Antibacterial activity of Ampicillin trihydrate formulated in Aluminium-Magnesium Silicate, against Salmonella gallinarum

Maduike C. O. Ezeibe, George N. Anosa, Okechi K. Okorie, Nnenna P. Elendu-Eleke, Obianuju N. Okoroafor, Augustine A. Ngene, Ogechukwu N. Chikelu

Department of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria.

DOI: 10.4236/health.2012.49106                

Abstract

To test if stabilizing Ampicillin trihydrate (AT) with Aluminium-Magnesium Silicate (AMS) can enhance its antibacterial activities, different concentrations of AT solution and of a formulation of AT in the AMS, were made and used for sensitivity test on Salmonella gallinarum cultures. Also, S. gallinarum-infected chicks were treated with; 10 mg/Kg (AT), 10 mg/Kg (AT in AMS), 7.5 mg/Kg (AT), 7.5 mg/Kg (AT in AMS). Mean diameter of inhibition zone, 28.39 ± 2.07 mm produced by AT did not vary significantly (P > 0.05) from 26.36 ± 2.05 mm produced by AT in AMS. However, mean Salmonella gallinarum culture forming units per ml of bile, 17.6 ± 11 × 10⁵of the untreated chicks and 3.4 ± 0.81 × 10⁵(80.58% reduction), 2.4 ± 0.67 × 10⁵(85.70% reduction), 5.4 ± 1.93 × 10⁵(69.20% reduction ) and 0.38 ± 0.13 × 10⁵(97.80% reduction ) of the respective treated groups, showed AMS significantly (P < 0.01) improved AT’s ability to clear S. gallinarum infection, in vivo.

Keywords

Antibiotic Resistance; Synthetic Aluminium-Magnesium Silicate; Stabilization; Salmonella gallinarum

Conflicts of Interest

The authors declare no conflicts of interest.

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