Separation of Amino Acids Based on Thin-Layer Chromatography by a Novel Quinazoline Based Anti-Microbial Agent

Supriti Sen, Sandipan Sarkar, Pijush Kundu, Subrata Laskar

Department of Chemistry, The University of Burdwan, Golapbag, India.

DOI: 10.4236/ajac.2012.39088                

Abstract

A newly designed quinazoline based compound, 6-pyridin-2-yl-5,6-dihydro-benzo[4,5]imidazo[1,2-c] quinazoline (PDBIQ) has shown the ability for the easy detection of nineteen amino acids on thin-layer chromatography plates as a spray reagent. This new reagent enabled to produce various distinguishable colors with amino acids with different R_F values. The detection limits and the binding ability of PDBIQ with amino acids have been calculated. PDBIQ is also able to detect aminoacids from hydrolised seed protein. The title compound also exhibited profound inhibitory action against some gm (+ve) and gm (-ve) bacterial organisms. This paper deals with synthesis, spectroscopic application and biological evaluation of the organic moity.

Keywords

Thin-Layer Chromatography; Amino Acid; Ninhydrin; Binding Constant; Antimicrobial Property

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	S. Laskar and S. Lahiri, “An Account on Color Reagents for Detection of Amino Acids and Interaction of Metal Ions with Some Non-Conventional Seed Proteins,” In: G. Brahmachari, Ed., Natural Products: Chemistry, Biochemistry and Pharmacology, Narosa Publishing House Pvt. Ltd., New Delhi, 2009, pp. 676-702.
[2]	V. Alagarsamy, V. R. Solomon, M. Murugan, R. Sankaranarayanan, R. Periyasamy and R. Deepa, “Synthesis of 3-(2-Pyridyl)-2-substituted-quinazolin-4(3H)-ones as New Analgesic and Anti-Inflammatory Agents,” Biomedicine & Pharmacotherapy, Vol. 62, No. 7, 2008, pp. 454-461. HUdoi:10.1016/j.biopha.2006.10.003U
[3]	R. Rohini, K. Shanker, P. M. Reddy, A. Hu and V. Ravinder, “Anti Microbial Study of Newly Synthesized 6- Substituted Indolo[1,2-c]Quinazolines,” European Journal of Medicinal Chemistry, Vol. 41, No. 28, 2010, pp. 1200-1205. HUdoi:10.1002/chin.201028163U
[4]	G. M. Higa and J. Abraham, “Lapatinib in the Treatment of Breast Cancer,” Expert Review of Anticancer Therapy, Vol. 7, No. 9, 2007, pp. 1183-1192. HUdoi:10.1586/14737140.7.9.1183U
[5]	H. R. Mahler and E. H. Cordes, “Basic Biological Chemistry,” Harper and Row, New York, 1968.
[6]	C. Haworth and J. G. Heathcote, “The Direct Determination of Amino Acids on Thin-Layer Chromatograms by Densitometry,” Biochemistry Journal, Vol. 114, No. 3, 1969, pp. 667-668. HUdoi:10.1016/S0021-9673(00)99169-6U
[7]	K. Lorentz and B. Flatter, “Staining of Amino Acids with Benzoquinone in Paper Chromatography,” Analalytical Biochemistry, Vol. 38, No. 2, 1970, pp. 557-559. HUdoi:10.1007/s007260170027U
[8]	B. Basak and S. Laskar, “Spray Reagents for the Detection of Amino-Acids on Thin-Layer Plates,” Talanta, Vol. 37, No. 11, 1990, pp. 1105-1106. HUdoi:10.1016/0039-9140(90)80163AU
[9]	S. Laskar, U. Bhattacharya and B. Basak, “Modified Ninhydrin Spray Reagent for the Identification of Amino Acids on Thin-Layer Chromatography Plates,” Analyst Vol. 116, No. 6, 1991, pp. 1105-1106. HUdoi:10.1039/an9911600625U
[10]	T. D. Samanta and S. Laskar, “New Reagent for Detection of Amino Acids on TLC Plates,” Journal of Planar Chromatography, Vol. 19, No. 109, 2006, pp. 252-254. HUdoi:10.1556/JPC.19.2006.3.17U
[11]	A. M. Pyle, J. P. Rehmann, R. Meshoyrer, C. V. Kumar, N. J. Turro and J. K. Barton, “Mixed-Ligand Complexes of Ruthenium(II): Factors Governing Binding to DNA,” Journal of American Chemical Socity, Vol. 111, No. 8, 1989, pp. 3051-3058. HUdoi:10.1021/ja00190a046U
[12]	S. Bhattacharya, S. Bhattacharya (Banerjee), K. Ghosh, S. Basu, M. Banerjee, “Study of Electron Donor-Acceptor Complex Formation of o-Chloranil With a Series of Phosphine Oxides and Trin-Butyl Phosphate by the Absorption Spectrometric Metric method,” Journal of Solution Chemistry, Vol. 35, No. 4, 2006, pp. 519-539. HUdoi:10.1007/s10953-005-9013U
[13]	R. Cruickshank, J. P. Duguid, B. P. Marmion and R. H. A. Swain, “Medical Microbiology,” 12th Edition, Edward Arnold Publishers, Vol. II, 1975.