Mass Spectrometric Identification of Propionibacterium Isolates Requires Database Enrichment

DOI: 10.4236/aim.2012.24063   PDF   HTML     4,543 Downloads   7,406 Views   Citations


Propionibacterium species are mostly environmental bacteria, some being commensal of mammals including humans, and sometimes pathogenic. These bacteria are poorly identified using routine laboratory methods. Recently, Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) has emerged as a rapid and efficient method to identify bacterial species. We evaluated the use of MALDI-TOF-MS for identification of all validated Propionibacterium species. Only four of the 15 tested reference strains (26.7%) were correctly identified at the species level, and P. acnes, the most common human pathogenic species was not identified. When applying MALDI-TOF-MS to 48 P. acnes strains, only 18.7% were correctly identified, suggesting an intraspecific variability of proteic profiles among Propionibacterium strains. However, by enriching the Bruker database with spectra from five of these strains and re-testing the other 43 strains against this new database, 93.0% were correctly identified. Our study demonstrates that MALDI-TOF-MS may be used for the identification of Propionibacterium isolates but requires a database enrichment in spectra from additional isolates.

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S. Edouard, C. Couderc, D. Raoult and P. Fournier, "Mass Spectrometric Identification of Propionibacterium Isolates Requires Database Enrichment," Advances in Microbiology, Vol. 2 No. 4, 2012, pp. 497-504. doi: 10.4236/aim.2012.24063.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] I. Brook and E. H. Frazier, “Infections Caused by Propionibacterium Species,” Reviews of Infectious Diseases, Vol. 13, No. 5, 1991, pp. 819-822. doi:10.1093/clinids/13.5.819
[2] C. Dessinioti and A. D. Katsambas, “The Role of Propionibacterium acnes in Acne Pathogenesis: Facts and Controversies,” Clinics in Dermatology, Vol. 28, No. 1, 2010, pp. 2-7. doi:10.1016/j.clindermatol.2009.03.011
[3] P. Y. Levy, F. Fenollar, A. Stein, F. Borrione, E. Cohen, B. Lebail and D. Raoult, “Propionibacterium acnes Post-operative Shoulder Arthritis: An Emerging Clinical Entity,” Clinical Infectious Diseases, Vol. 46, No. 12, 2008, pp. 1884-1886. doi:10.1086/588477
[4] A. L. Perry and P. A. Lambert, “Propionibacterium acnes,” Letters in Applied Microbiology, Vol. 42, No. 3, 2006, pp. 185-188. doi:10.1111/j.1472-765X.2006.01866.x
[5] T. Schaeverbeke, L. Lequen, B. B. De, L. Labbe, C. M. Bebear, Y. Morrier, B. Bannwarth, C. Bebear and J. Dehais, “Propionibacterium acnes Isolated from Synovial Tissue and Fluid in a Patient with Oligoarthritis Associated with Acne and Pustulosis,” Arthritis & Rheumatism, Vol. 41, No. 10, 1998, pp. 1889-1893. doi:10.1002/1529-0131(199810)41:10<1889::AID-ART23>3.0.CO;2-F
[6] S. Panagea, J. E. Corkill, M. J. Hershman and C. M. Parry, “Breast Abscess Caused by Propionibacterium avidum Following Breast Reduction Surgery: Case Report and Review of the Literature,” Journal of Infection, Vol. 51, No. 5, 2005, pp. e253-e255. doi:10.1016/j.jinf.2005.04.005
[7] C. Branger, B. Bruneau and P. Goullet, “Septicemia Caused by Propionibacterium granulosum in a Compromised Patient,” Journal of Clinical Microbiology, Vol. 25, No. 12, 1987, pp. 2405-2406.
[8] R. Chaudhry, B. Dhawan, A. Pandey, S. K. Choudhary and A. S. Kumar, “Propionibacterium granulosum: A Rare Cause of Endocarditis,” Journal of Infection, Vol. 41, No. 3, 2000, p. 284. doi:10.1053/jinf.2000.0728
[9] J. S. Brazier and V. Hall, “Propionibacterium propionicum and Infections of the Lacrimal Apparatus,” Clinical Infectious Diseases, Vol. 17, No. 5, 1993, pp. 892-893. doi:10.1093/clinids/17.5.892
[10] J. Downes and W. G. Wade, “Propionibacterium acidifaciens sp. nov., Isolated from the Human Mouth,” International Journal of Systematic and Evolutionary Microbiology, Vol. 59, No. 11, 2009, pp. 2778-2781. doi:10.1099/ijs.0.010470-0
[11] D. G. Pitcher and M. D. Collins, “Phylogenetic Analysis of Some LL-Diaminopimelic Acid-Containing Coryneform Bacteria from Human Skin: Description of Propionibacterium innocuum sp. nov,” FEMS Microbiology Letters, Vol. 68, No. 3, 1991, pp. 295-300.
