Creatine supplementation in exercised rats: Effects on the aerobic capacity

Michel Barbosa de Araújo, Roberto C. Vieira Junior, Leandro P. Moura, Marcelo Costa Junior, Rodrigo A. Dalia, Amanda Christine da Silva Sponton, Carla Ribeiro, Maria Alice R. Mello

Biosciences Institute, Department of Physical Education, Universidade Estadual Paulista Júlio de Mesquita Filho, Rio Claro, Brazil.
Biosciences Institute, Department of Physical Education, Universidade Estadual Paulista Júlio de Mesquita Filho, Rio Claro, Brazil;.
Faculty of Nutrition, Universidade Federal de Mato Grosso, Cuiabá, Brazil.

DOI: 10.4236/ojas.2013.31003                

Abstract

This study aimed to analyze the possible metabolic disturbances caused by creatine supplementation on aerobic capacity of rats, inferred by the maximal lactate steady state. Forty male Wistar rats (90 days old) were distributed into two groups for eight weeks: trained group (T): rats that were submitted to a training protocol, and supplemented-trained group (TCr): rats that were submitted to a training protocol and received balanced diet supplemented with 2% creatine. The blood lactate concentrations equivalent to maximal lactate steady state during treadmill running were analyzed at the beginning and also at the end of the experiment. At the end of the experiment were done comparing the test results MLSS between the two groups. At the beginning of the experiment, prior to groups division, the majority of animals obtained MLSS at a speed of26 m/min, blood lactate concentration of 3.79 ± 0.76 mmol/L. At the end of the experiment, most of trained rats in T presented MLSS at the speed of28 m/min, blood lactate concentration of 3.37 ± 0.68 mmol/L. Most TCr had MLSS at the speed of28 m/min, blood lactate concentration of 3.52 ± 0.69 mmol/L. We conclude that creatine supplementation was not the cause of the improvement in the aerobic capacity of rats in the tread-mill exercise.

