Conjugation of the Recombinant Third Domain of Human Alpha-fetoprotein with Doxorubicin Using Poly(amidoamine) Dendrimers and Study of Its Cytotoxic Activity

DOI: 10.4236/eng.2012.410B021   PDF   HTML     2,889 Downloads   3,984 Views   Citations


The recombinant third domain of alpha-fetoprotein (AFP3D) is one of the promising vectors for targeted delivery of anticancer drugs to tumor cells. AFP3D-based conjugates with various anti-tumor agents have shown specific anti-tumor activity. The content of anti-tumor drugs in the preparation could be increased by including dendrimers - highly branched nanoparticles - into a targeted delivery system. In this study, we synthesized the AFP3D conjugate with doxorubicin-loaded poly(amidoamine) (PAMAM) dendrimer nanoparticles and investigated its cytotoxic activity. 

Share and Cite:

S. Kisil, V. Chernikov, M. Danilevskiy, L. Savvateeva and N. Gorokhovets, "Conjugation of the Recombinant Third Domain of Human Alpha-fetoprotein with Doxorubicin Using Poly(amidoamine) Dendrimers and Study of Its Cytotoxic Activity," Engineering, Vol. 4 No. 10B, 2012, pp. 80-83. doi: 10.4236/eng.2012.410B021.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] B.F.Crandall, Alpha-fetoprotein, CRC Rev. Clin. Lab. Sci., vol. 15, pp. 127-185, 1981.
[2] R. Chaturvedi, V. Agarkar, G. L. Sharma, P.U. Sarma, Purification of alpha-fetoprotein from human cord blood, Prep. Biochem. Biotechnol., pp. 293-303, April 1998.
[3] H. F. Deutsch, Chemisry and Biology of α-fetoprotein, Adv. Cancer Res., vol.56, 1991, pp. 253-312.
[4] S. Nishi, H. Hirai, Purification of human, dog and rabbit α-fetoprotein by immunoadsorbents of Sepharose coupled with anti-human α-fetoprotein, Biochim. Biophys. Acta, vol. 278, pp. 293-298, 1972.
[5] R. Boismenu, D. Semeniuk, R. A. Murgita, Purification and characterization of human and mouse recombinant alpha-fetoproteins expressed in Escherichia coli, Protein Expression and Purif., vol. 10, pp.10-26, 1997.
[6] Posypanova G. A., Gorokhovets N. V., Makarov V. A., Savvateeva L. V., Kireeva N. N., Severin S. E., Severin E. S., Recombinant alpha-fetoprotein C-terminal fragment: the new recombinant vector for targeted delivery. J Drug Target., vol.16, pp. 321-328, May 2008.
[7] Savvateeva L. V., Gorokhovets N. V., Makarov V. A., Posypanova G. A., Severin S. E., Recombinant third domain of human alpha-fetoprotein: selectivity and antitumor activity of conjugate with Taxol. Abstracts of the XXXIII Meeting of the International Society for Oncodevelopmental Biology and Medicine (ISOBM), Rhodes, Greece, p. 84., September 2005.

comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.