[12] J. C. Torrey, “Bacteria Associated with Certain Types of Abnormal Lymph Glands,” The Journal of Medical Research, Vol. 34, 1916, pp. 65-80.
[13] E. Stackebrandt, P. Schumann, K. P. Schaal and N. Weiss, “Propionimicrobium gen. Nov. A New Genus to Accomodate Propionibacterium lymphophilum (Torrey 1916) Johnson and Cummins 1972, 1057AL as Propionimicrobium lymphophilum comb. nov.,” International Journal of Systematic and Evolutionary Microbiology, Vol. 52, 2002, pp. 1925-1927. doi:10.1099/ijs.0.02188-0
[14] A. Yokota, T. Tamura, M. Takeuchi, N. Weiss and E. Stackebrandt, “Transfer of Propionibacterium innocuum Pitcher and Collins 1991 to Propioniferax gen. nov. as Propioniferax innocua comb. nov,” International Journal of Systematic Bacteriology, Vol. 44, No. 3, 1994, pp. 579-582. doi:10.1099/00207713-44-3-579
[15] L. V. Holdeman, E. P. Cato and W. E. C. Moore, “Anaerobe Laboratory Manual,” Virginia Polytechnic Institute and State University, Blacksburg, 1977.
[16] M. Nakamura, I. Kametani, S. Higaki and T. Yamagishi, “Identification of Propionibacterium acnes by Polymerase Chain Reaction for Amplification of 16S Ribosomal RNA and Lipase Genes,” Anaerobe, Vol. 9, 2003, pp. 5-10. doi:10.1016/S1075-9964(03)00061-1
[17] P. Seng, M. Drancourt, F. Gouriet, B. La Scola, P. E. Fournier, J. M. Rolain and D. Raoult, “Ongoing Revolution in Bacteriology: Routine Identification of Bacteria by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry,” Clinical Infectious Diseases, Vol. 49, No. 4, 2009, pp. 543-551. doi:10.1086/600885
[18] S. B. La and D. Raoult, “Direct Identification of Bacteria in Positive Blood Culture Bottles by Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry,” Plos One, Vol. 4, No. 11, 2009, p. e8041. doi:10.1371/journal.pone.0008041
[19] M. Vargha, Z. Takats, A. Konopka and C. H. Nakatsu, “Optimization of MALDI-TOF MS for Strain Level Differentiation of Arthrobacter Isolates,” Journal of Microbiological Methods, Vol. 66, No. 3, 2006, pp. 399-409. doi:10.1016/j.mimet.2006.01.006
[20] E. Nagy, T. Maier, E. Urban, G. Terhes and M. Kostrzewa, “Species Identification of Clinical Isolates of Bacteroides by Matrix-Assisted Laser-Desorption/Ionization Time-of-Flight Mass Spectrometry,” Clinical Microbiology and Infection, Vol. 15, No. 8, 2009, pp. 796-802. doi:10.1111/j.1469-0691.2009.02788.x
[21] P. E. Fournier, C. Couderc, S. Buffet, C. Flaudrops and D. Raoult, “Rapid and Cost-Effective Identification of Bartonella Species Using Mass Spectrometry,” Journal of Medical Microbiology, Vol. 58, No. Pt 9, 2009, pp. 1154-1159. doi:10.1099/jmm.0.009647-0
[22] E. Vanlaere, K. Sergeant, P. Dawyndt, W. Kallow, M. Erhard, H. Sutton, D. Dare, B. Devreese, B. Samyn and P. Vandamme, “Matrix-Assisted Laser Desorption Ionisation-Time-of-Flight Mass Spectrometry of Intact Cells Allows Rapid Identification of Burkholderia cepacia Complex,” Journal of Medical Microbiology, Vol. 75, No. 2, 2008, pp. 279-286. doi:10.1016/j.mimet.2008.06.016
[23] R. Kolinska, M. Drevinek, V. Jakubu and H. Zemlickova, “Species Identification of Campylobacter jejuni ssp. jejuni and C. coli by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and PCR,” Folia Microbiologica, Vol. 53, No. 5, 2008, pp. 403-409. doi:10.1007/s12223-008-0061-7
[24] A. Grosse-Herrenthey, T. Maier, F. Gessler, R. Schaumann, H. Bohnel, M. Kostrzewa and M. Kruger, “Challenging the Problem of Clostridial Identification with Matrix-Assisted Laser Desorption and Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS),” Anaerobe, Vol. 14, No. 4, 2008, pp. 242-249. doi:10.1016/j.anaerobe.2008.06.002
[25] L. Skultety, L. Hernychova, E. Bereghazyova, K. Slaba and R. Toman, “Detection of Specific Spectral Markers of Coxiella burnetii Isolates by MALDI-TOF Mass Spectrometry,” Acta Virologica, Vol. 51, No. 1, 2007, pp. 55-58.
