Rapid Determination of Sexually Transmitted Infections by Real-time Polymerase Chain Reaction Using Microchip Analyzer

O. Suvorova; A. Perchik; M. Slyadnev; O. Suvorova; A. Perchik; M. Slyadnev; D. Navolotskii; N. Mushnikov

doi:10.4236/eng.2012.410B001

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Engineering > Vol.4 No.10B, October 2012

Rapid Determination of Sexually Transmitted Infections by Real-time Polymerase Chain Reaction Using Microchip Analyzer ()

O. Suvorova, A. Perchik, M. Slyadnev, O. Suvorova, A. Perchik, M. Slyadnev, D. Navolotskii, N. Mushnikov

Department of chemistry, Saint Petersburg State University, Saint Petersburg, Russia.
Department of chemistry, Saint Petersburg State University, Saint Petersburg, Russia Saint Petersburg, Russia.
Lumex Ltd. Saint Petersburg, Russia.
Lumex Ltd., Saint Petersburg, Russia.

DOI: 10.4236/eng.2012.410B001 PDF HTML 2,941 Downloads 3,988 Views Citations

Abstract

Development of non expensive and time-saving techniques based on the polymerase chain reaction (PCR) is of great importance for modern diagnostics. We considered a new approach for PCR determination of a variety of sexually transmitted infections using microchip analyzer “AriaDNA”, which had been tested using clinical samples in several medical institutions of St. Petersburg (Russia). The use of microchips containing lyophilized PCR reagents allows reducing significantly time of analysis and the number of manipulations thus preventing possible sample contamination.

Keywords

Polymerase Chain Reaction; Sexually Transmitted Infections; Microchip

Share and Cite:

O. Suvorova, A. Perchik, M. Slyadnev, O. Suvorova, A. Perchik, M. Slyadnev, D. Navolotskii and N. Mushnikov, "Rapid Determination of Sexually Transmitted Infections by Real-time Polymerase Chain Reaction Using Microchip Analyzer," Engineering, Vol. 4 No. 10B, 2012, pp. 1-3. doi: 10.4236/eng.2012.410B001.

Conflicts of Interest

The authors declare no conflicts of interest.

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