Optimization of Penicillin G Acylase Immobilization Process by Surface Response Methodology Using Central Composite Design

Atyabi Seyed Mohammad, Akbarzadeh Azim, Salimi Mona, Momen Seyed Bahman, Sedigeh Hatami Gigloo, Hossein Nemati, Norouzian Dariush

Physiology and Pharmacology Department, Pasteur Institute of Iran, Tehran, Iran.
Pilot Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran.

DOI: 10.4236/am.2013.41012                

Abstract

Penicillin G acylase was immobilized onto superparamagnetite iron oxide nanoparticles employing response surface methodology through central composite design. Polynomial quadratic model was selected as a model. The value of the determination coefficient (R²) calculated from the quadratic regression model was 0.845, while the value of the adjusted (R²) was 0.74. The regression analysis of the data showed that the quadratic model selected were appropriate thereby enzyme concentration (A), reaction temperature (D), enzyme concentration^* reaction temperature (AD), quadratics enzyme concentration (A²) and reaction temperature (D²) were found to be significant factors in immobilization process of penicillin G acylase.

Keywords

Quadratic Model; Significant; Enzyme Concentration; Temperature of the Adsorption

Conflicts of Interest

The authors declare no conflicts of interest.

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