[26] S. Sauer, A. Freiwald, T. Maier, M. Kube, R. Reinhardt, M. Kostrzewa and K. Geider, “Classification and Identification of Bacteria by Mass Spectrometry and Computational Analysis,” Plos One, Vol. 3, No. 7, 2008, p. e2843. doi:10.1371/journal.pone.0002843
[27] D. Parisi, M. Magliulo, P. Nanni, M. Casale, M. Forina and A. Roda, “Analysis and Classification of Bacteria by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and a Chemometric Approach,” Analytical and Bioanalytical Chemistry, Vol. 391, No. 6, 2008, pp. 2127-2134. doi:10.1007/s00216-008-2161-2
[28] E. Seibold, T. Maier, M. Kostrzewa, E. Zeman and W. Splettstoesser, “Identification of Francisella tularensis by Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Fast, Reliable, Robust, and Cost-Effective Differentiation on Species and Subspecies Levels,” Journal of Clinical Microbiology, Vol. 48, No. 4, 2010, pp. 1061-1069. doi:10.1128/JCM.01953-09
[29] E. N. Ilina, A. D. Borovskaya, M. V. Serebryakova, V. V. Chelysheva, K. T. Momynaliev, T. Maier, M. Kostrzewa and V. M. Govorun, “Application of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Study of Helicobacter pylori,” Rapid Communications in Mass Spectrometry, Vol. 24, No. 3, 2010, pp. 328-334. doi:10.1002/rcm.4394
[30] C. Moliner, C. Ginevra, S. Jarraud, C. Flaudrops, M. Bedotto, C. Couderc, J. Etienne and P. E. Fournier, “Rapid Identification of Legionella Species by Mass Spectrometry,” Journal of Medical Microbiology, Vol. 59, No. Pt 3, 2010, pp. 273-284. doi:10.1099/jmm.0.014100-0
[31] S. B. Barbuddhe, T. Maier, G. Schwarz, M. Kostrzewa, H. Hof, E. Domann, T. Chakraborty and T. Hain, “Rapid Identification and Typing of Listeria Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry,” Applied and Environmental Microbiology, Vol. 74, No. 17, 2008, pp. 5402-5407. doi:10.1128/AEM.02689-07
[32] S. K. Gudlavalleti, A. K. Sundaram, J. Razumovski and V. Doroshenko, “Application of Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Rapid Identification of Neisseria Species,” Journal of Biomolecular Techniques, Vol. 19, No. 3, 2008, pp. 200-204.
