Effect of Pleurotus ostreatus on hyperglycemia, DNA damage and chromosomes aberrations


One of the main health problems with high and markedly increased complications is diabetes. Despite several projects with preventative strategies and armories of medication, the arrangement of diabetes remains grossly unsatisfactory. Thus, it is vital to identify unfamiliar drugs or novel nutraceuticals for treating and preventing diabetes without side effects. The present study deals with scientific information on mushrooms with regards to its potential use as anti-diabetic active food. In addition to the anti-hyperglycemic action of mushrooms, the present study presents its effect on DNA damage, chromosome aberrations and sperm alternations in streptozotocin-induced diabetic rats. These animals have been treated, for 30 days, with amaryl (as control treatment) (0.03 mg/kg·b·wt/dl), low-dose mushroom (100 mg/kg·b·wt/dl) and high-dose mushroom (200 mg/ kg·b·wt/dl). The glucose level GL of streptozotocin-induced diabetic animals has been markedly improved by mushroom treatment; for example GL has decreases from 167.6 mg/dl down to 116.0 mg/dl for treatment with high-dose mushroom and 128.9 mg/dl for treatment with low-dose mushroom, comparing with amaryl treatment that decreases GL down to 92.6 mg/dl. But, the experimental results show that treatment with mushroom is better than treatment with amaryl in case of genetic changes (DNA fragmentation, disappear of some base pairs and chromosome aberrations. So, it is proposed that more close scientific attention be paid to precede more research of functional mushrooms for preventive and curative treatments for diabetes.

Share and Cite:

