Aphrodisiac Activity of Aqueous Extract of Phoenix dactylifera Pollen in Male Rats

Ali Abedi, Mohsen Parviz, Seyed Morteza Karimian, Hamid Reza Sadeghipour Rodsari

Physiology Department, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

DOI: 10.4236/asm.2013.31006                

Abstract

Aim of study: Ancient literature alluded to the use of a number of plants/preparations as sex enhancer. One of such botanicals is Phoenix dactylifera in which the pollen grain has been acclaimed to be used as an aphrodisiac. However, the validity has not been scientifically tested. Dopamine is known to facilitate male sexual function. Therefore, the present study was undertaken to evaluate the effect of aqueous extract of Phoenix dactylifera pollen on the sexual behavior of male rats and to measure of serum Estradiol and Testostrone. Also, dopamine transmission in the nucleus accumbence (NAc) was studied in male rats using in vivo microdialysis. Methods and Materials: sixty male rats were randomized into 6 groups (A-F). Group A received 0.2 ml of Normal Saline mixed with Dimethyl Sulphate (DMSO), while groups B-F were injected same volume containing 35 mg/kg, 70 mg/kg, 105 mg/kg, 140 mg/kg and 350 mg/kg of DPP extract, respectively. Sexual behavioral parameters including mounting, intromission and ejaculation frequencies and latencies were recorded in male rats one hour after injection of extract by mating with a receptive female (1:1). The male serum testosterone and estradiol concentrations were also determined. Results: All doses stimulated male sexual behavior. Extract significantly increased mount, ejaculation, intromission frequencies and ejaculation latency in comparison to controlled ones (p < 0.001). Mount and intromission latencies significantly reduced (p < 0.001). Maximum effect was observed in dose 140 mg/kg. This extract was found to enhance Testestrone, Estradiol and the orientation of males toward female ones by increasing mounting and ano-genital investigatory behavior. Conclusions: Data from this study identified that the aqueous extract of Phoenix dactylifera pollen grain enhanced sexual behaviour in male rats. The improved sexual appetitive behaviour in male rats may be attributed, to the alkaloids, saponins, and or flavonoids since these phytochemicals has engorgement, androgen enhancing. Also, our findings support the traditional use of this plant as acclaimed aphrodisiac and for the treatment of pre-ejaculation and impotency.

Keywords

Aphrodisiac Activity; Microdialysis; Dopamine; Testostrone; Estradiol; Male Rat; Phoenix dactylifera Pollen Grain; Pre-Ejaculation; Sexual Behavior

