A Comparative Study of the Novel Ratio Difference Method versus Conventional Spectrophotometric Techniques for the Analysis of Binary Mixture with Overlapped Spectra


The smart novel ratio difference spectrophotometric method was developed and validated for the determination of a binary mixture of Sodium cromoglicate (SCG) and Fluorometholone (FLU) in presence of benzalkonium chloride without prior separation. The results were compared to that of the conventional methods (dual wavelength and first derivative of ratio spectra). The suggested methods were validated in compliance with the ICH guidelines and were successfully applied for determination of SCG and FLU in their laboratory prepared mixtures and commercial ophthalmic solution. The novel method showed significant advantages over the conventional methods regarding simplicity, minimal data manipulation and maximum reproducibility and robustness; which enabled the analysis of binary mixtures with overlapped spectra for routine quality control testing with quite satisfactory.

Share and Cite:

H. Lotfy, S. Saleh, N. Hassan and S. Elgizawy, "A Comparative Study of the Novel Ratio Difference Method versus Conventional Spectrophotometric Techniques for the Analysis of Binary Mixture with Overlapped Spectra," American Journal of Analytical Chemistry, Vol. 3 No. 11, 2012, pp. 761-769. doi: 10.4236/ajac.2012.311101.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] N. Y. Hassan, E. M. Abdel-Moety, N. A. Elragehy and M. R. Rezk, “Selective Determination of Ertapenem in the Presence of Its Degradation Product,” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 72, No. 5, 2009, pp. 915-921. doi:10.1016/j.saa.2008.12.025
[2] H. M. Lotfy, M. M. A. Abosen and M. G. El-Bardicy, “Stability-Indicating Methods for the Determination of Famciclovir in the Presence of Its Alkaline-Induced Degradation Product,” Drug Testing and Analysis, Vol. 2, No. 4, 2010, pp. 188-199. doi:10.1002/dta.119
[3] N. Aguerssif, M. Benamor, M. Kachbi and M. T. Draa, “Simultaneous Determination of Fe(III) and Al(III) by First-Derivative Spectrophotometry and Partial Least-Squares (PLS-2) Method—Application to Post-Haemodialysis Fluids,” Journal of Trace Elements in Medicine and Biology, Vol. 22, No. 3, 2008, pp. 175-182. doi:10.1016/j.jtemb.2007.12.004
[4] N. E. Wagieh, M. A. Hegazy, M. Abdelkawy and E. A. Abdelaleem, “Quantitative Determination of Oxybutynin Hydrochloride by Spectropho-tometry, Chemometry and HPTLC in Presence of Its Degradation Product and Additives in Different Pharmaceutical Dosage Forms,” Talanta, Vol. 80, No. 5, 2010, pp. 2007-2015. doi:10.1016/j.talanta.2009.11.002
[5] H. W. Darwish, S. A. Hassan, M. Y. Salem and B. A. El-Zeiny, “Three Different Spectrophotometric Methods Manipulating Ratio Spectra for Determination of Binary Mixture of Amlodipine and Atorvastatin,” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 83, No. 1, 2011, pp. 140-148.
[6] M. G. El-Bardicy, H. M. Lotfy, M. A. El-Sayed and M. F. El-Tarras, “Smart Stability-Indicating Spectrophotometric Methods for Determination of Binary Mixtures without Prior Separation,” Journal of AOAC International, Vol. 91, No. 2, 2008, pp. 299-310.
[7] N. S. Abdelwahab, “Spectrophotometric Methods for Simultaneous Determination of Carvedilol and Hydro- chlorothiazide in Combined Dosage Form,” Arabian Journal of Chemistry, 2011.
[8] N. K. Ramadan, H. M. Mohamed and A. A. Moustafa, “Simultaneous Determination of Rabeprazole Sodium and Domperidone,” Journal of Applied Pharmaceutical Science, Vol. 1, No. 9, 2011, pp. 73-80.
[9] S. C. Sweetman, “Martindale: The Complete Drug Reference,” Pharmaceutical Press, London, 2005.
[10] “British Pharmacopoeia,” The Stationery Office on Behalf of the Medicines and Healthcare Products Regulatory Agency (MHRA)-? Crown Copyright, 2009.
[11] H. M. Lotfy and M. A. Hagazy, “Comparative Study of Novel Spectrophotometric Methods Manipulating Ratio Spectra: An Application on Pharmaceutical Ternary Mixture of Omeprazole, Tinidazole and Clarithromycin,” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 96, 2012, pp. 259-270. doi:10.1016/j.saa.2012.04.095
[12] D. Harvey, “Modern Analytical Chemistry,” McGraw-Hill, New York, 2000.
[13] H. M. Lotfy, “Simultaneous Determnination of Omeprazole, Tinidazole and Clarithromycin in Combination,” Bulletin of Faculty of Pharmacy, Cairo University, Vol. 44, No. 2, 2006. pp. 27-38.
[14] F. Salinas, J. J. Berzas-Nevado and A. E. Mansilla, “A New Spectrophotometric Method for Quantitative Multicomponent Analysis Resolution of Mixtures of Salicylic and Salicyluric Acids,” Talanta, Vol. 37, Suppl. 3, 1990, pp. 347-351. doi:10.1016/0039-9140(90)80065-N
[15] Quality Control Department, “Test Method for Determination of Fluca Ear Drops,” National Organization for Drug Control and Research, Egypt.
[16] International Conference on Harmonization (ICH) Q2B, “Validation of Analytical Procedures: Methodology,” Federal Register, Vol. 62, No. 96, 1997, pp.27463-27467.

Copyright © 2021 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.