Keywords

Diet; Lactate; Maximal Lactate Steady State; Treadmill Running

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Stroud, M.A., Holliman, D., Bell, D., Green, A.L., Macdonald, I. and Greenhaff, P.L. (1994) Effect of oral creatine supplementation on respiratory gas exchange and blood lactate accumulation during steady-state incremental tread-mill exercise and recovery in man. Clinical Science, 87, 707-710.
[2]	Williams, M.H., Kreider, R. and Branch, J.D. (2000) Creatina. Ed Manole, S?o Paulo.
[3]	Engelhardt, M., Neumann, G., Berbalk, A. and Reuter, I. (1998) Creatine supplementation in endurance sports. Medicine Science Sports Exercise, 30, 1123-1129. doi:10.1097/00005768-199807000-00016
[4]	Souza Junior, T.P., Dubas, J.P., Pereira, B. and Oliveira, P.R. (2007) Suplementa??o de creatina e treinamento de for?a: Altera??es na resultante da for?a máxima dinamica e variáveis antropométricas em universitários submetidos a oito semanas de treinamento de for?a (hipertrofia). Revista Brasileira de Medicina do Esporte, 13, 303-309.
[5]	Gama, M.S. (2011) Efeitos da creatina sobre desempenho aeróbio: Uma revis?o sistemática. Revista Brasileira de Nutri??o Esportiva, 5, 182-190.
[6]	Pereira Junior, M., Moraes, A.J.P., Ornellas, F.H., Gon- ?alves, M.A., Liberali, R. and Navarro, F. (2012) Efi- ciência da suplementa??o de creatina no desempenho físico humano. Revista Brasileira de Prescri??o e Fisiologia do Exercício, 6, 90-97.
[7]	Araújo, M.B. and Mello, M.A.R. (2009) Exercício, estresse oxidativo e suplementa??o com creatina. Revista Bra- sileira de Nutri??o Esportiva, 3, 264-272.
[8]	Greenhaff, P.L. (1997) The nutritional biochemistry of creatine. Journal Nutrition Biochemistry, 11, 610-618. doi:10.1016/S0955-2863(97)00116-2
[9]	Manchado, F.B., Gobatto, C.A., Contarteze, R.V.L., Pa- poti, M. and Mello, M.A.R. (2005) Maximal lactate steady in running rats. Journal Exercise Physiology, 8, 29-35.
[10]	Pringle, J.S.M. and Jones, A.M. (2002) Maximal lactate steady state, critical power and EMG during cycling. European Journal Applied Physiology, 88, 214-226. doi:10.1007/s00421-002-0703-4
[11]	Billat, V.L., Siverent, P., Py, G., Korallsztein, J.P. and Mercier, J. (2003) The concept of maximal lactate steady state: A bridge between biochemistry, physiology and sport science. Sports Medicine, 33, 407-426. doi:10.2165/00007256-200333060-00003
[12]	Manchado-Gobatto, F.B. (2007) Protocolos invasivos e n?o invasivos para avalia??o aeróbia e anaeróbia de ratos wistar. Tese de Doutorado, Pós-Gradua??o em Ciências da Motricidade Humana, UNESP, Rio Claro.
[13]	Jones, A.M. and Carter, H. (2000) The effect of endurance training on parameters of aerobic fitness. Sports Medicine, 29, 373-376. doi:10.2165/00007256-200029060-00001
[14]	Baldari, C. and Guidetti, L. (2000) A simple method for individual anaerobic threshold as a predictor of maximal lactate steady state. Medicine Science Sports Exercise, 32, 1798-1802. doi:10.1097/00005768-200010000-00022
[15]	Almarwaey, O.A., Jones, A.M. and Tolfrey, K. (2004) Maximal lactate steady state in trained adolescent runners. Journal Sports Science, 22, 215-225. doi:10.1080/02640410310001641520
[16]	Gobatto, C.A., Mello, M.A.R., Sibuya, C.Y., Azevedo, J.R.M., Santos, L.A. and Kokubun, E. (2001) Maximal lactate steady state in rats submitted to swimming exercise. Comparative Biochemistry Physiology, 130, 21-27. doi:10.1016/S1095-6433(01)00362-2
[17]	Araújo, M.B., Moura, L.P., Ribeiro, C., Dalia, R.A., Vol- tarelli, F.A. and Mello, M.A.R. (2011) Oxidative stress in the liver of exercised rats supplemented with creatine. International Journal of Nutrition and Metabolism, 3, 58- 64.
[18]	Reeves, P.G., Nielsen, F.H. and Fahey, G.C. (1993) AIN- 93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc writing com- mittee on the reformulation of the AIN-76A rodent diet. Journal Nutrition, 123, 1939-1951.
[19]	Deminice, R., Portari, G.V., Vannucchi, H. and Jord?o, A.A. (2009) Effects of creatine supplementation on ho- mocysteine levels and lipid peroxidation in rats. Brazilian Journal Nutrition, 102, 110-116. doi:10.1017/S0007114508162985
