Development of H5 subtype-specific monoclonal antibodies (MAb) and MAb-based assays for rapid detection of H5 avian influenza

Huaguang Lu, Lin Lin, Ronghui Wang, Yanbin Li, Yanbin Li, Bill Scheuchenzuber, Jiabo Liu, Zhiqin Xie, Joseph A. Rosebrock

Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, USA.
Guangxi Veterinary Research Institute, Nanning, China.
ImmTech, Incorporated, 206 High St., New Windsor, USA.
Wiley Lab/Avian Virology, Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, USA.

DOI: 10.4236/health.2012.430141                

Abstract

Avian influenza (AI) virology surveillance is the most important method to monitor AI virus (AIV) in poultry so as to effectively prevent and control AI outbreaks. Monoclonal antibodies (MAb)-based assays are highly sensitive and specific for AIV detection, and much practical and economic for test-in-field or onsite. Many such assays have been developed and are still in developing since the H5N1 highly pathogenic AI (HPAI) outbreaks occurred in South East Asia in 2003. A MAb-based dot-enzyme-linked immunosorbent assay (ELISA) has been developed in our lab during late 1990s and early 2000s. Meanwhile, AIV H7 and H5 subtype specific-MAbs have been successfully developed in our laboratory to enhance the Dot-ELISA and other MAb-based assays for AIV detection. Production and purification of the H7 and H5 MAbs were made to provide essential reagents for Dot-ELISA and other immunoassays, and the current development of a novel Biosensor technique for rapid detection of AIV from clinical and field specimens.

Keywords

Avian Influenza Virus; Hemagglutinin; Hybridoma Cell Line, Monoclonal Antibodies; Dot-ELISA; Biosensor

Conflicts of Interest

The authors declare no conflicts of interest.

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