasmic nitrate reductase in aerobically grown Thiosphaera pantotropha”. Archives of Microbiology, Vol. 157, 1992, pp. 535-537.
[9] M. Kim, S. Jeong, S. J. Yoon. “Aerobic denitrification of Pseudomonas putida AD-21 at different C/N rations”. J Biosci Bioeng, Vol. 106, No. 5, 2008, pp. 498-502.
[10] H. K. Huang, S. K. Tseng. “Nitrate reduction by Citrobacter diversus under aerobic environment”. Applied Microbiology and Biotechnology,Vol. 55, No. 1, 2001, pp. 90-94.
[11] S. K. Gupta, Monak. “Quantitative estmiation of Thiosphaeta pantotropha from aerobic mixed culture”. Water Research, Vol. 34, No. 15, 2000, pp. 3765-3768.
[12] G. Mevel, D. Prieur. “Heterotrophic nitrification by a thermophilic Bacillus species as influenced by different culture conditions”. Can J Microbiol, Vol. 46, 2000, pp. 465-473.
[13] J. J. Beun, Van, M. C. M. Loosdrecht, J. J. Heijnen. “Aerobic granulation in sequencing batch airlift reactor”. Wat Res, Vol. 36, No. 2, 2002, pp. 702-712.
[14] D. Patureau, N. Bernet, J. P. Delgenes. “Effect of dissolved oxygen and carbon-nitrogen loads on denitrification by an aerobic consortium”. Appl Microbiol Biot, Vol. 54, No. 4, 2000, pp. 535-542.

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