[20]	Hultman, E., Soderlund, K., Timmons, J., Cederblad, G. and Greenhaff, P. (1996) Musclecreatine loading in man. Journal Applied Physiology, 81, 232-237.
[21]	Vandenberghe, K., Goris, M., Van Hecke, P., Van Leem-putte, M., Vangerven, L. and Hespel, P. (1997) Long-term creatine intake is beneficial to muscle performance during resistance training. Journal Applied Physiology, 83, 2055-2063.
[22]	Engels, R.C. and Jones, J.B. (1978) Causes and elimination of erratic blanc in enzymatic metabolic assays involving the use of NAD in alkaline hydrazine buffers: Improved conditions for assay of L-glutamate, L-lactate and other metabolites. Analytical Biochemistry, 88, 475- 484. doi:10.1016/0003-2697(78)90447-5
[23]	Bemben, M.G. and Lamont, H.S. (2005) Creatine sup- plementation and exercise performance: Recent findings. Sports Medicine, 35, 107-125. doi:10.2165/00007256-200535020-00002
[24]	Souza, R.A., Santos, R.M., Osório, R.A., Cogo, J.C., Prianti Júnior, A.C.G., Martins, R.A.B.L. and Ribeiro, W. (2006) Influência da suplementa??o aguda e cr?nica de creatina sobre as concentra??es sanguíneas de glicose e lactato de ratos Wistar. Revista Brasileira de Medicina do Esporte, 12, 361-365. doi:10.1590/S1517-86922006000600012
[25]	Contontarteze, R.V.L., Manchado, F.B., Gobatto, C.A. and Mello, M.A.R. (2007) Stress biomarkers in rats submitted to swimming and treadmil running exercises. Compara- tive Biochemistry Physi-ology, A, 1-8.
[26]	Pillis, W., Zarzeczny, R., Langfort, J., Ka-ciuba-Uscilko, H., Nazar, K. and Wojtyna, J. (1993) Anaerobic threshold in rats. Comparative Biochemistry Physiology, 106, 285-289. doi:10.1016/0300-9629(93)90513-4
[27]	Langfort, J., Zarzeczny, R., Pillis, W., Kaciuba-Uscilko, H., Nazar, K. and Porta, S. (1996) Effect of sustained hyperadrenalinemia on exercise performance and lactate threshold in rats. Comparative Biochemistry Physiology, 14, 51-55. doi:10.1016/0300-9629(95)02087-X
[28]	Araújo, M.B., Voltarelli, F.A., Manchado-Gobatto, F.B., Moura, L.P. and Mello, M.A.R. (2010) Treinamento em diferentes intensidades e biomarcadores de estresse oxidativo e do metabolismo glicídico musculoesquelético de ratos. Revistade Educa??o Física/UEM, 21, 695-707.
[29]	Kreider, R.B. (2003) Species-specific responses to creatine supplementation. American Journal of Physiology—Regulatory, Integrative and Comparative Physiology, 285, R725-R726.
[30]	Freire, T.O., Gulano, B., Leme, M.D., Polacow, V.O. and Lancha Júnior, A.H. (2008) Capta??o de glicose em ratos submetidos ao exercício físico. Revista Brasileira de Medicina do Esporte, 14, 431-435. doi:10.1590/S1517-86922008000500006
[31]	Casey, A. and Greenhaff, P.L. (2000) Does dietary creatine supplementation play a role in skeletal muscle metabolism and performance? American Journal Clinical Nutrition, 72, 607S-617S.
[32]	Wasserman, K. (1986) The anaerobic threshold: Definition, physiological significance and identification. Advances in Cardiology, 35, 1-23.
[33]	Mcardle, W.D., Katch, F.I. and Katch, V.L. (2008) Fisiologia do exercício: Energia, nutri??o e desempenho humano. Editora Guanabara Koogan S.A., Rio de Janeiro.
[34]	Gobatto, C.A., Kokubun, E., Sibuya, C.Y. and Mello, M.A.R. (1991) Efeitos da desnutri??o proteica-calórico e do treinamento físico na produ??o de acido lático em ratos machos adultos apos testes de cargas progressivas. Ciência e Cultura, 43, 725-736.
[35]	Araújo, M.B., Manchado-Gobatto, F.B., Voltarelli, F.A., Ribeiro, C., Mota, C.S.A., Gobatto, C.A. and Mello, M.A.R. (2009) Efeitos do treinamento de corrida em diferentes intensidades sobre a capacidade aeróbia e produ??o de lactato pelo musculo de ratos wistar. Revista Brasileira de Medicinado Esporte, 15, 365-369. doi:10.1590/S1517-86922009000600009
[36]	Voltarelli, F.A., Mello, M.A.R. and Gobatto, C.A. (2004) Limiar anaeróbio determinado pelo teste do lactato míni- mo em ratos: Efeito dos estoques de glicogênio muscular e do treinamento Físico. Revista Portuguesa de Ciência Desportiva, 4, 16-25.