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Modulation of Sodium-Dependent Transporters Expression in Normal Human Keratinocytes by a Sodium Rich Isotonic Thermal Water

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DOI: 10.4236/jcdsa.2012.24048    2,900 Downloads   4,434 Views   Citations

ABSTRACT

Background/Aim: In order to show that water can participate to the skin defense in front of different stress, we investigated the effect of an isotonic thermal water notably rich in Sodium (i.e. the Uriage thermal water) on 1) The taurine transporter (TauT) expression in human normal keratinocytes irradiated or not by UVB; and 2) the Sodium-dependent vitamin C transporter 1 (SVCT1) expression in human normal keratinocytes issued from two “young” and two “aged” subjects, irradiated or not by UVB. Methods and Results: Using sensible and specific TAUT and SVCT1 ELISA assays developed in house, we provide 1) the unambiguous demonstration that the Uriage thermal water is able to help the epidermis to maintain its taurine content under UVB irradiation; 2) the first example of an altered SVCT1 expression in “aged” keratinocytes and of a significant positive effect of the Uriage thermal water on this altered SVCT1 production; and 3) arguments showing that Uriage thermal water is also able to participate to the regulation of the SVCT1 production in UVB-irradiated keratinocytes. Conclusion: Taking together, these results suggest that the Uriage thermal water could act to efficiently protect the skin from dehydration through its effect on TauT and SVCT1 expression, and furthermore, to allow a more efficient taurine and ascorbic acid supplying to the epidermis in order to protect him from other aggressions such as oxidant stress for example.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

C. Verdy, J. Branka and L. Lefeuvre, "Modulation of Sodium-Dependent Transporters Expression in Normal Human Keratinocytes by a Sodium Rich Isotonic Thermal Water," Journal of Cosmetics, Dermatological Sciences and Applications, Vol. 2 No. 4, 2012, pp. 254-262. doi: 10.4236/jcdsa.2012.24048.