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	J. H. Jung, S. C. Kam, S. M. Choi, S. U. Jae, S. H. Lee, et al., “Sexual Dysfunction in Male Stroke Patients: Correlation between Brain Lesions and Sexual Function,” Urology, Vol. 71, No. 1, 2008, pp. 99-103. doi:10.1016/j.urology.2007.08.045
[2]	M. N. Anil Kumar, N. B. Pai, T. S. Rao and N. Goyal, “Biolgy of Sexual Dysfunction,” Health and Allied Sciences, Vol. 8, No. 1, 2009, pp. 1-7.
[3]	R. L. Meisel and B. D. Sachs, “The Physiology of Male Sexual Behavior,” In: E. Knobil and J. Neill, Physiology of Reproduction, 2nd Edition, Raven Press, New York, 1994, pp. 3-105.
[4]	WHO, “WHO Manual for Standardized Investigation, Diagnosis and Management of the Infertile Male,” Cambridge University Press, Cambridge, 2000, pp. 10-50.
[5]	A. F. G. Cicero, E. Bandieri and R. Arletti, “Lepidium meyenii Improves Sexual Behaviour in Male Rats Independently from Its Action on Spontaneous Locomotor Activity,” Journal of Ethnopharmacology, Vol. 75, No. 2-3, 2001, pp. 225-229. doi:10.1016/S0378-8741(01)00195-7
[6]	A. Adimoelja, “Phytochemicals and the Breakthrough of Traditional Herbs in the Management of Sexual Dysfunctions,” International Journal of Andrology, Vol. 23, No. 2, 2000, pp. 82-84. doi:10.1046/j.1365-2605.2000.00020.x
[7]	A. J. Krentz and C. J. Bailey, “Oral Antidiabetic Agents: Current Role in Type 2 Diabetes Mellitus,” Drugs, Vol. 65, No. 3, 2005, pp. 385-411. doi:10.2165/00003495-200565030-00005
[8]	Y. K. Gupta and S. Briyal, “Animal Models of Cerebral Ischemia for Evaluation of Drugs,” Indian Journal of Physiology and Pharmacology, Vol. 48, No. 4, 2004, pp. 379-394.
[9]	M. W. Islam, M. Tariq, A. M. Ageel, M. S. Al-said and A. M. Al-Yaya, “Effect of Salvia hematodes on Sexual Behavior of Male Rats,” Journal of Ethnopharmacology, Vol. 33, 1991, pp. 67-72. doi:10.1016/0378-8741(91)90163-8
[10]	S. Bahmanpour, T. Talaei, Z. Vojdani, M. R. Panjehshahin, A. Poostpasand, S. Zareei and M. Ghaeminia, “Effect of Phoenix dactylifera Pollen on Sperm Parameters and Reproductive System of Adult Male Rats,” International Journal of Molecular Sciences, Vol. 31, No. 4, 2006, pp. 208-212.
[11]	F. Biglari, A. F. M. AlKarkhi and M. E. Azhar, “Antioxidant Activity and Phenolic Content of Various Date Palm (Phoenix dactylifera) Fruits from Iran,” Food Chemistry, Vol. 107, No. 4, 2008, pp. 1636-1641. doi:10.1016/j.foodchem.2007.10.033
[12]	A. A. Al-Qarawi, H. M. Mousa, B. E. H. Ali, H. Abdel-Rahman and S. A. El-Mougy, “Protective Effect of Extracts from Dates (Phoenix dactylifera) on Carbon Tetrachloride-Induced Hepatotoxicity in Rats,” The Journal of Applied Research in Veterinary Medicine, Vol. 2, No. 3, 2004, pp. 176-180.
[13]	A. Agmo, “Male Rat Sexual Behaviour,” Brain Research Protocols, Vol. 1, No. 2, 1997, pp. 203-209. doi:10.1016/S1385-299X(96)00036-0
[14]	R. L. Meisel, J. K. O’Hanlon and B. D. Sachs, “Differential Maintenance of Penile Responses and Copulatory Behaviour by Gonadal Hormones in Castrated Male Rats,” Hormones and Behavior, Vol. 18, No. 1, 1984, pp. 54-56. doi:10.1016/0018-506X(84)90050-3
[15]	M. A. Ageel, M. W. Islam, O. T. Ginawi and M. A. Al-Yahya, “Evaluation of the Aphrodisiac Activity of Litsea chinenesis and Orchis masculata Extract in Rats,” Phytotherapy Research, Vol. 8, No. 2, 1994, pp. 103-105. doi:10.1002/ptr.2650080211
[16]	R. C. Schiavi and R. T. Segraves, “The Biology of Sexual Function,” Annals of Clinical Psychiatry, Vol. 7, No. 4, 1995, pp. 189-201.
[17]	H. J. Kim, D. S. Woo and J. J. Lee Kim, “The Relaxation Effects of Ginseng Saponin in Rabbit Corporal Smooth Muscle, Is It a Nitric Oxide Donor?” British Journal of Urology, Vol. 82, No. 5, 1998, pp. 744-748. doi:10.1046/j.1464-410X.1998.00811.x