Al-Malki, A. (2012) Effect of Pleurotus ostreatus on hyperglycemia, DNA damage and chromosomes aberrations. Journal of Biomedical Science and Engineering, 5, 657-663. doi: 10.4236/jbise.2012.511082.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Wild, S., Roglic, G., Green, A., Sicree, R. and King, H. (2004) Global prevalence of diabetes: Estimate for 2000 and projection of 2030. Diabetes Care, 27, 1047-1053.
[2] Li, W.L., Zheng, H.C., Bukuru, J. and De Kimpe, N. (2004) Natural medicines used in traditional Chinese medical system for therapy of diabetic mellitus. Journal of Ethnopharmacology, 92, 1-21.
[3] Chang S.T. and Miles P.G. (1992) Mushrooms biology a new discipline. Mycologist, 6, 64-65. doi:10.1016/S0269-915X(09)80449-7
[4] Hawksworth D.L. (2001) Mushrooms: The extent of unexplored potential. International Journal of Medical Mushrooms, 3, 333-337.
[5] Shu-Ting, C. and Philip, G.M. (2004) Tremella—Increased production by a mixed culture technique. In: Mushrooms: Cultivation, Nutritional Value Medical Effect and Environmental Impact, 2nd Edition, CRC Press, Boca Raton.
[6] Kiho, T., Tsujimura, Y., Sakushima, M., Usui, S. and Ukai, S. (1994) Polysaccharides in fungi. XXXIII. Hypoglycemic activity of an acidic polysaccharide (AC) Tremella fuciformis. Yakugaku zasshi, 114, 308-315.
[7] Cho, E.J., Hwang, H.J., Kim, S.W., Oh, J.Y., Baek. Y.M., Choi, J.W., et al. (2007) Hypoglycemic effects of exopolysaccharides produced by mycelia cultures of two different mushrooms Tremella fuciformis and Phellinus baumii in ob/ob mice. Applied Microbiology and Biotechnology, 75, 1257-1265.
[8] Nieszner, E., Posa, I., Kocsis, E., Pogatsa, G., Preda, I. and Koltai M.Z. (2002) Influence of different sulfonylureas on the size of myocardial infarction with and without ischemic preconditioning in rabbits. Experimental Clinical Endocrinology Diabetes, 110, 2002, 212-218.
[9] Gray, A.M. and Flatt, P.R. (1998) Insulin-releasing and insulin-like activity of Agaricus campestris (mushroom). Journal of Endocrinology, 157, 259-266.
[10] Hokama, Y. and Hokama, J.L. (1981) In vitro ihibitation of platelet aggregation with low Dalton compounds from aqueous dialysates of edible fungi. Research Communications in Chemical Pathology and Pharmacology, 31, 177-180.
[11] Jose, N. and Janardhanan, K.K. (2000) Antioxidant and antitumor activity of Pleurotus florida. Current Science, 79, 941-943.
[12] Szkudelski, T. (2001) The mechanism of alloxan and streptozotocin action in Beta cells of the rat pancreas. Physiological Research, 50, 537-546.
[13] Pihlanto, A. (2006) Anti-oxidative peptides derived from milk proteins. International Dairy Journal, 16, 2006, 1306-1314.
[14] Rabbani, S.I., Devi, K. and Khanam, S. (2009) Inhibitory effect of glimepiride on nicotinamide-streptozotocin induced nuclear damage and sperm abnormality in diabetic Wister rats. Indian Journal Experimental Biology, 47, 804-810.
[15] Valko, M., Leibfritz, D., Moncol, J., Croni, M.T., Mazur M. and Tesler, J. (2007) Free radicals and antioxidant in normal physiological function and human diseases. International Journal of Biochemistry and Cell Biology, 39, 2007, 44-84.
[16] Otton, R., Soriano, F.G., Verlengia, R. and Curi, R. (2004) Diabetes induces apoptosis in lymphocytes. Journal of Endocrinology, 182, 145-156. doi:10.1677/joe.0.1820145
[17] Abd El-Rahim, A.H., Radwan, H.A., Abd El-Moneim, O.M., Farag, I.M. and Nada, S.A. (2010) The influence of amaryl on genetic alterations and sperm abnormalities of rats with alloxan-induced hyperglycemia. Journal American Science, 6, 1739-1748.
[18] Tepe, G., Dietrich, T., Grafen, F., Brehme, U., Muschick, P. and Dinkelborg, L.M. (2005) Reduction of intimal hyperplasia with Re-188-labeled stents in a rabbit model at 7 and 26 weeks: An experimental study. Cardiovascular and Interventional Radiology, 28, 623-627.
[19] Wasser, S.P. and Weiss, A.L. (1999) Therapeutic effects of substances occurring in higher Basidiomycetes mush-rooms: A modern perspective. Critical Reviews in Immunology, 19, 65-96.
[20] Ajith, T., Janardhanan, K.K., Antioxidant and anti-inflammatory activities of methanol extract of Phellinus rimosus (Berk) Pilat. Indian Journal of Experimental Biology, 39, 1166-1169.
[21] Aletor, V.A. (1995) Compositional studies on edible tropical species of mushrooms. Food Chemistry, 54, 265- 268.
[22] Lin, S., Zhu, P. and Liao, H. (1998) Blocking effect of edible mushroom beverages on the carbon tetrachloride induced rat liver lipid per-oxidation, Zhongguo Gonggong Weisheng Xuebao, 17, 15.
[23] Jayakumar, T., Ramesh, E. and Geraldine, P. (2006) Anti-oxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCl(4)-induced liver injury in rats. Food and Chemical Toxicology, 44, 1989-1996.
[24] Tuzcu, M. and Baydas, G. (2006) Effect of melatonin and vitamin E on diabetes-induced learning and memory impairment in rats. European Journal of Pharmacology, 537, 106-110.
[25] Kuhad, A., Sethi, R. and Chopra, K. (2008) Lycopene attenuates diabetes-associated cognitive decline in rats. Life Sciences, 83, 128-134.
[26] Gibb, A., Taylar, D.D., Wan, T., Oconnor, D.M., Doering, D.L. and Gercel-Taylor, C. (1997) Apoptosis as a measure of chemosensitivity to cisplatin and taxol therapy in ovarian cancer cell lines. Gynecologic Oncology, 65, 13- 22
[27] Sharma, K.K., Lavanya, M. and Anjaiah, V. (2000) A method for isolation and purification of peanut genomic DNA suitable for analytical applications. Plant Molecular Biology Reporter, 18, 393-398.
[28] Williams, D.L., Harris, A., Williams, K.J., Brosius, M.J. and Lemods W. (1984) A direct bone marrow chromosome technique for acute lymphoblastic ISSR markers. International Journal of Agriculture and Biological Leukemia, Cancer Genetics Cytogenetics, 13, 239-257.
[29] Zietkiewicz, E., Rafalski, A. and Labuda, D. (1994) Genome finger-printing by simple repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20, 176-183.
[30] Preston, R.J., Dean, B.D., Galloway, S., Holden, H., McFee, A.F. and Shelly, M. (1987) Mammalian in vivo cytogenetic assays: Analysis of chromosome aberrations in bone marrow cells. Mutation Research, 189, 157-165.
[31] Wyrobek, A.J. and Bruce, W.R. (1978) The induction of sperm shape-abnormalities in mice and humans. Chemical Mutagens, 5, 257-285.
[32] Narayana, K. (2008) An amino-glycoside antibiotic gentamycin induced oxidative stress, reduces antioxidant reserve and impairs spermatogenesis in rats. Journal of Toxicological Sciences, 33, 85-96.
[33] Endo, T.R. (1988) Induction of chromosomal structural changes by a chromosome of Aegilops cylindrical L in common wheat. Journal of Heredity, 79, 366-370.
[34] Mattila, P., Salo-V??n?nen, P., K?nk?, K., Aro, H. and Jalava, T. (2002) Basic composition and amino acid contents of mushrooms cultivated in Finland. Journal of Agricultural and Food Chemistry, 50, 6419-6422. doi:10.1021/jf020608m
[35] Arulmozhi, D.K., Veeranjaneyulu, A. and Bodhankar, S.L. (2004) Neonatal streptozotocin-induced rat model of type 2 diabetes mellitus: A glance. Indian Journal of Pharmacology, 36, 217-221.
[36] Portha, B., Picolon, L. and Rosselin, G. (1979) A Chemical diabetes in the adult rats as the spontaneous evolution of neonatal diabetes. Diabetologia, 17, 371-377. doi:10.1007/BF01236272
[37] Weir, C.G., Clore, E.E., Zma-Chinsky, C.J. and Bonnier-Wier, S. (1981) Islet secretion in a new experimental model for non-insulin-dependent diabetes. Diabetes Metabolism Reviews, 30, 590-595.
[38] Block, K.O., Zemel, R., Bloch, O.V., Grief, H. and Vardi, P. (2000) Streptozotocin and alloxan-based selection improve toxin resistance of insulin producing RINm Cells. International Journal of Experimental Diabetes Research, 1, 211-219. doi:10.1155/EDR.2000.211
[39] Rehman, A., Nourooz-Zadeh, J., Moller, W., Tritschler, H., Pereira, P. and Halliwell, B. (1999) Increased oxidative damage to all DNA bases in patients with type II diabetic mellitus. FEBS Letters, 448, 120-122. doi:10.1016/S0014-5793(99)00339-7
[40] Aydin, S., Aytac, E., Uzun, H., Altug, T., Mansur, B., Saygili, S., Buyukpinarbasili, N. and Sariyar, M. (2010) Effects of ganderma lucidum on obstructive jaundice-induced oxidative stress. Asian Journal of Surgery, 33, 173-180.
[41] Piconi, L., Quagliaro, L. and Ceriello, A. (2003) Oxidative stress in diabetes. Clinical Chemistry and Laboratory Medicine, 41, 1144-1149.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.