References

[1] H. Morrison, “Photochemistry and Photobiology of Urocanic Acid,” Photochemistry and Photobiology, Vol. 43, No. 6, 1986, pp. 663-665.
[2] Y. Miyamura, et al., “Regulation of Human Skin Pigmentation and Responses to Ultraviolet Radiation,” Pigment Cell Research, Vol. 20, No. 1, 2007, pp. 2-13. doi:10.1111/j.1600-0749.2006.00358.x
[3] B. E. Johnson, “The Influence of Radiation on the Skin and the Basis of Protection,” International Journal of Cosmetic Science, Vol. 5, No. 4, 1983, pp. 131-139. doi:10.1111/j.1467-2494.1983.tb00334.x
[4] R. Kohen, “Skin Antioxidants: Their Role in Aging and in Oxidative Stress-New Approaches for Their Evaluation,” Biomedicine & Pharmacotherapy, Vol. 53, No. 4, 1999, pp. 181-192. doi:10.1016/S0753-3322(99)80087-0
[5] C. Cao, et al., “All-Trans Retinoic Acid Attenuates Ultraviolet Radiation-Induced Down-Regulation of Aquaporin-3 and Water Permeability in Human Keratinocytes,” Journal of Cellular Physiology, Vol. 215, No. 2, 2008, pp. 506-516. doi:10.1002/jcp.21336
[6] G. Janeke, et al., “Role of Taurine Accumulation in Keratinocyte Hydration,” Journal of Investigative Dermatology, Vol. 12, No. 2, 2003, pp. 354-361. doi:10.1046/j.1523-1747.2003.12366.x
[7] H. Steiling, et al., “Sodium-Dependent Vitamin C Transporter Isoforms in Skin: Distribution, Kinetics, and Effect of UVB-Induced Oxidative Stress,” Free Radical Biology and Medicine, Vol. 43, No. 5, 2007, pp. 752-762. doi:10.1016/j.freeradbiomed.2007.05.001
[8] F. Grafe, W. Wohlrab, R. H. Neubert and M. Brandsch, “Functional Characterization of Sodium and ChlorideDependent Taurine Transport in Human Keratinocytes,” European Journal of Pharmaceutics and Biopharmaceutics, Vol. 57, No. 2, 2004, pp. 337-341. doi:10.1016/j.ejpb.2003.10.010
[9] X. Wu, et al., “Zinc-Induced Sodium-Dependent Vitamin C Transporter 2 Expression: Potent Roles in Osteoblast Differentiation,” Archives of Biochemistry and Biophysics, Vol. 420, No. 1, 2003, pp. 114-120. doi:10.1016/j.abb.2003.09.013
[10] H. Qiao and J. M. May, “Development of Ascorbate Transporters in Brain Cortical Capillary Endothelial Cells in Culture,” Brain Research, Vol. 1208, 2008, pp. 79-86. doi:10.1016/j.brainres.2008.02.102
[11] K. Erikson and M. Aschner, “Manganese Causes Differential Regulation of Glutamate Transporter (GLAST) Taurine Transporter and Metallothionein in Cultured Rat Astrocytes,” Neurotoxicology, Vol. 23, No. 4-5, 2003, pp. 595-602. doi:10.1016/S0161-813X(02)00012-8
[12] G. H. Jin, Y. Liu, S. Z. Jin, X. D. Liu and S. Z. Liu, “UVB Induced Oxidative Stress in Human Keratinocytes and Protective Effect of Antioxidant Agents,” Radiation and Environmental Biophysics, Vol. 46, No. 1, 2007, pp. 61-68. doi:10.1007/s00411-007-0096-1
[13] Y. Uchida, M. Behne, D. Quiec, P. M. Elias and W. M. Holleran, “Vitamin C Stimulates Sphingolipid Production and Markers of Barrier Formation in Submerged Human Keratinocyte Cultures,” Journal of Investigative Dermatology, Vol. 117, No. 5, 2001, pp. 1307-1313. doi:10.1046/j.0022-202x.2001.01555.x
[14] W. E. Parish, J. Read and S. E. Paterson, “Changes in Basal Cell Mitosis and Transepidermal Water Loss in Skin Cultures Treated with Vitamins C and E,” Experimental Dermatology, Vol. 14, No. 9, 2005, pp. 684-691. doi:10.1111/j.0906-6705.2005.00340.x
[15] M. Bradford, “A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding,” Analytical Biochemistry, Vol. 72, No. 1-2, 1976, pp. 248-254. doi:10.1016/0003-2697(76)90527-3
[16] N. Rockel et al., “The Osmolyte Taurine Protects against Ultraviolet B Radiation-Induced Immunosuppression,” Journal of Immunology, Vol. 179, No. 6, 2007, pp. 3604-3612.
[17] U. Warskulat, S. Brookmann, A. Reinen and D. H?ussinger, “Ultraviolet B Radiation Induces Cell Shrinkage and Increases Osmolyte Transporter mRNA Expression and Osmolyte Uptake in HaCaT Keratinocytes,” Biological Chemistry, Vol. 388, No. 12, 2007, pp. 1345-1352. doi:10.1515/BC.2007.140
[18] M. L. Tappaz, “Taurine Biosynthetic Enzymes and Taurine Transporter: Molecular Identification and Regulations,” Neurochemical Research, Vol. 29, No. 1, 2004, pp. 83-96. doi:10.1023/B:NERE.0000010436.44223.f8
[19] X. Han, A. B. Patters, D. P. Jones, I. Zelikovic and R. W. Chesney, “The Taurine Transporter: Mechanisms of Regulation,” Acta Physiologica, Vol. 187, No. 1-2, 2006, pp. 61-73. doi:10.1111/j.1748-1716.2006.01573.x
[20] X. Wu et al., “Zinc-Induced Sodium-Dependent Vitamin C Transporter 2 Expression: Potent Roles in Osteoblast Differentiation,” Archives of Biochemistry and Biophysics, Vol. 420, No. 1, 2003, pp. 114-120. doi:10.1016/j.abb.2003.09.013
[21] N. Leveque, S. Robin, S. Makki, P. Muret, A. Rougier and P. Humbert, “Iron and Ascorbic Acid Concentrations in Human Dermis with Regard to Age and Body Sites,” Gerontology, Vol. 49, No. 2, 2003, pp. 117-122. doi:10.1159/000067951
[22] I. Savini, A. Rossi, C. Pierro, L. Avigliano and M. V. Catani, “SVCT1 and SVCT2: Key Proteins for Vitamin C Uptake,” Amino Acids, Vol. 34, No. 3, 2008, pp. 347-355. doi:10.1007/s00726-007-0555-7

  
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