[18]	S. A. Padashetty and S. H. Mishra, “Aphrodisiac Studies of Tricholepis glaberrima with Supportive Action from Antioxidant Enzymes,” Pharmaceutical Biology, Vol. 45, No. 7, 2007, pp. 580-586. doi:10.1080/13880200701501326
[19]	A. Morales, D. H. C. Surridge, P. G. Marshall and J. Fenemote, “Nonhormonal Pharmacology Treatment of Organic Impotence,” Journal of Urology, Vol. 128, 1982, pp. 45-47.
[20]	F. Giuliano, O. Rampin, A. Schiar, A. Jardin and J. P. Rousseau, “Autonomic Control of Penile Erection: Modulation by Testosterone in the Rat,” Journal of Neuroendocrinology, Vol. 9, 1993, pp. 141-150.
[21]	T. M. Mills, V. S. Stopper and V. T. Wiedmeier, “Effects of Castration and Androgen Replacement on the Hemodynamics of Penile Erection in the Rat,” Biology of Reproduction, Vol. 51, No. 2, 1994, pp. 234-238. doi:10.1095/biolreprod51.2.234
[22]	M. Thakur and V. K. Dixit, “Aphrodisiac Activity of Dactylorhiza hatagirea (D. Don) Soo in Male Albino Rats,” Evidence-Based Complementary and Alternative Medicine, Vol. 4, No. 1, 2007, pp. 29-31. doi:10.1093/ecam/nem111
[23]	M. D. Majewska, F. L. Bellino, R. A. Davies, P. J. Hornsby, D. H. Lavrin and J. E. Nestler, “Neuronal Activities of Dehydroepiandrosterone in Dehydroepiandrosterone (DHEA) and Aging,” The New York Academy of Sciences, Vol. 774, 1995, pp. 111-120 . doi:10.1111/j.1749-6632.1995.tb17375.x
[24]	M. T. Yakubu, M. A. Akanji, A. T. Oladiji and A. A. Adesokan, “Androgenic Potentials of Aqueous Extract of Massularia acuminata (G. Don) Bullock ex Hoyl. Stem in Male Wistar Rats,” Journal of Ethnopharmacology, Vol. 118, No. 3, 2008, pp. 508-513. doi:10.1016/j.jep.2008.05.020
[25]	P. K. Suresh Kumar, A. Subramoniam and P. Pushpangadan, “Aphrodisiac Activity of Vanda tessellata (Roxb.) Hook. ex Don Extract in Male Mice,” Indian Journal of Pharmacology, Vol. 32, No. 5, 2000, pp. 300-304.
[26]	M. T. Yakubu, M. A. Akanji and A. T. Oladiji, “Aphrodisiac Potentials of the Aqueous Extract of Fadogia agrestis (Schweinf ex Hiern) Stem in Male Albino Rats,” Asian Journal of Andrology, Vol. 7, No. 4, 2005, pp. 399-404. doi:10.1111/j.1745-7262.2005.00052.x
[27]	G. H. Mahran, S. M. Abdul-Wahab and A. M. Attia, “A Phytochemical Study of Date Palm Pollen,” Planta Medica, Vol. 29, No. 2, 1976, pp. 171-175. doi:10.1055/s-0028-1097648
[28]	E. Amine, O. Awad, El-Samad and M. N. Iskander, “Pharmacological Studies on Pollen Grains of Dates (Pheonix dactylefera),” Phytochemistry, Vol. 8, 1996, pp. 295-298.
[29]	M. R. Zarrindast, K. Nojoomi, M. Sharifzadeh and A. Mokri, “Niric Oxide Agents and Apomorphine-Induced Rat Behaviors,” Pharmacology, Vol. 71, No. 4, 2003, pp. 169-173 doi:10.1159/000078082
[30]	S. K. Putnam, J. Du and E. M. Hull, “Testosterone Restoration of Copulation and Medial Preoptic Dopamine Release in Castrated Male Rats: 2-, 5-, and 10-Day Treatments,” Hormones and Behavior, Vol. 39, No. 3, 2001, pp. 216-224. doi:10.1006/hbeh.2001.1648
[31]	C. L. Ballard and R. I. Wood, “Partner Preference in Male Hamsters: Steroids, Sexual Experience and Chemosensory Cues,” Physiology and Behavior, Vol. 91, No. 1, 2007, pp. 1-8. doi:10.1016/j.physbeh.2007.01.005
[32]	K. Gauthaman and P. G. Adaikan, “The Hormonal Effects of Tribulus terrestris and Its Role in the Management of Male Erectile Dysfunction—An Evaluation Using Primates, Rabbit and Rat,” Phytomedicine, Vol. 15, No. 1, 2008, pp. 44-54. doi:10.1016/j.phymed.2007.11.011
[33]	P. Sandroni, “Aphrodisiacs Past and Present, a Historical Review,” Clinical Autonomic Research, Vol. 11, No. 5, 2001, pp. 303-307. doi:10.1007/BF02332975
[34]	A. Resho and Al-Shagrawi, “Enzyme Activities, Lipid Fractions, and Fatty Acids Composition in Male Rats Fed Palm Pollen Grains (Phoenix dactylifera),” Research Bulletin, Vol. 79, 1998, pp. 5-18.