[33] R. Dieckmann, R. Helmuth, M. Erhard and B. Malorny, “Rapid Classification and Identification of Salmonellae at the Species and Subspecies Levels by Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry,” Applied and Environmental Microbiology, Vol. 74, No. 24, 2008, pp. 7767-7778. doi:10.1128/AEM.01402-08
[34] E. Carbonnelle, J. L. Beretti, S. Cottyn, G. Quesne, P. Berche, X. Nassif and A. Ferroni, “Rapid Identification of Staphylococci Isolated in Clinical Microbiology Laboratories by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry,” Journal of Clinical Microbiology, Vol. 45, No. 7, 2007, pp. 2156-2161. doi:10.1128/JCM.02405-06
[35] R. Dieckmann, E. Strauch and T. Alter, “Rapid Identification and Characterization of Vibrio Species Using Whole-Cell MALDI-TOF Mass Spectrometry,” Journal of Applied Microbiology, Vol. 109, No. 1, 2010, pp. 199-211. doi:10.1111/j.1365-2672.2009.04647.x
[36] C. Friedrichs, A. C. Rodloff, G. S. Chhatwal, W. Schellenberger and K. Eschrich, “Rapid Identification of Viridans Streptococci by Mass Spectrometric Discrimination,” Journal of Clinical Microbiology, Vol. 45, No. 8, 2007, pp. 2392-2397. doi:10.1128/JCM.00556-07
[37] A. Mellmann, J. Cloud, T. Maier, U. Keckevoet, I. Ramminger, P. Iwen, J. Dunn, G. Hall, D. Wilson, P. Lasala, M. Kostrzewa and D. Harmsen, “Evaluation of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry in Comparison to 16S rRNA Gene Sequencing for Species Identification of Nonfermenting Bacteria,” Journal of Clinical Microbiology, Vol. 46, No. 6, 2008, pp. 1946-1954. doi:10.1128/JCM.00157-08
[38] N. Degand, E. Carbonnelle, B. Dauphin, J. L. Beretti, B. M. Le, I. Sermet-Gaudelus, C. Segonds, P. Berche, X. Nassif and A. Ferroni, “Identification of Non Fermenting Gram Negative Bacilli Isolated in Cystic Fibrosis by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry,” Journal of Clinical Microbiology, Vol. 46, 2008, pp. 3361-3367. doi:10.1128/JCM.00569-08
[39] C. S. Stingu, A. C. Rodloff, H. Jentsch, R. Schaumann and K. Eschrich, “Rapid Identification of Oral Anaerobic Bacteria Cultivated from Subgingival Biofilm by MALDI-TOF-MS,” Oral Microbiology and Immunology, Vol. 23, No. 5, 2008, pp. 372-376. doi:10.1111/j.1399-302X.2008.00438.x
[40] E. Carbonnelle, C. Mesquita, E. Bille, N. Day, B. Dauphin, J. L. Beretti, A. Ferroni, L. Gutmann and X. Nassif, “MALDI-TOF Mass Spectrometry Tools for Bacterial Identification in Clinical Microbiology Laboratory,” Clinical Biochemistry, Vol. 44, No. 1, 2010, pp. 104-109.
[41] A. Bizzini and G. Greub, “MALDI-TOF MS, a Revolution in Clinical Microbial Identification,” Clinical Microbiology and Infection, Vol. 16, No. 11, 2010, pp. 1614-1619. doi:10.1111/j.1469-0691.2010.03311.x
[42] E. Bessede, M. Angla-Gre, Y. Delagarde, H. S. Sep, A. Menard and F. Megraud, “Matrix-Assisted Laser-Desorption/Ionization Biotyper: Experience in the Routine of a University Hospital,” Clinical Microbiology and Infection, Vol. 17, 2010, pp. 533-538. doi:10.1111/j.1469-0691.2010.03274.x.
[43] M. Drancourt, C. Bollet, A. Carlioz, R. Martelin, J. P. Gayral and D. Raoult, “16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates,” Journal of Clinical Microbiology, Vol. 38, No. 10, 2000, pp. 3623-3630.
[44] N. Asseray, C. Papin, S. Touchais, P. Bemer, C. Lambert, D. Boutoille, B. Tequi, F. Gouin, F. Raffi, N. Passuti and G. Potel, “Improving Diagnostic Criteria for Propionibacterium acnes Osteomyelitis: A Retrospective Analysis,” Scandinavian Journal of Infectious Diseases, Vol. 42, No. 6-7, 2010, pp. 421-425. doi:10.3109/00365540903527330
[45] P. Seng, J. M. Rolain, P. E. Fournier, B. La Scola, M. Drancourt and D. Raoult, “MALDI-TOF-Mass Spectrometry Applications in Clinical Microbiology,” Future Microbiology, Vol. 5, 2010, pp. 1733-1754. doi:10.2217/fmb.10.127
[46] A. Croxatto, G. Prod’hom and G. Greub, “Applications of MALDI-TOF Mass Spectrometry in Clinical Diagnostic Microbiology,” FEMS Microbiology Reviews, Vol. 36, No. 2, 2011, pp. 380-407.

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